Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications
We present the construction of layer-by-layer (LbL) assemblies of polyethylenimine and urease onto reduced-graphene-oxide based field-effect transistors (rGO FETs) for the detection of urea. This versatile biosensor platform simultaneously exploits the pH dependency of liquid-gated graphene-based tr...
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paper:paper_09565663_v92_n_p661_Piccinini2023-06-08T15:56:15Z Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications Battaglini, Fernando Biosensors Chemical sensors Field-effect transistors Graphene Biosensors Chemical sensors Copper Enzymes Graphene Graphene transistors Heavy metals Metabolism pH sensors Polyelectrolytes Solutions Transistors Urea Bio-sensor platforms Biosensing applications Hydrolysis of ureas Layer-by-layer assemblies Long term stability Polyelectrolyte multilayer Reduced graphene oxides Weak polyelectrolytes Field effect transistors copper ion graphene oxide polyelectrolyte polyethyleneimine urease graphite immobilized enzyme oxide polyelectrolyte urea urease aqueous solution Article biosensor catalysis enzyme analysis field effect transistor hydrolysis limit of detection pH measurement surface property blood Canavalia chemistry devices enzymology equipment design evaluation study genetic procedures human transistor Biosensing Techniques Canavalia Enzymes, Immobilized Equipment Design Graphite Humans Limit of Detection Oxides Polyelectrolytes Transistors, Electronic Urea Urease We present the construction of layer-by-layer (LbL) assemblies of polyethylenimine and urease onto reduced-graphene-oxide based field-effect transistors (rGO FETs) for the detection of urea. This versatile biosensor platform simultaneously exploits the pH dependency of liquid-gated graphene-based transistors and the change in the local pH produced by the catalyzed hydrolysis of urea. The use of an interdigitated microchannel resulted in transistors displaying low noise, high pH sensitivity (20.3 µA/pH) and transconductance values up to 800 µS. The modification of rGO FETs with a weak polyelectrolyte improved the pH response because of its transducing properties by electrostatic gating effects. In the presence of urea, the urease-modified rGO FETs showed a shift in the Dirac point due to the change in the local pH close to the graphene surface. Markedly, these devices operated at very low voltages (less than 500 mV) and were able to monitor urea in the range of 1–1000 µm, with a limit of detection (LOD) down to 1 µm, fast response and good long-term stability. The urea-response of the transistors was enhanced by increasing the number of bilayers due to the increment of the enzyme surface coverage onto the channel. Moreover, quantification of the heavy metal Cu 2+ (with a LOD down to 10 nM) was performed in aqueous solution by taking advantage of the urease specific inhibition. © 2016 The Authors Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09565663_v92_n_p661_Piccinini http://hdl.handle.net/20.500.12110/paper_09565663_v92_n_p661_Piccinini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biosensors Chemical sensors Field-effect transistors Graphene Biosensors Chemical sensors Copper Enzymes Graphene Graphene transistors Heavy metals Metabolism pH sensors Polyelectrolytes Solutions Transistors Urea Bio-sensor platforms Biosensing applications Hydrolysis of ureas Layer-by-layer assemblies Long term stability Polyelectrolyte multilayer Reduced graphene oxides Weak polyelectrolytes Field effect transistors copper ion graphene oxide polyelectrolyte polyethyleneimine urease graphite immobilized enzyme oxide polyelectrolyte urea urease aqueous solution Article biosensor catalysis enzyme analysis field effect transistor hydrolysis limit of detection pH measurement surface property blood Canavalia chemistry devices enzymology equipment design evaluation study genetic procedures human transistor Biosensing Techniques Canavalia Enzymes, Immobilized Equipment Design Graphite Humans Limit of Detection Oxides Polyelectrolytes Transistors, Electronic Urea Urease |
| spellingShingle |
Biosensors Chemical sensors Field-effect transistors Graphene Biosensors Chemical sensors Copper Enzymes Graphene Graphene transistors Heavy metals Metabolism pH sensors Polyelectrolytes Solutions Transistors Urea Bio-sensor platforms Biosensing applications Hydrolysis of ureas Layer-by-layer assemblies Long term stability Polyelectrolyte multilayer Reduced graphene oxides Weak polyelectrolytes Field effect transistors copper ion graphene oxide polyelectrolyte polyethyleneimine urease graphite immobilized enzyme oxide polyelectrolyte urea urease aqueous solution Article biosensor catalysis enzyme analysis field effect transistor hydrolysis limit of detection pH measurement surface property blood Canavalia chemistry devices enzymology equipment design evaluation study genetic procedures human transistor Biosensing Techniques Canavalia Enzymes, Immobilized Equipment Design Graphite Humans Limit of Detection Oxides Polyelectrolytes Transistors, Electronic Urea Urease Battaglini, Fernando Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications |
| topic_facet |
Biosensors Chemical sensors Field-effect transistors Graphene Biosensors Chemical sensors Copper Enzymes Graphene Graphene transistors Heavy metals Metabolism pH sensors Polyelectrolytes Solutions Transistors Urea Bio-sensor platforms Biosensing applications Hydrolysis of ureas Layer-by-layer assemblies Long term stability Polyelectrolyte multilayer Reduced graphene oxides Weak polyelectrolytes Field effect transistors copper ion graphene oxide polyelectrolyte polyethyleneimine urease graphite immobilized enzyme oxide polyelectrolyte urea urease aqueous solution Article biosensor catalysis enzyme analysis field effect transistor hydrolysis limit of detection pH measurement surface property blood Canavalia chemistry devices enzymology equipment design evaluation study genetic procedures human transistor Biosensing Techniques Canavalia Enzymes, Immobilized Equipment Design Graphite Humans Limit of Detection Oxides Polyelectrolytes Transistors, Electronic Urea Urease |
| description |
We present the construction of layer-by-layer (LbL) assemblies of polyethylenimine and urease onto reduced-graphene-oxide based field-effect transistors (rGO FETs) for the detection of urea. This versatile biosensor platform simultaneously exploits the pH dependency of liquid-gated graphene-based transistors and the change in the local pH produced by the catalyzed hydrolysis of urea. The use of an interdigitated microchannel resulted in transistors displaying low noise, high pH sensitivity (20.3 µA/pH) and transconductance values up to 800 µS. The modification of rGO FETs with a weak polyelectrolyte improved the pH response because of its transducing properties by electrostatic gating effects. In the presence of urea, the urease-modified rGO FETs showed a shift in the Dirac point due to the change in the local pH close to the graphene surface. Markedly, these devices operated at very low voltages (less than 500 mV) and were able to monitor urea in the range of 1–1000 µm, with a limit of detection (LOD) down to 1 µm, fast response and good long-term stability. The urea-response of the transistors was enhanced by increasing the number of bilayers due to the increment of the enzyme surface coverage onto the channel. Moreover, quantification of the heavy metal Cu 2+ (with a LOD down to 10 nM) was performed in aqueous solution by taking advantage of the urease specific inhibition. © 2016 The Authors |
| author |
Battaglini, Fernando |
| author_facet |
Battaglini, Fernando |
| author_sort |
Battaglini, Fernando |
| title |
Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications |
| title_short |
Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications |
| title_full |
Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications |
| title_fullStr |
Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications |
| title_full_unstemmed |
Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications |
| title_sort |
enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09565663_v92_n_p661_Piccinini http://hdl.handle.net/20.500.12110/paper_09565663_v92_n_p661_Piccinini |
| work_keys_str_mv |
AT battaglinifernando enzymepolyelectrolytemultilayerassembliesonreducedgrapheneoxidefieldeffecttransistorsforbiosensingapplications |
| _version_ |
1768542515048218624 |