Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America
Archaeological maize specimens from Andean sites of southern South America, dating from 400 to 1400 years before present, were tested for the presence of ancient DNA and three microsatellite loci were typed in the specimens that gave positive results. Genotypes were also obtained for 146 individuals...
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2007
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09628452_v274_n1609_p545_Lia http://hdl.handle.net/20.500.12110/paper_09628452_v274_n1609_p545_Lia |
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paper:paper_09628452_v274_n1609_p545_Lia2023-06-08T15:58:05Z Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America Lia, Verónica Viviana Confalonieri, Viviana Andrea Poggio, Lidia Ancient DNA Landraces Maize Microsatellites South America microsatellite DNA microsatellite DNA agricultural history archaeological evidence cultivation DNA genetic analysis genetic marker genotype maize agriculture allelism Argentina article DNA fingerprinting DNA sequence gene locus gene pool genetic variability genotype history landrace maize molecular cloning nonhuman nucleotide sequence priority journal sequence analysis South America tillage classification crop genetic polymorphism genetics geography phylogeny Andes South America Zea mays Crops, Agricultural Genotype Geography Microsatellite Repeats Phylogeny Polymorphism, Genetic Sequence Analysis, DNA South America Zea mays Archaeological maize specimens from Andean sites of southern South America, dating from 400 to 1400 years before present, were tested for the presence of ancient DNA and three microsatellite loci were typed in the specimens that gave positive results. Genotypes were also obtained for 146 individuals corresponding to modern landraces currently cultivated in the same areas and for 21 plants from Argentinian lowland races. Sequence analysis of cloned ancient DNA products revealed a high incidence of substitutions appearing in only one clone, with transitions prevalent. In the archaeological specimens, there was no evidence of polymorphism at any one of the three microsatellite loci: each exhibited a single allelic variant, identical to the most frequent allele found in contemporary populations belonging to races Amarillo Chico, Amarillo Grande, Blanco and Altiplano. Affiliation between ancient specimens and a set of races from the Andean complex was further supported by assignment tests. The striking genetic uniformity displayed by the ancient specimens and their close relationship with the Andean complex suggest that the latter gene pool has predominated in the western regions of southern South America for at least the past 1400 years. The results support hypotheses suggesting that maize cultivation initially spread into South America via a highland route, rather than through the lowlands. © 2006 The Royal Society. Fil:Lia, V.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Confalonieri, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09628452_v274_n1609_p545_Lia http://hdl.handle.net/20.500.12110/paper_09628452_v274_n1609_p545_Lia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ancient DNA Landraces Maize Microsatellites South America microsatellite DNA microsatellite DNA agricultural history archaeological evidence cultivation DNA genetic analysis genetic marker genotype maize agriculture allelism Argentina article DNA fingerprinting DNA sequence gene locus gene pool genetic variability genotype history landrace maize molecular cloning nonhuman nucleotide sequence priority journal sequence analysis South America tillage classification crop genetic polymorphism genetics geography phylogeny Andes South America Zea mays Crops, Agricultural Genotype Geography Microsatellite Repeats Phylogeny Polymorphism, Genetic Sequence Analysis, DNA South America Zea mays |
| spellingShingle |
Ancient DNA Landraces Maize Microsatellites South America microsatellite DNA microsatellite DNA agricultural history archaeological evidence cultivation DNA genetic analysis genetic marker genotype maize agriculture allelism Argentina article DNA fingerprinting DNA sequence gene locus gene pool genetic variability genotype history landrace maize molecular cloning nonhuman nucleotide sequence priority journal sequence analysis South America tillage classification crop genetic polymorphism genetics geography phylogeny Andes South America Zea mays Crops, Agricultural Genotype Geography Microsatellite Repeats Phylogeny Polymorphism, Genetic Sequence Analysis, DNA South America Zea mays Lia, Verónica Viviana Confalonieri, Viviana Andrea Poggio, Lidia Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America |
| topic_facet |
Ancient DNA Landraces Maize Microsatellites South America microsatellite DNA microsatellite DNA agricultural history archaeological evidence cultivation DNA genetic analysis genetic marker genotype maize agriculture allelism Argentina article DNA fingerprinting DNA sequence gene locus gene pool genetic variability genotype history landrace maize molecular cloning nonhuman nucleotide sequence priority journal sequence analysis South America tillage classification crop genetic polymorphism genetics geography phylogeny Andes South America Zea mays Crops, Agricultural Genotype Geography Microsatellite Repeats Phylogeny Polymorphism, Genetic Sequence Analysis, DNA South America Zea mays |
| description |
Archaeological maize specimens from Andean sites of southern South America, dating from 400 to 1400 years before present, were tested for the presence of ancient DNA and three microsatellite loci were typed in the specimens that gave positive results. Genotypes were also obtained for 146 individuals corresponding to modern landraces currently cultivated in the same areas and for 21 plants from Argentinian lowland races. Sequence analysis of cloned ancient DNA products revealed a high incidence of substitutions appearing in only one clone, with transitions prevalent. In the archaeological specimens, there was no evidence of polymorphism at any one of the three microsatellite loci: each exhibited a single allelic variant, identical to the most frequent allele found in contemporary populations belonging to races Amarillo Chico, Amarillo Grande, Blanco and Altiplano. Affiliation between ancient specimens and a set of races from the Andean complex was further supported by assignment tests. The striking genetic uniformity displayed by the ancient specimens and their close relationship with the Andean complex suggest that the latter gene pool has predominated in the western regions of southern South America for at least the past 1400 years. The results support hypotheses suggesting that maize cultivation initially spread into South America via a highland route, rather than through the lowlands. © 2006 The Royal Society. |
| author |
Lia, Verónica Viviana Confalonieri, Viviana Andrea Poggio, Lidia |
| author_facet |
Lia, Verónica Viviana Confalonieri, Viviana Andrea Poggio, Lidia |
| author_sort |
Lia, Verónica Viviana |
| title |
Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America |
| title_short |
Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America |
| title_full |
Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America |
| title_fullStr |
Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America |
| title_full_unstemmed |
Microsatellite typing of ancient maize: Insights into the history of agriculture in southern South America |
| title_sort |
microsatellite typing of ancient maize: insights into the history of agriculture in southern south america |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09628452_v274_n1609_p545_Lia http://hdl.handle.net/20.500.12110/paper_09628452_v274_n1609_p545_Lia |
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