Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields
Inactivation of three Saccharomyces cerevisiae strains by PEF treatment, at different growth phases and initial inoculum sizes was investigated. The conditions of the PEF treatment were: electric field level of 8-11 kV/cm, 3 pulses, 50 μF capacitance or 12.5 kV/cm, 40 pulses, 1 μF capacitance. The i...
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paper:paper_09639969_v37_n8_p793_Molinari2023-06-08T15:58:22Z Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields Pilosof, Ana María Renata Jagus de Kanterewicz, Rosa Juana Concentration Pulsed electric field Yeast Capacitance Electric fields Fruit juices Yeast growth phases Inoculum size Food products Saccharomyces Saccharomyces cerevisiae Inactivation of three Saccharomyces cerevisiae strains by PEF treatment, at different growth phases and initial inoculum sizes was investigated. The conditions of the PEF treatment were: electric field level of 8-11 kV/cm, 3 pulses, 50 μF capacitance or 12.5 kV/cm, 40 pulses, 1 μF capacitance. The inactivation of yeasts ranged from 2 to 5.8 log cycles when the inoculum size increased from 5×105 to 1×1011 CFU/ml, respectively. In all the experiments, the reduction of yeasts population was linearly enhanced by increasing the inoculum size, independently of the growth phase. Different experiments were designed to explain the observed behavior. By increasing yeasts concentration the probability of formation of pearl chains would increase. As a consequence of the large volume of these "equivalent cells" the PEF treatment efficiency would increase. © 2004 Elsevier Ltd. All rights reserved. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jagus, R.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09639969_v37_n8_p793_Molinari http://hdl.handle.net/20.500.12110/paper_09639969_v37_n8_p793_Molinari |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Concentration Pulsed electric field Yeast Capacitance Electric fields Fruit juices Yeast growth phases Inoculum size Food products Saccharomyces Saccharomyces cerevisiae |
| spellingShingle |
Concentration Pulsed electric field Yeast Capacitance Electric fields Fruit juices Yeast growth phases Inoculum size Food products Saccharomyces Saccharomyces cerevisiae Pilosof, Ana María Renata Jagus de Kanterewicz, Rosa Juana Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields |
| topic_facet |
Concentration Pulsed electric field Yeast Capacitance Electric fields Fruit juices Yeast growth phases Inoculum size Food products Saccharomyces Saccharomyces cerevisiae |
| description |
Inactivation of three Saccharomyces cerevisiae strains by PEF treatment, at different growth phases and initial inoculum sizes was investigated. The conditions of the PEF treatment were: electric field level of 8-11 kV/cm, 3 pulses, 50 μF capacitance or 12.5 kV/cm, 40 pulses, 1 μF capacitance. The inactivation of yeasts ranged from 2 to 5.8 log cycles when the inoculum size increased from 5×105 to 1×1011 CFU/ml, respectively. In all the experiments, the reduction of yeasts population was linearly enhanced by increasing the inoculum size, independently of the growth phase. Different experiments were designed to explain the observed behavior. By increasing yeasts concentration the probability of formation of pearl chains would increase. As a consequence of the large volume of these "equivalent cells" the PEF treatment efficiency would increase. © 2004 Elsevier Ltd. All rights reserved. |
| author |
Pilosof, Ana María Renata Jagus de Kanterewicz, Rosa Juana |
| author_facet |
Pilosof, Ana María Renata Jagus de Kanterewicz, Rosa Juana |
| author_sort |
Pilosof, Ana María Renata |
| title |
Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields |
| title_short |
Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields |
| title_full |
Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields |
| title_fullStr |
Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields |
| title_full_unstemmed |
Effect of growth phase and inoculum size on the inactivation of Saccharomyces cerevisiae in fruit juices, by pulsed electric fields |
| title_sort |
effect of growth phase and inoculum size on the inactivation of saccharomyces cerevisiae in fruit juices, by pulsed electric fields |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09639969_v37_n8_p793_Molinari http://hdl.handle.net/20.500.12110/paper_09639969_v37_n8_p793_Molinari |
| work_keys_str_mv |
AT pilosofanamariarenata effectofgrowthphaseandinoculumsizeontheinactivationofsaccharomycescerevisiaeinfruitjuicesbypulsedelectricfields AT jagusdekanterewiczrosajuana effectofgrowthphaseandinoculumsizeontheinactivationofsaccharomycescerevisiaeinfruitjuicesbypulsedelectricfields |
| _version_ |
1768545199495053312 |