Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds

In this work a new Bacillus sp. strain, isolated from honey, was characterized phylogenetically. Its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mas...

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Publicado:	2015
Materias:	Bacillus subtilis subsp. subtilis Foodborne pathogens Lipopeptide homologues Surfactin UV-MALDI-TOF MS Bacteria Bacteriology Cell culture Diseases Inductively coupled plasma Listeria Mass spectrometry Metabolites Pathogens Polypeptides RNA Spectrometry Strain Food-borne pathogens Lipopeptides MALDI TOF MS Subtilis Food microbiology antiinfective agent bacterial DNA biological product DNA topoisomerase (ATP hydrolysing) ribosome DNA RNA 16S antibiosis Bacillus subtilis chemistry classification cluster analysis DNA sequence food control genetics Gram positive bacterium growth, development and aging isolation and purification mass spectrometry metabolism molecular genetics phylogeny physiology ultraviolet spectrophotometry Anti-Bacterial Agents Antibiosis Biological Products Cluster Analysis DNA Gyrase DNA, Bacterial DNA, Ribosomal Food Microbiology Gram-Positive Bacteria Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v31_n6_p929_Torres http://hdl.handle.net/20.500.12110/paper_09593993_v31_n6_p929_Torres
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09593993_v31_n6_p929_Torres
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spelling	paper:paper_09593993_v31_n6_p929_Torres2023-06-08T15:56:54Z Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds Bacillus subtilis subsp. subtilis Foodborne pathogens Lipopeptide homologues Surfactin UV-MALDI-TOF MS Bacteria Bacteriology Cell culture Diseases Inductively coupled plasma Listeria Mass spectrometry Metabolites Pathogens Polypeptides RNA Spectrometry Strain Food-borne pathogens Lipopeptides MALDI TOF MS Subtilis Surfactin Food microbiology antiinfective agent bacterial DNA biological product DNA topoisomerase (ATP hydrolysing) ribosome DNA RNA 16S antibiosis Bacillus subtilis chemistry classification cluster analysis DNA sequence food control genetics Gram positive bacterium growth, development and aging isolation and purification mass spectrometry metabolism molecular genetics phylogeny physiology ultraviolet spectrophotometry Anti-Bacterial Agents Antibiosis Bacillus subtilis Biological Products Cluster Analysis DNA Gyrase DNA, Bacterial DNA, Ribosomal Food Microbiology Gram-Positive Bacteria Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet In this work a new Bacillus sp. strain, isolated from honey, was characterized phylogenetically. Its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Bacillus CBMDC3f was phylogenetically characterized as Bacillus subtilis subsp. subtilis after rRNA analysis of the 16S subunit and the gyrA gene (access codes Genbank JX120508 and JX120516, respectively). Its antibacterial potential was evaluated against Listeria monocytogenes (9 strains), B. cereus (3 strains) and Staphylococcus aureus ATCC29213. Its cell suspension and cell-free supernatant (CFS) exerted significant anti-Listeria and anti-S. aureus activities, while the lipopeptides fraction (LF) also showed anti-B. cereus effect. The UV-MALDI-MS analysis revealed surfactin, iturin and fengycin in the CFS, whereas surfactin predominated in the LF. The CFS from CBMDC3f contained surfactin, iturin and fengycin with four, two and four homologues per family, respectively, whereas four surfactin, one iturin and one fengycin homologues were identified in the LF. For some surfactin homologues, their UV-MALDI-TOF/TOF (MS/MS; Laser Induced Decomposition method, LID) spectra were also obtained. Mass spectrometry analysis contributed with relevant information about the type of lipopeptides that Bacillus strains can synthesize. From our results, surfactin would be the main metabolite responsible for the antibacterial effect. © 2015, Springer Science+Business Media Dordrecht. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v31_n6_p929_Torres http://hdl.handle.net/20.500.12110/paper_09593993_v31_n6_p929_Torres
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacillus subtilis subsp. subtilis Foodborne pathogens Lipopeptide homologues Surfactin UV-MALDI-TOF MS Bacteria Bacteriology Cell culture Diseases Inductively coupled plasma Listeria Mass spectrometry Metabolites Pathogens Polypeptides RNA Spectrometry Strain Food-borne pathogens Lipopeptides MALDI TOF MS Subtilis Surfactin Food microbiology antiinfective agent bacterial DNA biological product DNA topoisomerase (ATP hydrolysing) ribosome DNA RNA 16S antibiosis Bacillus subtilis chemistry classification cluster analysis DNA sequence food control genetics Gram positive bacterium growth, development and aging isolation and purification mass spectrometry metabolism molecular genetics phylogeny physiology ultraviolet spectrophotometry Anti-Bacterial Agents Antibiosis Bacillus subtilis Biological Products Cluster Analysis DNA Gyrase DNA, Bacterial DNA, Ribosomal Food Microbiology Gram-Positive Bacteria Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet
spellingShingle	Bacillus subtilis subsp. subtilis Foodborne pathogens Lipopeptide homologues Surfactin UV-MALDI-TOF MS Bacteria Bacteriology Cell culture Diseases Inductively coupled plasma Listeria Mass spectrometry Metabolites Pathogens Polypeptides RNA Spectrometry Strain Food-borne pathogens Lipopeptides MALDI TOF MS Subtilis Surfactin Food microbiology antiinfective agent bacterial DNA biological product DNA topoisomerase (ATP hydrolysing) ribosome DNA RNA 16S antibiosis Bacillus subtilis chemistry classification cluster analysis DNA sequence food control genetics Gram positive bacterium growth, development and aging isolation and purification mass spectrometry metabolism molecular genetics phylogeny physiology ultraviolet spectrophotometry Anti-Bacterial Agents Antibiosis Bacillus subtilis Biological Products Cluster Analysis DNA Gyrase DNA, Bacterial DNA, Ribosomal Food Microbiology Gram-Positive Bacteria Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds
topic_facet	Bacillus subtilis subsp. subtilis Foodborne pathogens Lipopeptide homologues Surfactin UV-MALDI-TOF MS Bacteria Bacteriology Cell culture Diseases Inductively coupled plasma Listeria Mass spectrometry Metabolites Pathogens Polypeptides RNA Spectrometry Strain Food-borne pathogens Lipopeptides MALDI TOF MS Subtilis Surfactin Food microbiology antiinfective agent bacterial DNA biological product DNA topoisomerase (ATP hydrolysing) ribosome DNA RNA 16S antibiosis Bacillus subtilis chemistry classification cluster analysis DNA sequence food control genetics Gram positive bacterium growth, development and aging isolation and purification mass spectrometry metabolism molecular genetics phylogeny physiology ultraviolet spectrophotometry Anti-Bacterial Agents Antibiosis Bacillus subtilis Biological Products Cluster Analysis DNA Gyrase DNA, Bacterial DNA, Ribosomal Food Microbiology Gram-Positive Bacteria Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet
description	In this work a new Bacillus sp. strain, isolated from honey, was characterized phylogenetically. Its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Bacillus CBMDC3f was phylogenetically characterized as Bacillus subtilis subsp. subtilis after rRNA analysis of the 16S subunit and the gyrA gene (access codes Genbank JX120508 and JX120516, respectively). Its antibacterial potential was evaluated against Listeria monocytogenes (9 strains), B. cereus (3 strains) and Staphylococcus aureus ATCC29213. Its cell suspension and cell-free supernatant (CFS) exerted significant anti-Listeria and anti-S. aureus activities, while the lipopeptides fraction (LF) also showed anti-B. cereus effect. The UV-MALDI-MS analysis revealed surfactin, iturin and fengycin in the CFS, whereas surfactin predominated in the LF. The CFS from CBMDC3f contained surfactin, iturin and fengycin with four, two and four homologues per family, respectively, whereas four surfactin, one iturin and one fengycin homologues were identified in the LF. For some surfactin homologues, their UV-MALDI-TOF/TOF (MS/MS; Laser Induced Decomposition method, LID) spectra were also obtained. Mass spectrometry analysis contributed with relevant information about the type of lipopeptides that Bacillus strains can synthesize. From our results, surfactin would be the main metabolite responsible for the antibacterial effect. © 2015, Springer Science+Business Media Dordrecht.
title	Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds
title_short	Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds
title_full	Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds
title_fullStr	Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds
title_full_unstemmed	Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds
title_sort	bacillus subtilis subsp. subtilis cbmdc3f with antimicrobial activity against gram-positive foodborne pathogenic bacteria: uv-maldi-tof ms analysis of its bioactive compounds
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v31_n6_p929_Torres http://hdl.handle.net/20.500.12110/paper_09593993_v31_n6_p929_Torres
_version_	1768544785795121152

Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds

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