2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor

A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthe...

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Publicado:	2010
Materias:	2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v20_n9_p2806_Pontiggia http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n9_p2806_Pontiggia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0960894X_v20_n9_p2806_Pontiggia
record_format	dspace
spelling	paper:paper_0960894X_v20_n9_p2806_Pontiggia2023-06-08T15:57:51Z 2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor 2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor α protein (ER-α), and transfection experiments in MCF-7 cell line are also presented. © 2010 Elsevier Ltd. All rights reserved. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v20_n9_p2806_Pontiggia http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n9_p2806_Pontiggia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine
spellingShingle	2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine 2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor
topic_facet	2′-C-methyluridine Estrogen receptor alpha Modified ribozyme estrogen receptor alpha hammerhead ribozyme hormone receptor blocking agent hydroxyl group phosphoramidous acid derivative article cell strain MCF 7 chemical modification controlled study drug stability drug synthesis enzyme active site genetic transfection receptor down regulation Cell Line, Tumor Humans Oligonucleotides Receptors, Estrogen RNA, Catalytic RNA, Messenger Uridine
description	A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor α protein (ER-α), and transfection experiments in MCF-7 cell line are also presented. © 2010 Elsevier Ltd. All rights reserved.
title	2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor
title_short	2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor
title_full	2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor
title_fullStr	2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor
title_full_unstemmed	2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor
title_sort	2-c-methyluridine modified hammerhead ribozyme against the estrogen receptor
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v20_n9_p2806_Pontiggia http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n9_p2806_Pontiggia
_version_	1768544786344574976

2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor

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