Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model

Collagen-induced arthritis in DBA/1 mice is a model of rheumatoid arthritis with marked synovitis and erosions. The disease can be adoptively transferred to SCID mice with arthritogenic splenocytes from DBA/1 mice injected with bovine collagen type II. However, infection of arthritogenic splenocytes...

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Autores principales:	Ledda, María Fernanda, Podhajcer, Osvaldo Luis
Publicado:	1997
Materias:	Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09697128_v4_n6_p553_Chernajovsky http://hdl.handle.net/20.500.12110/paper_09697128_v4_n6_p553_Chernajovsky
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09697128_v4_n6_p553_Chernajovsky
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spelling	paper:paper_09697128_v4_n6_p553_Chernajovsky2023-06-08T15:59:04Z Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model Ledda, María Fernanda Podhajcer, Osvaldo Luis Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus Collagen-induced arthritis in DBA/1 mice is a model of rheumatoid arthritis with marked synovitis and erosions. The disease can be adoptively transferred to SCID mice with arthritogenic splenocytes from DBA/1 mice injected with bovine collagen type II. However, infection of arthritogenic splenocytes with a retrovirus expressing TGF β1 inhibits development of arthritis in SCID mice. When DBA/1 mice, at onset of arthritis have additional arthritgenic splenocytes transferred, exacerbation occurs, reflected in a rapid increase in the number of arthritic joints, increased paw swelling and higher levels of anti-collagen antibody. By infecting arthritogenic splenocytes ex vivo with TGF β1 retrovirus, this exacerbation was inhibited. TGF β1 was effective in lowering inflammation of joints with already established arthritis and inhibiting the spreading of the disease to other joints. Transient reduction on anti-collagen antibody levels could also be obtained using purified T cells infected woth TGF β1 retrovirus. In addition, expression of TGF β1 in lymphocytes reduced the levels of gelatinase (MMP2) activity in inflamed joints. Fil:Ledda, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Podhajcer, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09697128_v4_n6_p553_Chernajovsky http://hdl.handle.net/20.500.12110/paper_09697128_v4_n6_p553_Chernajovsky
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus
spellingShingle	Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus Ledda, María Fernanda Podhajcer, Osvaldo Luis Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model
topic_facet	Cytokine receptors Cytokines Rheumatoid arthritis T lymphocytes TGF β1 retrovirus collagen collagen antibody gelatinase transforming growth factor beta1 animal cell animal experiment animal model animal tissue antiinflammatory activity article enzyme activity gene transfer lymphoid cell male mouse nonhuman paw edema priority journal protein expression Retrovirus rheumatoid arthritis spleen cell synovitis T lymphocyte Animalia Bovinae unidentified retrovirus
description	Collagen-induced arthritis in DBA/1 mice is a model of rheumatoid arthritis with marked synovitis and erosions. The disease can be adoptively transferred to SCID mice with arthritogenic splenocytes from DBA/1 mice injected with bovine collagen type II. However, infection of arthritogenic splenocytes with a retrovirus expressing TGF β1 inhibits development of arthritis in SCID mice. When DBA/1 mice, at onset of arthritis have additional arthritgenic splenocytes transferred, exacerbation occurs, reflected in a rapid increase in the number of arthritic joints, increased paw swelling and higher levels of anti-collagen antibody. By infecting arthritogenic splenocytes ex vivo with TGF β1 retrovirus, this exacerbation was inhibited. TGF β1 was effective in lowering inflammation of joints with already established arthritis and inhibiting the spreading of the disease to other joints. Transient reduction on anti-collagen antibody levels could also be obtained using purified T cells infected woth TGF β1 retrovirus. In addition, expression of TGF β1 in lymphocytes reduced the levels of gelatinase (MMP2) activity in inflamed joints.
author	Ledda, María Fernanda Podhajcer, Osvaldo Luis
author_facet	Ledda, María Fernanda Podhajcer, Osvaldo Luis
author_sort	Ledda, María Fernanda
title	Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model
title_short	Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model
title_full	Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model
title_fullStr	Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model
title_full_unstemmed	Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model
title_sort	pathogenic lymphoid cells engineered to express tgf β1 ameliorate disease in a collagen-induced arthritic model
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09697128_v4_n6_p553_Chernajovsky http://hdl.handle.net/20.500.12110/paper_09697128_v4_n6_p553_Chernajovsky
work_keys_str_mv	AT leddamariafernanda pathogeniclymphoidcellsengineeredtoexpresstgfb1amelioratediseaseinacollageninducedarthriticmodel AT podhajcerosvaldoluis pathogeniclymphoidcellsengineeredtoexpresstgfb1amelioratediseaseinacollageninducedarthriticmodel
_version_	1768541663158861824

Pathogenic lymphoid cells engineered to express TGF β1 ameliorate disease in a collagen-induced arthritic model

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