Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis
The synthesis of modified nucleosides has received a great deal of attention due to their applications as antiviral and antitumoral agents. Among the different synthetic strategies, microbial transglycosylation has already shown to provide successful results. In the present work, we analyse the use...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09636048_v6_n4_p376_Trelles http://hdl.handle.net/20.500.12110/paper_09636048_v6_n4_p376_Trelles |
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paper:paper_09636048_v6_n4_p376_Trelles2023-06-08T15:58:11Z Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Citrobacter amalonaticus Polyacrylamide Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism catalysis Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms) Citrobacter Citrobacter amalonaticus The synthesis of modified nucleosides has received a great deal of attention due to their applications as antiviral and antitumoral agents. Among the different synthetic strategies, microbial transglycosylation has already shown to provide successful results. In the present work, we analyse the use of Citrobacter amalonaticus CECT 863 cells - free or immobilised - in the synthesis of some modified nucleosides. The main characteristics of the immobilised biocatalysts such as type and concentration of polymer, mechanical and storage stability and reuse were assessed. From these studies, polyacrylamide was selected as the best support based on its performance and potential industrial applications. In particular, it can be reused more than 50 times without significant loss of activity. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09636048_v6_n4_p376_Trelles http://hdl.handle.net/20.500.12110/paper_09636048_v6_n4_p376_Trelles |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Citrobacter amalonaticus Polyacrylamide Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism catalysis Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms) Citrobacter Citrobacter amalonaticus |
| spellingShingle |
Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Citrobacter amalonaticus Polyacrylamide Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism catalysis Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms) Citrobacter Citrobacter amalonaticus Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis |
| topic_facet |
Cell immobilisation Citrobacter amalonaticus Microbial transglycosylation Modified nucleosides Polyacrylamide Concentration (process) Performance Polymers Synthesis (chemical) Antitumoral agents Antiviral agents Citrobacter amalonaticus Polyacrylamide Biocatalysts nucleoside polyacrylamide catalysis article bacterial metabolism catalysis Citrobacter citrobacter amalonaticus glycosylation immobilized cell nonhuman nucleotide metabolism Bacteria (microorganisms) Citrobacter Citrobacter amalonaticus |
| description |
The synthesis of modified nucleosides has received a great deal of attention due to their applications as antiviral and antitumoral agents. Among the different synthetic strategies, microbial transglycosylation has already shown to provide successful results. In the present work, we analyse the use of Citrobacter amalonaticus CECT 863 cells - free or immobilised - in the synthesis of some modified nucleosides. The main characteristics of the immobilised biocatalysts such as type and concentration of polymer, mechanical and storage stability and reuse were assessed. From these studies, polyacrylamide was selected as the best support based on its performance and potential industrial applications. In particular, it can be reused more than 50 times without significant loss of activity. |
| title |
Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis |
| title_short |
Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis |
| title_full |
Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis |
| title_fullStr |
Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis |
| title_full_unstemmed |
Free and immobilised Citrobacter amalonaticus CECT 863 as a biocatalyst for nucleoside synthesis |
| title_sort |
free and immobilised citrobacter amalonaticus cect 863 as a biocatalyst for nucleoside synthesis |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09636048_v6_n4_p376_Trelles http://hdl.handle.net/20.500.12110/paper_09636048_v6_n4_p376_Trelles |
| _version_ |
1768542091161370624 |