Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake)
Cultivation of specialty mushrooms on lignocellulosic wastes represents one of the most economical organic recycling processes. Compared with other cultivated mushrooms, very little is known about the nature of the lignocellulolytic enzymes produced by the edible and medicinal fungus Grifola frondos...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n4_p1533_Montoya http://hdl.handle.net/20.500.12110/paper_09593993_v28_n4_p1533_Montoya |
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paper:paper_09593993_v28_n4_p1533_Montoya2023-06-08T15:56:54Z Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake) Levin, Laura Noemi Coffee spent-ground Grifola frondosa Lignocellulolytic enzymes Solid state fermentation White-rot fungi Activity profile Biological efficiency Cellulase activity Corn bran Dry substrates Edible mushroom Endoglucanases Endoxylanase Exoglucanase Fruiting bodies Glucosidase Grifola frondosa Laccases Lignin contents Lignocellulolytic enzymes Lignocellulosic wastes Medicinal fungus Mushroom production Oak sawdust Organic recycling Polysaccharide production Qualitative assays Solid-state fermentation White rot fungi Xylanases Byproducts Cellulose Enzyme activity Fermentation Fungi Lignin Manganese Substrates cellulase lignin lignin peroxidase lignocellulose peroxidase article chemistry culture medium enzymology Grifola growth, development and aging industrial waste metabolism Cellulases Culture Media Grifola Industrial Waste Lignin Peroxidases Agaricus bisporus Basidiomycota Fungi Grifola frondosa Zea mays Cultivation of specialty mushrooms on lignocellulosic wastes represents one of the most economical organic recycling processes. Compared with other cultivated mushrooms, very little is known about the nature of the lignocellulolytic enzymes produced by the edible and medicinal fungus Grifola frondosa, the parameters affecting their production, and enzyme activity profiles during different stages of the developmental cycle. In this work we investigated the enzymes that enable G. frondosa, to colonize and deconstruct two formulations based on industrial lignocellulosic by-products. G. frondosa degraded both substrates (oak-sawdust plus corn bran, and oak/corn bran supplemented with coffee spent-ground) decreasing 67 and 50% of their lignin content, along with 44 and 37% of the polysaccharides (hemicellulose and cellulose) respectively. 35.3% biological efficiency was obtained when using oak sawdust plus corn bran as substrate. Coffee spent-ground addition inhibited mushroom production, decreased growth, xylanase and cellulase activities. However, taking into account that G. frondosa successfully colonized this residue; this substrate formula might be considered for its growth and medicinal polysaccharide production. Although G. frondosa tested positive for Azure B plate degradation, a qualitative assay for lignin-peroxidase, attempts to detect this activity during solid state fermentation were unsuccessful. Enzyme activities peaked during colonization but declined drastically during fruiting body formation. Highest activities achieved were: endoglucanase 12.3, exoglucanase 16.2, β-glucosidase 2.3, endoxylanase 20.3, amylase 0.26, laccase 14.8 and Mn-peroxidase 7.4 U/g dry substrate. © 2011 Springer Science+Business Media B.V. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n4_p1533_Montoya http://hdl.handle.net/20.500.12110/paper_09593993_v28_n4_p1533_Montoya |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Coffee spent-ground Grifola frondosa Lignocellulolytic enzymes Solid state fermentation White-rot fungi Activity profile Biological efficiency Cellulase activity Corn bran Dry substrates Edible mushroom Endoglucanases Endoxylanase Exoglucanase Fruiting bodies Glucosidase Grifola frondosa Laccases Lignin contents Lignocellulolytic enzymes Lignocellulosic wastes Medicinal fungus Mushroom production Oak sawdust Organic recycling Polysaccharide production Qualitative assays Solid-state fermentation White rot fungi Xylanases Byproducts Cellulose Enzyme activity Fermentation Fungi Lignin Manganese Substrates cellulase lignin lignin peroxidase lignocellulose peroxidase article chemistry culture medium enzymology Grifola growth, development and aging industrial waste metabolism Cellulases Culture Media Grifola Industrial Waste Lignin Peroxidases Agaricus bisporus Basidiomycota Fungi Grifola frondosa Zea mays |
| spellingShingle |
Coffee spent-ground Grifola frondosa Lignocellulolytic enzymes Solid state fermentation White-rot fungi Activity profile Biological efficiency Cellulase activity Corn bran Dry substrates Edible mushroom Endoglucanases Endoxylanase Exoglucanase Fruiting bodies Glucosidase Grifola frondosa Laccases Lignin contents Lignocellulolytic enzymes Lignocellulosic wastes Medicinal fungus Mushroom production Oak sawdust Organic recycling Polysaccharide production Qualitative assays Solid-state fermentation White rot fungi Xylanases Byproducts Cellulose Enzyme activity Fermentation Fungi Lignin Manganese Substrates cellulase lignin lignin peroxidase lignocellulose peroxidase article chemistry culture medium enzymology Grifola growth, development and aging industrial waste metabolism Cellulases Culture Media Grifola Industrial Waste Lignin Peroxidases Agaricus bisporus Basidiomycota Fungi Grifola frondosa Zea mays Levin, Laura Noemi Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake) |
| topic_facet |
Coffee spent-ground Grifola frondosa Lignocellulolytic enzymes Solid state fermentation White-rot fungi Activity profile Biological efficiency Cellulase activity Corn bran Dry substrates Edible mushroom Endoglucanases Endoxylanase Exoglucanase Fruiting bodies Glucosidase Grifola frondosa Laccases Lignin contents Lignocellulolytic enzymes Lignocellulosic wastes Medicinal fungus Mushroom production Oak sawdust Organic recycling Polysaccharide production Qualitative assays Solid-state fermentation White rot fungi Xylanases Byproducts Cellulose Enzyme activity Fermentation Fungi Lignin Manganese Substrates cellulase lignin lignin peroxidase lignocellulose peroxidase article chemistry culture medium enzymology Grifola growth, development and aging industrial waste metabolism Cellulases Culture Media Grifola Industrial Waste Lignin Peroxidases Agaricus bisporus Basidiomycota Fungi Grifola frondosa Zea mays |
| description |
Cultivation of specialty mushrooms on lignocellulosic wastes represents one of the most economical organic recycling processes. Compared with other cultivated mushrooms, very little is known about the nature of the lignocellulolytic enzymes produced by the edible and medicinal fungus Grifola frondosa, the parameters affecting their production, and enzyme activity profiles during different stages of the developmental cycle. In this work we investigated the enzymes that enable G. frondosa, to colonize and deconstruct two formulations based on industrial lignocellulosic by-products. G. frondosa degraded both substrates (oak-sawdust plus corn bran, and oak/corn bran supplemented with coffee spent-ground) decreasing 67 and 50% of their lignin content, along with 44 and 37% of the polysaccharides (hemicellulose and cellulose) respectively. 35.3% biological efficiency was obtained when using oak sawdust plus corn bran as substrate. Coffee spent-ground addition inhibited mushroom production, decreased growth, xylanase and cellulase activities. However, taking into account that G. frondosa successfully colonized this residue; this substrate formula might be considered for its growth and medicinal polysaccharide production. Although G. frondosa tested positive for Azure B plate degradation, a qualitative assay for lignin-peroxidase, attempts to detect this activity during solid state fermentation were unsuccessful. Enzyme activities peaked during colonization but declined drastically during fruiting body formation. Highest activities achieved were: endoglucanase 12.3, exoglucanase 16.2, β-glucosidase 2.3, endoxylanase 20.3, amylase 0.26, laccase 14.8 and Mn-peroxidase 7.4 U/g dry substrate. © 2011 Springer Science+Business Media B.V. |
| author |
Levin, Laura Noemi |
| author_facet |
Levin, Laura Noemi |
| author_sort |
Levin, Laura Noemi |
| title |
Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake) |
| title_short |
Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake) |
| title_full |
Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake) |
| title_fullStr |
Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake) |
| title_full_unstemmed |
Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake) |
| title_sort |
growth, fruiting and lignocellulolytic enzyme production by the edible mushroom grifola frondosa (maitake) |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n4_p1533_Montoya http://hdl.handle.net/20.500.12110/paper_09593993_v28_n4_p1533_Montoya |
| work_keys_str_mv |
AT levinlauranoemi growthfruitingandlignocellulolyticenzymeproductionbytheediblemushroomgrifolafrondosamaitake |
| _version_ |
1768546548552040448 |