Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v25_n10_p1857_Fernandez http://hdl.handle.net/20.500.12110/paper_09593993_v25_n10_p1857_Fernandez |
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paper:paper_09593993_v25_n10_p1857_Fernandez2023-06-08T15:56:53Z Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis Fernández, Laura Silvani, Vanesa Analía Bompadre, María Josefina Pérgola, Mariana Godeas, Alicia Margarita Glomus intraradices Monoxenic culture Transformed soybean roots Absorbing structure Am fungus Arbuscular mycorrhizal fungi Arbuscular mycorrhizal symbiosis Characteristic development Cortical cells Glomus intraradices Glycine max In-plants In-vitro Intensity values Plant hosts Soybean roots Whole plants Amplitude modulation Nitrogen fixation Nucleic acids Plants (botany) Cell culture Arbuscular Bacteria (microorganisms) Fungi Glomus intraradices Glycine max Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made between TSR system and plants of the same genotype. The extraradical fungal structures generated in vitro culture showed normal development. Straight runner hyphae branched into short simple branched absorbing structures and spores were initiated. AM symbiosis was confirmed by the presence of arbuscules and vesicles in cortical cells of the TSRs. The frequency of intraradical colonization in TSRs was higher than in plants grown in soil, whereas the intensity values of intraradical colonization in TSR cultures were similar to those in whole plants. These results show that TSR cultures were able to support the growth and characteristic development of G. intraradices. © 2009 Springer Science+Business Media B.V. Fil:Fernández, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silvani, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bompadre, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérgola, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v25_n10_p1857_Fernandez http://hdl.handle.net/20.500.12110/paper_09593993_v25_n10_p1857_Fernandez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Glomus intraradices Monoxenic culture Transformed soybean roots Absorbing structure Am fungus Arbuscular mycorrhizal fungi Arbuscular mycorrhizal symbiosis Characteristic development Cortical cells Glomus intraradices Glycine max In-plants In-vitro Intensity values Plant hosts Soybean roots Whole plants Amplitude modulation Nitrogen fixation Nucleic acids Plants (botany) Cell culture Arbuscular Bacteria (microorganisms) Fungi Glomus intraradices Glycine max |
| spellingShingle |
Glomus intraradices Monoxenic culture Transformed soybean roots Absorbing structure Am fungus Arbuscular mycorrhizal fungi Arbuscular mycorrhizal symbiosis Characteristic development Cortical cells Glomus intraradices Glycine max In-plants In-vitro Intensity values Plant hosts Soybean roots Whole plants Amplitude modulation Nitrogen fixation Nucleic acids Plants (botany) Cell culture Arbuscular Bacteria (microorganisms) Fungi Glomus intraradices Glycine max Fernández, Laura Silvani, Vanesa Analía Bompadre, María Josefina Pérgola, Mariana Godeas, Alicia Margarita Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis |
| topic_facet |
Glomus intraradices Monoxenic culture Transformed soybean roots Absorbing structure Am fungus Arbuscular mycorrhizal fungi Arbuscular mycorrhizal symbiosis Characteristic development Cortical cells Glomus intraradices Glycine max In-plants In-vitro Intensity values Plant hosts Soybean roots Whole plants Amplitude modulation Nitrogen fixation Nucleic acids Plants (botany) Cell culture Arbuscular Bacteria (microorganisms) Fungi Glomus intraradices Glycine max |
| description |
Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made between TSR system and plants of the same genotype. The extraradical fungal structures generated in vitro culture showed normal development. Straight runner hyphae branched into short simple branched absorbing structures and spores were initiated. AM symbiosis was confirmed by the presence of arbuscules and vesicles in cortical cells of the TSRs. The frequency of intraradical colonization in TSRs was higher than in plants grown in soil, whereas the intensity values of intraradical colonization in TSR cultures were similar to those in whole plants. These results show that TSR cultures were able to support the growth and characteristic development of G. intraradices. © 2009 Springer Science+Business Media B.V. |
| author |
Fernández, Laura Silvani, Vanesa Analía Bompadre, María Josefina Pérgola, Mariana Godeas, Alicia Margarita |
| author_facet |
Fernández, Laura Silvani, Vanesa Analía Bompadre, María Josefina Pérgola, Mariana Godeas, Alicia Margarita |
| author_sort |
Fernández, Laura |
| title |
Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis |
| title_short |
Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis |
| title_full |
Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis |
| title_fullStr |
Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis |
| title_full_unstemmed |
Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis |
| title_sort |
transformed soybean (glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v25_n10_p1857_Fernandez http://hdl.handle.net/20.500.12110/paper_09593993_v25_n10_p1857_Fernandez |
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