Hyaluronan oligosaccharides induce cell death through PI3-K/Akt pathway independently of NF-κB transcription factor
Several studies indicate that hyaluronan oligosaccharides (oHA) are able to modulate growth and cell survival in solid tumors; however, no studies have been undertaken to analyze the effect of oHA on T-lymphoid disorders. In this work we showed that oHA were able to induce apoptosis in lymphoma cell...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v16_n5_p359_Alaniz http://hdl.handle.net/20.500.12110/paper_09596658_v16_n5_p359_Alaniz |
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paper:paper_09596658_v16_n5_p359_Alaniz2023-06-08T15:57:02Z Hyaluronan oligosaccharides induce cell death through PI3-K/Akt pathway independently of NF-κB transcription factor Gallo, Carola Agustí, Rosalía Akt Apoptosis HPAEC-PAD Hyaluronan oligomers NF-κB P13-K hyaluronic acid I kappa B alpha immunoglobulin enhancer binding protein oligosaccharide phosphatidylinositol 3 kinase phosphatidylinositol 3,4,5 trisphosphate protein kinase B wortmannin amperometry animal cell anion exchange chromatography apoptosis article cancer cell culture cell death cell function cell survival controlled study enzyme activity enzyme inhibition enzyme synthesis hypothesis inhibition kinetics lymphoma mouse nonhuman pH priority journal protein phosphorylation signal transduction 1-Phosphatidylinositol 3-Kinase Animals Apoptosis Cell Death Cell Line, Tumor Dose-Response Relationship, Drug Hyaluronic Acid Lymphoma Mice Molecular Weight NF-kappa B Proto-Oncogene Proteins c-akt Signal Transduction Animalia Several studies indicate that hyaluronan oligosaccharides (oHA) are able to modulate growth and cell survival in solid tumors; however, no studies have been undertaken to analyze the effect of oHA on T-lymphoid disorders. In this work we showed that oHA were able to induce apoptosis in lymphoma cell lines. Since PI3-K/Akt and nuclear factor-κB (NF-κB) are major factors involved in cell survival and anti-apoptotic pathways in lymphoma cells, we hypothesized that oHA could induce apoptosis through inhibition of these pathways. oHA were identified by a method which allows characterization of length using a high pH anion exchange chromatography with pulse amperometric detection (HPAEC-PAD). oHA inhibited PIP3 production (principal product of PI3-K activity) and reduced Akt phosphorylation levels, similarly to the specific inhibitor wortmannin. However, treatment with either oHA or wortmannin failed to inhibit constitutive NF-κB activity and modulate IκBα protein levels, suggesting that PI3-K and NF-κB signaling pathways are not related in the cell lines used. Cell behavior differed using native hyaluronan (HA), which induced PIP3 production, Akt phosphorylation, and NF-κB activation, although not related with cell survival since treatment with native HA showed no effect on apoptosis. Our results suggest that oHA induce apoptosis by suppression of PI3-K/Akt cell survival pathway without involving NF-κB activation, through a mechanism that differs from the one mediated by native HA. © 2006 Oxford University Press. Fil:Gallo-Rodriguez, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Agusti, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v16_n5_p359_Alaniz http://hdl.handle.net/20.500.12110/paper_09596658_v16_n5_p359_Alaniz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Akt Apoptosis HPAEC-PAD Hyaluronan oligomers NF-κB P13-K hyaluronic acid I kappa B alpha immunoglobulin enhancer binding protein oligosaccharide phosphatidylinositol 3 kinase phosphatidylinositol 3,4,5 trisphosphate protein kinase B wortmannin amperometry animal cell anion exchange chromatography apoptosis article cancer cell culture cell death cell function cell survival controlled study enzyme activity enzyme inhibition enzyme synthesis hypothesis inhibition kinetics lymphoma mouse nonhuman pH priority journal protein phosphorylation signal transduction 1-Phosphatidylinositol 3-Kinase Animals Apoptosis Cell Death Cell Line, Tumor Dose-Response Relationship, Drug Hyaluronic Acid Lymphoma Mice Molecular Weight NF-kappa B Proto-Oncogene Proteins c-akt Signal Transduction Animalia |
| spellingShingle |
Akt Apoptosis HPAEC-PAD Hyaluronan oligomers NF-κB P13-K hyaluronic acid I kappa B alpha immunoglobulin enhancer binding protein oligosaccharide phosphatidylinositol 3 kinase phosphatidylinositol 3,4,5 trisphosphate protein kinase B wortmannin amperometry animal cell anion exchange chromatography apoptosis article cancer cell culture cell death cell function cell survival controlled study enzyme activity enzyme inhibition enzyme synthesis hypothesis inhibition kinetics lymphoma mouse nonhuman pH priority journal protein phosphorylation signal transduction 1-Phosphatidylinositol 3-Kinase Animals Apoptosis Cell Death Cell Line, Tumor Dose-Response Relationship, Drug Hyaluronic Acid Lymphoma Mice Molecular Weight NF-kappa B Proto-Oncogene Proteins c-akt Signal Transduction Animalia Gallo, Carola Agustí, Rosalía Hyaluronan oligosaccharides induce cell death through PI3-K/Akt pathway independently of NF-κB transcription factor |
| topic_facet |
Akt Apoptosis HPAEC-PAD Hyaluronan oligomers NF-κB P13-K hyaluronic acid I kappa B alpha immunoglobulin enhancer binding protein oligosaccharide phosphatidylinositol 3 kinase phosphatidylinositol 3,4,5 trisphosphate protein kinase B wortmannin amperometry animal cell anion exchange chromatography apoptosis article cancer cell culture cell death cell function cell survival controlled study enzyme activity enzyme inhibition enzyme synthesis hypothesis inhibition kinetics lymphoma mouse nonhuman pH priority journal protein phosphorylation signal transduction 1-Phosphatidylinositol 3-Kinase Animals Apoptosis Cell Death Cell Line, Tumor Dose-Response Relationship, Drug Hyaluronic Acid Lymphoma Mice Molecular Weight NF-kappa B Proto-Oncogene Proteins c-akt Signal Transduction Animalia |
| description |
Several studies indicate that hyaluronan oligosaccharides (oHA) are able to modulate growth and cell survival in solid tumors; however, no studies have been undertaken to analyze the effect of oHA on T-lymphoid disorders. In this work we showed that oHA were able to induce apoptosis in lymphoma cell lines. Since PI3-K/Akt and nuclear factor-κB (NF-κB) are major factors involved in cell survival and anti-apoptotic pathways in lymphoma cells, we hypothesized that oHA could induce apoptosis through inhibition of these pathways. oHA were identified by a method which allows characterization of length using a high pH anion exchange chromatography with pulse amperometric detection (HPAEC-PAD). oHA inhibited PIP3 production (principal product of PI3-K activity) and reduced Akt phosphorylation levels, similarly to the specific inhibitor wortmannin. However, treatment with either oHA or wortmannin failed to inhibit constitutive NF-κB activity and modulate IκBα protein levels, suggesting that PI3-K and NF-κB signaling pathways are not related in the cell lines used. Cell behavior differed using native hyaluronan (HA), which induced PIP3 production, Akt phosphorylation, and NF-κB activation, although not related with cell survival since treatment with native HA showed no effect on apoptosis. Our results suggest that oHA induce apoptosis by suppression of PI3-K/Akt cell survival pathway without involving NF-κB activation, through a mechanism that differs from the one mediated by native HA. © 2006 Oxford University Press. |
| author |
Gallo, Carola Agustí, Rosalía |
| author_facet |
Gallo, Carola Agustí, Rosalía |
| author_sort |
Gallo, Carola |
| title |
Hyaluronan oligosaccharides induce cell death through PI3-K/Akt pathway independently of NF-κB transcription factor |
| title_short |
Hyaluronan oligosaccharides induce cell death through PI3-K/Akt pathway independently of NF-κB transcription factor |
| title_full |
Hyaluronan oligosaccharides induce cell death through PI3-K/Akt pathway independently of NF-κB transcription factor |
| title_fullStr |
Hyaluronan oligosaccharides induce cell death through PI3-K/Akt pathway independently of NF-κB transcription factor |
| title_full_unstemmed |
Hyaluronan oligosaccharides induce cell death through PI3-K/Akt pathway independently of NF-κB transcription factor |
| title_sort |
hyaluronan oligosaccharides induce cell death through pi3-k/akt pathway independently of nf-κb transcription factor |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v16_n5_p359_Alaniz http://hdl.handle.net/20.500.12110/paper_09596658_v16_n5_p359_Alaniz |
| work_keys_str_mv |
AT gallocarola hyaluronanoligosaccharidesinducecelldeaththroughpi3kaktpathwayindependentlyofnfkbtranscriptionfactor AT agustirosalia hyaluronanoligosaccharidesinducecelldeaththroughpi3kaktpathwayindependentlyofnfkbtranscriptionfactor |
| _version_ |
1768543333785796608 |