Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds

In this work a new Bacillus sp. strain, isolated from honey, was characterized phylogenetically. Its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mas...

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Guardado en:
Detalles Bibliográficos
Publicado: 2015
Materias:
Bacillus subtilis subsp. subtilis
Foodborne pathogens
Lipopeptide homologues
Surfactin
UV-MALDI-TOF MS
Bacteria
Bacteriology
Cell culture
Diseases
Inductively coupled plasma
Listeria
Mass spectrometry
Metabolites
Pathogens
Polypeptides
RNA
Spectrometry
Strain
Food-borne pathogens
Lipopeptides
MALDI TOF MS
Subtilis
Food microbiology
antiinfective agent
bacterial DNA
biological product
DNA topoisomerase (ATP hydrolysing)
ribosome DNA
RNA 16S
antibiosis
Bacillus subtilis
chemistry
classification
cluster analysis
DNA sequence
food control
genetics
Gram positive bacterium
growth, development and aging
isolation and purification
mass spectrometry
metabolism
molecular genetics
phylogeny
physiology
ultraviolet spectrophotometry
Anti-Bacterial Agents
Antibiosis
Biological Products
Cluster Analysis
DNA Gyrase
DNA, Bacterial
DNA, Ribosomal
Food Microbiology
Gram-Positive Bacteria
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrophotometry, Ultraviolet
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v31_n6_p929_Torres
http://hdl.handle.net/20.500.12110/paper_09593993_v31_n6_p929_Torres
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:In this work a new Bacillus sp. strain, isolated from honey, was characterized phylogenetically. Its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Bacillus CBMDC3f was phylogenetically characterized as Bacillus subtilis subsp. subtilis after rRNA analysis of the 16S subunit and the gyrA gene (access codes Genbank JX120508 and JX120516, respectively). Its antibacterial potential was evaluated against Listeria monocytogenes (9 strains), B. cereus (3 strains) and Staphylococcus aureus ATCC29213. Its cell suspension and cell-free supernatant (CFS) exerted significant anti-Listeria and anti-S. aureus activities, while the lipopeptides fraction (LF) also showed anti-B. cereus effect. The UV-MALDI-MS analysis revealed surfactin, iturin and fengycin in the CFS, whereas surfactin predominated in the LF. The CFS from CBMDC3f contained surfactin, iturin and fengycin with four, two and four homologues per family, respectively, whereas four surfactin, one iturin and one fengycin homologues were identified in the LF. For some surfactin homologues, their UV-MALDI-TOF/TOF (MS/MS; Laser Induced Decomposition method, LID) spectra were also obtained. Mass spectrometry analysis contributed with relevant information about the type of lipopeptides that Bacillus strains can synthesize. From our results, surfactin would be the main metabolite responsible for the antibacterial effect. © 2015, Springer Science+Business Media Dordrecht.

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