Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes
The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l−1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that...
| Autores principales: | , , , |
|---|---|
| Publicado: |
2017
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v119_n_p605_Ferreira http://hdl.handle.net/20.500.12110/paper_09648305_v119_n_p605_Ferreira |
| Aporte de: |
| id |
paper:paper_09648305_v119_n_p605_Ferreira |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_09648305_v119_n_p605_Ferreira2023-06-08T15:58:34Z Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes Casabuono, Adriana Cristina Couto, Alicia Susana Ramirez, Silvana Andrea María Vullo, Diana Lía Bacterial lipopolysaccharide (LPS) Biotreatment Cadmium retention Extracellular polymeric substances (EPS) Pseudomonas veronii 2E Bacteria Binding sites Chromatography Glucose Industrial water treatment Ligands Oxalic acid Polymers Polysaccharides Titration Voltammetry Anodic stripping voltammetry Biotreatments Extracellular polymeric substances High-performance anion-exchange chromatography Industrial wastewater treatment Lipopolysaccharides Pseudomonas veronii Pulsed amperometric detection Wastewater treatment bacterium bioactivity cadmium chemical analysis concentration (composition) exopolymer hydrolysis ligand oxalic acid polysaccharide purification waste treatment wastewater treatment Bacteria (microorganisms) Pseudomonas veronii The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l−1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that EPS consisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, Proteinase K and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides (LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronic acid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of the secreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fraction contained the highest ligand concentration (279 ± 10 μmoles Cd(II) binding sites g LPS−1). This information is a valuable tool for the application of EPS in industrial wastewater treatment. © 2016 Elsevier Ltd Fil:Casabuono, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramirez, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vullo, D.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v119_n_p605_Ferreira http://hdl.handle.net/20.500.12110/paper_09648305_v119_n_p605_Ferreira |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacterial lipopolysaccharide (LPS) Biotreatment Cadmium retention Extracellular polymeric substances (EPS) Pseudomonas veronii 2E Bacteria Binding sites Chromatography Glucose Industrial water treatment Ligands Oxalic acid Polymers Polysaccharides Titration Voltammetry Anodic stripping voltammetry Biotreatments Extracellular polymeric substances High-performance anion-exchange chromatography Industrial wastewater treatment Lipopolysaccharides Pseudomonas veronii Pulsed amperometric detection Wastewater treatment bacterium bioactivity cadmium chemical analysis concentration (composition) exopolymer hydrolysis ligand oxalic acid polysaccharide purification waste treatment wastewater treatment Bacteria (microorganisms) Pseudomonas veronii |
| spellingShingle |
Bacterial lipopolysaccharide (LPS) Biotreatment Cadmium retention Extracellular polymeric substances (EPS) Pseudomonas veronii 2E Bacteria Binding sites Chromatography Glucose Industrial water treatment Ligands Oxalic acid Polymers Polysaccharides Titration Voltammetry Anodic stripping voltammetry Biotreatments Extracellular polymeric substances High-performance anion-exchange chromatography Industrial wastewater treatment Lipopolysaccharides Pseudomonas veronii Pulsed amperometric detection Wastewater treatment bacterium bioactivity cadmium chemical analysis concentration (composition) exopolymer hydrolysis ligand oxalic acid polysaccharide purification waste treatment wastewater treatment Bacteria (microorganisms) Pseudomonas veronii Casabuono, Adriana Cristina Couto, Alicia Susana Ramirez, Silvana Andrea María Vullo, Diana Lía Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
| topic_facet |
Bacterial lipopolysaccharide (LPS) Biotreatment Cadmium retention Extracellular polymeric substances (EPS) Pseudomonas veronii 2E Bacteria Binding sites Chromatography Glucose Industrial water treatment Ligands Oxalic acid Polymers Polysaccharides Titration Voltammetry Anodic stripping voltammetry Biotreatments Extracellular polymeric substances High-performance anion-exchange chromatography Industrial wastewater treatment Lipopolysaccharides Pseudomonas veronii Pulsed amperometric detection Wastewater treatment bacterium bioactivity cadmium chemical analysis concentration (composition) exopolymer hydrolysis ligand oxalic acid polysaccharide purification waste treatment wastewater treatment Bacteria (microorganisms) Pseudomonas veronii |
| description |
The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances (EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in medium M9-14 g l−1 glycerol, 25 °C. Spectrophotometric and potentiometric titration studies showed that EPS consisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, Proteinase K and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides (LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronic acid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of the secreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fraction contained the highest ligand concentration (279 ± 10 μmoles Cd(II) binding sites g LPS−1). This information is a valuable tool for the application of EPS in industrial wastewater treatment. © 2016 Elsevier Ltd |
| author |
Casabuono, Adriana Cristina Couto, Alicia Susana Ramirez, Silvana Andrea María Vullo, Diana Lía |
| author_facet |
Casabuono, Adriana Cristina Couto, Alicia Susana Ramirez, Silvana Andrea María Vullo, Diana Lía |
| author_sort |
Casabuono, Adriana Cristina |
| title |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
| title_short |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
| title_full |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
| title_fullStr |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
| title_full_unstemmed |
Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes |
| title_sort |
chemical characterization of pseudomonas veronii 2e soluble exopolymer as cd(ii) ligand for the biotreatment of electroplating wastes |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v119_n_p605_Ferreira http://hdl.handle.net/20.500.12110/paper_09648305_v119_n_p605_Ferreira |
| work_keys_str_mv |
AT casabuonoadrianacristina chemicalcharacterizationofpseudomonasveronii2esolubleexopolymerascdiiligandforthebiotreatmentofelectroplatingwastes AT coutoaliciasusana chemicalcharacterizationofpseudomonasveronii2esolubleexopolymerascdiiligandforthebiotreatmentofelectroplatingwastes AT ramirezsilvanaandreamaria chemicalcharacterizationofpseudomonasveronii2esolubleexopolymerascdiiligandforthebiotreatmentofelectroplatingwastes AT vullodianalia chemicalcharacterizationofpseudomonasveronii2esolubleexopolymerascdiiligandforthebiotreatmentofelectroplatingwastes |
| _version_ |
1768544049198792704 |