BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells

The effects of the membrane permeant Ca2+ chelator BAPTA-AM on voltage-gated Na+, Ca2+, K+ (I(Na), I(Ca), I(K), respectively) and Ca2+-activated K+ (I(KCa)) currents in cultured bovine chromaffin cells were investigated using the whole-cell patch-clamp technique. Superfusion with BAPTA-AM (50 μM) in...

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Detalles Bibliográficos
Publicado: 1998
Materias:
BAPTA
BAPTA-AM
Extracellular block
Open channel block
Potassium current block
animal cell
article
calcium transport
cattle
channel gating
chromaffin cell
nonhuman
patch clamp
potassium current
priority journal
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v9_n15_p3403_Urbano
http://hdl.handle.net/20.500.12110/paper_09594965_v9_n15_p3403_Urbano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09594965_v9_n15_p3403_Urbano
record_format dspace
spelling paper:paper_09594965_v9_n15_p3403_Urbano2023-06-08T15:57:01Z BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells BAPTA BAPTA-AM Extracellular block Open channel block Potassium current block animal cell article calcium transport cattle channel gating chromaffin cell nonhuman patch clamp potassium current priority journal The effects of the membrane permeant Ca2+ chelator BAPTA-AM on voltage-gated Na+, Ca2+, K+ (I(Na), I(Ca), I(K), respectively) and Ca2+-activated K+ (I(KCa)) currents in cultured bovine chromaffin cells were investigated using the whole-cell patch-clamp technique. Superfusion with BAPTA-AM (50 μM) induced a rapid (< 60 s) and reversible block of both I(KCa) and I(K) (~50%), without affecting either I(Ca) or I(Na). Preincubation with BAPTA-AM (50 μM, 30 min) or cell loading with the non- permeable active form of BAPTA (10 mM in the pipette solution) permanently blocked I(KCa). BAPTA-AM superfusion (50 μM) also blocked I(K) (~53%) after BAPTA-loading or BAPTA-AM preincubation. In conclusion, we show a fast and reversible block of I(KCa) and I(K) by BAPTA-AM, acting directly on K+ channels before it operates as a Ca2+ chelator, in cultured bovine chromaffin cells. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v9_n15_p3403_Urbano http://hdl.handle.net/20.500.12110/paper_09594965_v9_n15_p3403_Urbano
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic BAPTA
BAPTA-AM
Extracellular block
Open channel block
Potassium current block
animal cell
article
calcium transport
cattle
channel gating
chromaffin cell
nonhuman
patch clamp
potassium current
priority journal
spellingShingle BAPTA
BAPTA-AM
Extracellular block
Open channel block
Potassium current block
animal cell
article
calcium transport
cattle
channel gating
chromaffin cell
nonhuman
patch clamp
potassium current
priority journal
BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells
topic_facet BAPTA
BAPTA-AM
Extracellular block
Open channel block
Potassium current block
animal cell
article
calcium transport
cattle
channel gating
chromaffin cell
nonhuman
patch clamp
potassium current
priority journal
description The effects of the membrane permeant Ca2+ chelator BAPTA-AM on voltage-gated Na+, Ca2+, K+ (I(Na), I(Ca), I(K), respectively) and Ca2+-activated K+ (I(KCa)) currents in cultured bovine chromaffin cells were investigated using the whole-cell patch-clamp technique. Superfusion with BAPTA-AM (50 μM) induced a rapid (< 60 s) and reversible block of both I(KCa) and I(K) (~50%), without affecting either I(Ca) or I(Na). Preincubation with BAPTA-AM (50 μM, 30 min) or cell loading with the non- permeable active form of BAPTA (10 mM in the pipette solution) permanently blocked I(KCa). BAPTA-AM superfusion (50 μM) also blocked I(K) (~53%) after BAPTA-loading or BAPTA-AM preincubation. In conclusion, we show a fast and reversible block of I(KCa) and I(K) by BAPTA-AM, acting directly on K+ channels before it operates as a Ca2+ chelator, in cultured bovine chromaffin cells.
title BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells
title_short BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells
title_full BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells
title_fullStr BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells
title_full_unstemmed BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells
title_sort bapta-am blocks both voltage-gated and ca2+-activated k+ currents in cultured bovine chromaffin cells
publishDate 1998
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v9_n15_p3403_Urbano
http://hdl.handle.net/20.500.12110/paper_09594965_v9_n15_p3403_Urbano
_version_ 1768544283913093120

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