Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster
Knockdown resistance to high temperature is an ecologically important trait in small insects. A composite interval mapping was performed on the two major autosomes of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting knockdown resistance to high temperature (KRHT). Two dr...
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paper:paper_09621083_v13_n11_p3585_Norry2023-06-08T15:58:00Z Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster Norry, Fabian Marcelo Dominance Drosophila Heat-knockdown resistance Heat-shock proteins Heat-stress resistance QTL Thermal adaptation microsatellite DNA animal article chromosome map cross breeding DNA sequence Drosophila melanogaster genetic linkage genetic marker genetics heat male molecular genetics physiology quantitative trait quantitative trait locus X chromosome Animals Chromosome Mapping Crosses, Genetic Drosophila melanogaster Genetic Markers Heat Lod Score Male Microsatellite Repeats Molecular Sequence Data Quantitative Trait Loci Quantitative Trait, Heritable Sequence Analysis, DNA X Chromosome Drosophila melanogaster Hexapoda Insecta Melanogaster Knockdown resistance to high temperature is an ecologically important trait in small insects. A composite interval mapping was performed on the two major autosomes of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting knockdown resistance to high temperature (KRHT). Two dramatically divergent lines from geographically different thermal environments were artificially selected on KRHT. These lines were crossed to produce two backcross (BC) populations. Each BC was analysed for 200 males with 18 marker loci on chromosomes 2 and 3. Three X-linked markers were used to test for X-linked QTL in an exploratory way. The largest estimate of autosome additive effects was found in the pericentromeric region of chromosome 2, accounting for 19.26% (BC to the low line) and 29.15% (BC to the high line) of the phenotypic variance in BC populations, but it could represent multiple closely linked QTL. Complete dominance was apparent for three QTL on chromosome 3, where heat-shock genes are concentrated. Exploratory analysis of chromosome X indicated a substantial contribution of this chromosome to KRHT. The results show that a large-effect QTL with dominant gene action maps on the right arm of chromosome 3. Further, the results confirm that QTL for heat resistance are not limited to chromosome 3. Fil:Norry, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09621083_v13_n11_p3585_Norry http://hdl.handle.net/20.500.12110/paper_09621083_v13_n11_p3585_Norry |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dominance Drosophila Heat-knockdown resistance Heat-shock proteins Heat-stress resistance QTL Thermal adaptation microsatellite DNA animal article chromosome map cross breeding DNA sequence Drosophila melanogaster genetic linkage genetic marker genetics heat male molecular genetics physiology quantitative trait quantitative trait locus X chromosome Animals Chromosome Mapping Crosses, Genetic Drosophila melanogaster Genetic Markers Heat Lod Score Male Microsatellite Repeats Molecular Sequence Data Quantitative Trait Loci Quantitative Trait, Heritable Sequence Analysis, DNA X Chromosome Drosophila melanogaster Hexapoda Insecta Melanogaster |
| spellingShingle |
Dominance Drosophila Heat-knockdown resistance Heat-shock proteins Heat-stress resistance QTL Thermal adaptation microsatellite DNA animal article chromosome map cross breeding DNA sequence Drosophila melanogaster genetic linkage genetic marker genetics heat male molecular genetics physiology quantitative trait quantitative trait locus X chromosome Animals Chromosome Mapping Crosses, Genetic Drosophila melanogaster Genetic Markers Heat Lod Score Male Microsatellite Repeats Molecular Sequence Data Quantitative Trait Loci Quantitative Trait, Heritable Sequence Analysis, DNA X Chromosome Drosophila melanogaster Hexapoda Insecta Melanogaster Norry, Fabian Marcelo Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster |
| topic_facet |
Dominance Drosophila Heat-knockdown resistance Heat-shock proteins Heat-stress resistance QTL Thermal adaptation microsatellite DNA animal article chromosome map cross breeding DNA sequence Drosophila melanogaster genetic linkage genetic marker genetics heat male molecular genetics physiology quantitative trait quantitative trait locus X chromosome Animals Chromosome Mapping Crosses, Genetic Drosophila melanogaster Genetic Markers Heat Lod Score Male Microsatellite Repeats Molecular Sequence Data Quantitative Trait Loci Quantitative Trait, Heritable Sequence Analysis, DNA X Chromosome Drosophila melanogaster Hexapoda Insecta Melanogaster |
| description |
Knockdown resistance to high temperature is an ecologically important trait in small insects. A composite interval mapping was performed on the two major autosomes of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting knockdown resistance to high temperature (KRHT). Two dramatically divergent lines from geographically different thermal environments were artificially selected on KRHT. These lines were crossed to produce two backcross (BC) populations. Each BC was analysed for 200 males with 18 marker loci on chromosomes 2 and 3. Three X-linked markers were used to test for X-linked QTL in an exploratory way. The largest estimate of autosome additive effects was found in the pericentromeric region of chromosome 2, accounting for 19.26% (BC to the low line) and 29.15% (BC to the high line) of the phenotypic variance in BC populations, but it could represent multiple closely linked QTL. Complete dominance was apparent for three QTL on chromosome 3, where heat-shock genes are concentrated. Exploratory analysis of chromosome X indicated a substantial contribution of this chromosome to KRHT. The results show that a large-effect QTL with dominant gene action maps on the right arm of chromosome 3. Further, the results confirm that QTL for heat resistance are not limited to chromosome 3. |
| author |
Norry, Fabian Marcelo |
| author_facet |
Norry, Fabian Marcelo |
| author_sort |
Norry, Fabian Marcelo |
| title |
Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster |
| title_short |
Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster |
| title_full |
Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster |
| title_fullStr |
Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster |
| title_full_unstemmed |
Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster |
| title_sort |
quantitative trait loci affecting knockdown resistance to high temperature in drosophila melanogaster |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09621083_v13_n11_p3585_Norry http://hdl.handle.net/20.500.12110/paper_09621083_v13_n11_p3585_Norry |
| work_keys_str_mv |
AT norryfabianmarcelo quantitativetraitlociaffectingknockdownresistancetohightemperatureindrosophilamelanogaster |
| _version_ |
1768542186553475072 |