Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid

An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarate from 2-oxoglutaric acid. An innovative combination strategy that uses pure...

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Autores principales:	Rustoy, Eduardo Miguel, Pereyra, Elba Nora, Moreno de Colonna, Silvia, Baldessari, Alicia
Publicado:	2004
Materias:	2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09574166_v15_n23_p3763_Rustoy http://hdl.handle.net/20.500.12110/paper_09574166_v15_n23_p3763_Rustoy
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09574166_v15_n23_p3763_Rustoy
record_format	dspace
spelling	paper:paper_09574166_v15_n23_p3763_Rustoy2025-07-30T18:32:38Z Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid Rustoy, Eduardo Miguel Pereyra, Elba Nora Moreno de Colonna, Silvia Baldessari, Alicia 2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarate from 2-oxoglutaric acid. An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarates from 2-oxoglutaric acid. The method involves two consecutive biocatalytic steps. The first step, which converts the 2-oxoglutaric acid into the corresponding dialkyl esters, was catalyzed by a lipase. Then in the second step, by microbial reduction of the dialkyl-2-oxoglutarates, it is possible to obtain 3-carboxyalkyl-γ- butyrolactones or 2-hydroxyesters depending on the length of the chain in the alkyl moiety of the esters and on the fresh or lyophilized status of the cells. © 2004 Elsevier Ltd. All rights reserved. Fil:Rustoy, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereyra, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Baldessari, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09574166_v15_n23_p3763_Rustoy http://hdl.handle.net/20.500.12110/paper_09574166_v15_n23_p3763_Rustoy
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal
spellingShingle	2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal Rustoy, Eduardo Miguel Pereyra, Elba Nora Moreno de Colonna, Silvia Baldessari, Alicia Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid
topic_facet	2 oxoglutaric acid ester derivative fungal enzyme lactone derivative triacylglycerol lipase acidification alkylation article biocatalyst catalysis derivatization drug synthesis enantiomer enzyme mechanism esterification lactonization priority journal
description	An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarate from 2-oxoglutaric acid. An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarates from 2-oxoglutaric acid. The method involves two consecutive biocatalytic steps. The first step, which converts the 2-oxoglutaric acid into the corresponding dialkyl esters, was catalyzed by a lipase. Then in the second step, by microbial reduction of the dialkyl-2-oxoglutarates, it is possible to obtain 3-carboxyalkyl-γ- butyrolactones or 2-hydroxyesters depending on the length of the chain in the alkyl moiety of the esters and on the fresh or lyophilized status of the cells. © 2004 Elsevier Ltd. All rights reserved.
author	Rustoy, Eduardo Miguel Pereyra, Elba Nora Moreno de Colonna, Silvia Baldessari, Alicia
author_facet	Rustoy, Eduardo Miguel Pereyra, Elba Nora Moreno de Colonna, Silvia Baldessari, Alicia
author_sort	Rustoy, Eduardo Miguel
title	Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid
title_short	Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid
title_full	Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid
title_fullStr	Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid
title_full_unstemmed	Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid
title_sort	combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09574166_v15_n23_p3763_Rustoy http://hdl.handle.net/20.500.12110/paper_09574166_v15_n23_p3763_Rustoy
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_version_	1840321979409760256

Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid

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