Inhibition of DNA synthesis in human gliomas by roscovitine
The early effect of 1-100 μM roscovitine, a purine analogue and cyclin- dependent kinase inhibitor, was studied on tissue specimens from eight human malignant gliomas. The tissue was incubated immediately after resection with DMEM containing [3H]methyl-thymidine plus vehicle alone or the proper conc...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v10_n12_p2563_Yakisich http://hdl.handle.net/20.500.12110/paper_09594965_v10_n12_p2563_Yakisich |
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paper:paper_09594965_v10_n12_p2563_Yakisich |
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paper:paper_09594965_v10_n12_p2563_Yakisich2023-06-08T15:56:58Z Inhibition of DNA synthesis in human gliomas by roscovitine Brain tumors Cyclin-dependent kinases DNA synthesis Gliomas Proliferation rate Roscovitine lomustine roscovitine adult article concentration response DNA synthesis inhibition drug effect female glioblastoma glioma human human tissue male priority journal The early effect of 1-100 μM roscovitine, a purine analogue and cyclin- dependent kinase inhibitor, was studied on tissue specimens from eight human malignant gliomas. The tissue was incubated immediately after resection with DMEM containing [3H]methyl-thymidine plus vehicle alone or the proper concentration of roscovitine for 33-90 min. The DNA synthesis rate was assessed by measurement of [3H]methyl-thymidine incorporation into trichloroacetic acid insoluble material/mg protein/min. In all gliomas, 100 μM roscovitine inhibited DNA synthesis by 71-97% (average 89 ± 8%, p < 0.0001). This inhibitory effect of roscovitine appeared within 30 min of incubation and was concentration dependent. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v10_n12_p2563_Yakisich http://hdl.handle.net/20.500.12110/paper_09594965_v10_n12_p2563_Yakisich |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Brain tumors Cyclin-dependent kinases DNA synthesis Gliomas Proliferation rate Roscovitine lomustine roscovitine adult article concentration response DNA synthesis inhibition drug effect female glioblastoma glioma human human tissue male priority journal |
| spellingShingle |
Brain tumors Cyclin-dependent kinases DNA synthesis Gliomas Proliferation rate Roscovitine lomustine roscovitine adult article concentration response DNA synthesis inhibition drug effect female glioblastoma glioma human human tissue male priority journal Inhibition of DNA synthesis in human gliomas by roscovitine |
| topic_facet |
Brain tumors Cyclin-dependent kinases DNA synthesis Gliomas Proliferation rate Roscovitine lomustine roscovitine adult article concentration response DNA synthesis inhibition drug effect female glioblastoma glioma human human tissue male priority journal |
| description |
The early effect of 1-100 μM roscovitine, a purine analogue and cyclin- dependent kinase inhibitor, was studied on tissue specimens from eight human malignant gliomas. The tissue was incubated immediately after resection with DMEM containing [3H]methyl-thymidine plus vehicle alone or the proper concentration of roscovitine for 33-90 min. The DNA synthesis rate was assessed by measurement of [3H]methyl-thymidine incorporation into trichloroacetic acid insoluble material/mg protein/min. In all gliomas, 100 μM roscovitine inhibited DNA synthesis by 71-97% (average 89 ± 8%, p < 0.0001). This inhibitory effect of roscovitine appeared within 30 min of incubation and was concentration dependent. |
| title |
Inhibition of DNA synthesis in human gliomas by roscovitine |
| title_short |
Inhibition of DNA synthesis in human gliomas by roscovitine |
| title_full |
Inhibition of DNA synthesis in human gliomas by roscovitine |
| title_fullStr |
Inhibition of DNA synthesis in human gliomas by roscovitine |
| title_full_unstemmed |
Inhibition of DNA synthesis in human gliomas by roscovitine |
| title_sort |
inhibition of dna synthesis in human gliomas by roscovitine |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v10_n12_p2563_Yakisich http://hdl.handle.net/20.500.12110/paper_09594965_v10_n12_p2563_Yakisich |
| _version_ |
1768543620057530368 |