Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
Optimization of laccase production in white-rot fungi has been extensively studied. Metallic and aromatic compounds have been found to enhance enzyme production, but the development of bioremediation as an application field of this enzyme requires clean technologies. In this work, co-cultivation of...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v104_n_p238_Kuhar http://hdl.handle.net/20.500.12110/paper_09648305_v104_n_p238_Kuhar |
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paper:paper_09648305_v104_n_p238_Kuhar2023-06-08T15:58:33Z Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation Castiglia, Valeria Carolina Levin, Laura Noemi Co-cultivation Laccase Malachite green White-rot fungi Bioremediation Biotechnology Carbonate minerals Cellulose Detoxification Fermentation Fungi Lignin Wood Cellulose and hemicellulose Co-cultivation Laccases Large-scale applications Malachite green Solid-state fermentation Trametes versicolor White rot fungi Enzymes bioremediation color enzyme activity fermentation fungal disease fungus malachite monoculture optimization Fermentation Laccase Production Fungi Ganoderma lucidum Trametes versicolor Optimization of laccase production in white-rot fungi has been extensively studied. Metallic and aromatic compounds have been found to enhance enzyme production, but the development of bioremediation as an application field of this enzyme requires clean technologies. In this work, co-cultivation of Ganoderma lucidum and Trametes versicolor was performed, showing remarkable enhancement of laccase activity. Dual cultures were assayed for malachite green degradation (MG) in solid state fermentation (SSF) using a sawdust-based medium. The time for achieving complete decolorization of MG in co-cultivation was markedly shorter than that observed in monocultures. Dual-species treatment did not differ in wood dry weight loss and lignin, cellulose and hemicellulose degradation, compared to monocultures; but the selectivity index (lignin loss/cellulose loss) of dual cultures was markedly higher than those attained by monocultures. Moreover, a modified isoenzymatic laccase pattern was observed, showing one isoenzyme that was absent in monocultures. Dual cultures were able to decolorize and detoxify the dye more efficiently than the monocultures. The noticeable increase in laccase activity along with the more efficient decolorization and detoxification of MG by co-cultures of G.lucidum and T.versicolor in SSF makes this system a viable strategy for large scale application of white-rot cultures in bioremediation. © 2015 Elsevier Ltd. Fil:Castiglia, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v104_n_p238_Kuhar http://hdl.handle.net/20.500.12110/paper_09648305_v104_n_p238_Kuhar |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Co-cultivation Laccase Malachite green White-rot fungi Bioremediation Biotechnology Carbonate minerals Cellulose Detoxification Fermentation Fungi Lignin Wood Cellulose and hemicellulose Co-cultivation Laccases Large-scale applications Malachite green Solid-state fermentation Trametes versicolor White rot fungi Enzymes bioremediation color enzyme activity fermentation fungal disease fungus malachite monoculture optimization Fermentation Laccase Production Fungi Ganoderma lucidum Trametes versicolor |
| spellingShingle |
Co-cultivation Laccase Malachite green White-rot fungi Bioremediation Biotechnology Carbonate minerals Cellulose Detoxification Fermentation Fungi Lignin Wood Cellulose and hemicellulose Co-cultivation Laccases Large-scale applications Malachite green Solid-state fermentation Trametes versicolor White rot fungi Enzymes bioremediation color enzyme activity fermentation fungal disease fungus malachite monoculture optimization Fermentation Laccase Production Fungi Ganoderma lucidum Trametes versicolor Castiglia, Valeria Carolina Levin, Laura Noemi Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation |
| topic_facet |
Co-cultivation Laccase Malachite green White-rot fungi Bioremediation Biotechnology Carbonate minerals Cellulose Detoxification Fermentation Fungi Lignin Wood Cellulose and hemicellulose Co-cultivation Laccases Large-scale applications Malachite green Solid-state fermentation Trametes versicolor White rot fungi Enzymes bioremediation color enzyme activity fermentation fungal disease fungus malachite monoculture optimization Fermentation Laccase Production Fungi Ganoderma lucidum Trametes versicolor |
| description |
Optimization of laccase production in white-rot fungi has been extensively studied. Metallic and aromatic compounds have been found to enhance enzyme production, but the development of bioremediation as an application field of this enzyme requires clean technologies. In this work, co-cultivation of Ganoderma lucidum and Trametes versicolor was performed, showing remarkable enhancement of laccase activity. Dual cultures were assayed for malachite green degradation (MG) in solid state fermentation (SSF) using a sawdust-based medium. The time for achieving complete decolorization of MG in co-cultivation was markedly shorter than that observed in monocultures. Dual-species treatment did not differ in wood dry weight loss and lignin, cellulose and hemicellulose degradation, compared to monocultures; but the selectivity index (lignin loss/cellulose loss) of dual cultures was markedly higher than those attained by monocultures. Moreover, a modified isoenzymatic laccase pattern was observed, showing one isoenzyme that was absent in monocultures. Dual cultures were able to decolorize and detoxify the dye more efficiently than the monocultures. The noticeable increase in laccase activity along with the more efficient decolorization and detoxification of MG by co-cultures of G.lucidum and T.versicolor in SSF makes this system a viable strategy for large scale application of white-rot cultures in bioremediation. © 2015 Elsevier Ltd. |
| author |
Castiglia, Valeria Carolina Levin, Laura Noemi |
| author_facet |
Castiglia, Valeria Carolina Levin, Laura Noemi |
| author_sort |
Castiglia, Valeria Carolina |
| title |
Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation |
| title_short |
Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation |
| title_full |
Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation |
| title_fullStr |
Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation |
| title_full_unstemmed |
Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation |
| title_sort |
enhancement of laccase production and malachite green decolorization by co-culturing ganoderma lucidum and trametes versicolor in solid-state fermentation |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v104_n_p238_Kuhar http://hdl.handle.net/20.500.12110/paper_09648305_v104_n_p238_Kuhar |
| work_keys_str_mv |
AT castigliavaleriacarolina enhancementoflaccaseproductionandmalachitegreendecolorizationbycoculturingganodermalucidumandtrametesversicolorinsolidstatefermentation AT levinlauranoemi enhancementoflaccaseproductionandmalachitegreendecolorizationbycoculturingganodermalucidumandtrametesversicolorinsolidstatefermentation |
| _version_ |
1768545152973930496 |