Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila

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High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophil...

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Detalles Bibliográficos
Autores principales: Padro, Julian, Soto, Ignacio M., Dopazo, Hernán Javier, Hasson, Esteban Ruben
Publicado: 2016
Materias:
Alkaloids
Environment adaptation
Mescaline
Plasticity
RNA-Seq
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09621083_v_n_p_DePanis
http://hdl.handle.net/20.500.12110/paper_09621083_v_n_p_DePanis
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09621083_v_n_p_DePanis
record_format dspace
spelling paper:paper_09621083_v_n_p_DePanis2023-06-08T15:58:02Z Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila Padro, Julian Soto, Ignacio M. Dopazo, Hernán Javier Hasson, Esteban Ruben Alkaloids Environment adaptation Mescaline Plasticity RNA-Seq High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation-reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments. © 2016 John Wiley & Sons Ltd. Fil:Padró, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Soto, I.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dopazo, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hasson, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09621083_v_n_p_DePanis http://hdl.handle.net/20.500.12110/paper_09621083_v_n_p_DePanis
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Alkaloids
Environment adaptation
Mescaline
Plasticity
RNA-Seq
spellingShingle Alkaloids
Environment adaptation
Mescaline
Plasticity
RNA-Seq
Padro, Julian
Soto, Ignacio M.
Dopazo, Hernán Javier
Hasson, Esteban Ruben
Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
topic_facet Alkaloids
Environment adaptation
Mescaline
Plasticity
RNA-Seq
description High-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. Nevertheless, much remains to be understood about the genetic basis of host plant adaptation. Here, we investigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation-reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments. © 2016 John Wiley & Sons Ltd.
author Padro, Julian
Soto, Ignacio M.
Dopazo, Hernán Javier
Hasson, Esteban Ruben
author_facet Padro, Julian
Soto, Ignacio M.
Dopazo, Hernán Javier
Hasson, Esteban Ruben
author_sort Padro, Julian
title Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_short Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_full Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_fullStr Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_full_unstemmed Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila
title_sort transcriptome modulation during host shift is driven by secondary metabolites in desert drosophila
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09621083_v_n_p_DePanis
http://hdl.handle.net/20.500.12110/paper_09621083_v_n_p_DePanis
work_keys_str_mv AT padrojulian transcriptomemodulationduringhostshiftisdrivenbysecondarymetabolitesindesertdrosophila
AT sotoignaciom transcriptomemodulationduringhostshiftisdrivenbysecondarymetabolitesindesertdrosophila
AT dopazohernanjavier transcriptomemodulationduringhostshiftisdrivenbysecondarymetabolitesindesertdrosophila
AT hassonestebanruben transcriptomemodulationduringhostshiftisdrivenbysecondarymetabolitesindesertdrosophila
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