Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation

Six Rhizobium meliloti mutants were isolated after Tn5-mediated mutagenesis as resistant to inhibition by a mixture of amino acids (serine, methionine, glycine and leucine). All were defective in adenylate cyclase activity and failed to form nodules in infected roots of Medicago sativa. Furthermore,...

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Autores principales:	Bianchini, Graciela Mabel, Carricarte, Valentina, Flawiá, Mirtha María
Publicado:	1993
Materias:	Adenylate cyclase amino acid sensitivity exopolysaccharide nodulation Rhizobium meliloti Tn5 mutagenesis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v9_n2_p168_Bianchini http://hdl.handle.net/20.500.12110/paper_09593993_v9_n2_p168_Bianchini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09593993_v9_n2_p168_Bianchini
record_format	dspace
spelling	paper:paper_09593993_v9_n2_p168_Bianchini2023-06-08T15:56:55Z Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation Bianchini, Graciela Mabel Carricarte, Valentina Flawiá, Mirtha María Adenylate cyclase amino acid sensitivity exopolysaccharide nodulation Rhizobium meliloti Tn5 mutagenesis Six Rhizobium meliloti mutants were isolated after Tn5-mediated mutagenesis as resistant to inhibition by a mixture of amino acids (serine, methionine, glycine and leucine). All were defective in adenylate cyclase activity and failed to form nodules in infected roots of Medicago sativa. Furthermore, like other nodulation mutants, they showed altered motility and increased secretion of exopolysaccharides; addition of cAMP to the growth medium abolished some of these phenotypic defects. The possibility that adenylate cyclase participates in the transduction of signals inducing nodulation is discussed. © 1993 Rapid Communications of Oxford Ltd. Fil:Bianchini, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carricarte, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v9_n2_p168_Bianchini http://hdl.handle.net/20.500.12110/paper_09593993_v9_n2_p168_Bianchini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adenylate cyclase amino acid sensitivity exopolysaccharide nodulation Rhizobium meliloti Tn5 mutagenesis
spellingShingle	Adenylate cyclase amino acid sensitivity exopolysaccharide nodulation Rhizobium meliloti Tn5 mutagenesis Bianchini, Graciela Mabel Carricarte, Valentina Flawiá, Mirtha María Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
topic_facet	Adenylate cyclase amino acid sensitivity exopolysaccharide nodulation Rhizobium meliloti Tn5 mutagenesis
description	Six Rhizobium meliloti mutants were isolated after Tn5-mediated mutagenesis as resistant to inhibition by a mixture of amino acids (serine, methionine, glycine and leucine). All were defective in adenylate cyclase activity and failed to form nodules in infected roots of Medicago sativa. Furthermore, like other nodulation mutants, they showed altered motility and increased secretion of exopolysaccharides; addition of cAMP to the growth medium abolished some of these phenotypic defects. The possibility that adenylate cyclase participates in the transduction of signals inducing nodulation is discussed. © 1993 Rapid Communications of Oxford Ltd.
author	Bianchini, Graciela Mabel Carricarte, Valentina Flawiá, Mirtha María
author_facet	Bianchini, Graciela Mabel Carricarte, Valentina Flawiá, Mirtha María
author_sort	Bianchini, Graciela Mabel
title	Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_short	Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_full	Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_fullStr	Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_full_unstemmed	Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
title_sort	isolation of adenylate cyclase mutants from rhizobium meliloti deficient in nodulation
publishDate	1993
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v9_n2_p168_Bianchini http://hdl.handle.net/20.500.12110/paper_09593993_v9_n2_p168_Bianchini
work_keys_str_mv	AT bianchinigracielamabel isolationofadenylatecyclasemutantsfromrhizobiummelilotideficientinnodulation AT carricartevalentina isolationofadenylatecyclasemutantsfromrhizobiummelilotideficientinnodulation AT flawiamirthamaria isolationofadenylatecyclasemutantsfromrhizobiummelilotideficientinnodulation
_version_	1768545152424476672

Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation

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