Polyporus s. str. in southern South America: Isoenzyme analysis

Forty-two dikaryotic and 42 monokaryotic isolates, and 34 pairings were examined by horizontal polyacrylamide gel electrophoresis (PAGE) for six enzymatic activities, viz. EST, 6PGD, IDH, MDH, SHDH and SOD. 44 bands were analysed. Numerical analysis of the isoenzymatic patterns was undertaken and co...

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Detalles Bibliográficos
Autores principales: Borges da Silveira, Rosa Mara, Saidman, Beatriz Ofelia
Publicado: 2003
Materias:
enzyme
fungus
Fungi
Polyporaceae
Polyporus
Polyporus ciliatus
Polyporus tenuiculus
Polyporus tucumanensis
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09537562_v107_n5_p597_BorgesDaSilveira
http://hdl.handle.net/20.500.12110/paper_09537562_v107_n5_p597_BorgesDaSilveira
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09537562_v107_n5_p597_BorgesDaSilveira
record_format dspace
spelling paper:paper_09537562_v107_n5_p597_BorgesDaSilveira2023-06-08T15:55:25Z Polyporus s. str. in southern South America: Isoenzyme analysis Borges da Silveira, Rosa Mara Saidman, Beatriz Ofelia enzyme fungus Fungi Polyporaceae Polyporus Polyporus Polyporus ciliatus Polyporus ciliatus Polyporus tenuiculus Polyporus tenuiculus Polyporus tucumanensis Forty-two dikaryotic and 42 monokaryotic isolates, and 34 pairings were examined by horizontal polyacrylamide gel electrophoresis (PAGE) for six enzymatic activities, viz. EST, 6PGD, IDH, MDH, SHDH and SOD. 44 bands were analysed. Numerical analysis of the isoenzymatic patterns was undertaken and compared with those from morphological characters. The analysis of six enzymatic systems showed the existence of four monomorphic systems (IDH, MDH, SHDH and SOD). The sterease system (EST) appears to be polymorphic in Polyporus ciliatus and in populations of P. tenuiculus from Argentina, being monomorphic in the remaining species studied. The 6PGD system is polymorphic in P. tucumanensis and monomorphic in the other species. Predominance of monomorphic enzymes and a clear distribution of the electromorphs among the species, indicates that isoenzymatic analysis is a good taxonomic tool within Polyporus. The low intraspecific variability allowed the use of interspecific differences to separate species. Numerical analysis showed a good correlation between morphological and molecular characters. In the isoenzymatic phenogram the similarity index is high only among very close species, showing a stressed separation of species. Fil:Borges Da Silveira, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Saidman, B.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09537562_v107_n5_p597_BorgesDaSilveira http://hdl.handle.net/20.500.12110/paper_09537562_v107_n5_p597_BorgesDaSilveira
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic enzyme
fungus
Fungi
Polyporaceae
Polyporus
Polyporus
Polyporus ciliatus
Polyporus ciliatus
Polyporus tenuiculus
Polyporus tenuiculus
Polyporus tucumanensis
spellingShingle enzyme
fungus
Fungi
Polyporaceae
Polyporus
Polyporus
Polyporus ciliatus
Polyporus ciliatus
Polyporus tenuiculus
Polyporus tenuiculus
Polyporus tucumanensis
Borges da Silveira, Rosa Mara
Saidman, Beatriz Ofelia
Polyporus s. str. in southern South America: Isoenzyme analysis
topic_facet enzyme
fungus
Fungi
Polyporaceae
Polyporus
Polyporus
Polyporus ciliatus
Polyporus ciliatus
Polyporus tenuiculus
Polyporus tenuiculus
Polyporus tucumanensis
description Forty-two dikaryotic and 42 monokaryotic isolates, and 34 pairings were examined by horizontal polyacrylamide gel electrophoresis (PAGE) for six enzymatic activities, viz. EST, 6PGD, IDH, MDH, SHDH and SOD. 44 bands were analysed. Numerical analysis of the isoenzymatic patterns was undertaken and compared with those from morphological characters. The analysis of six enzymatic systems showed the existence of four monomorphic systems (IDH, MDH, SHDH and SOD). The sterease system (EST) appears to be polymorphic in Polyporus ciliatus and in populations of P. tenuiculus from Argentina, being monomorphic in the remaining species studied. The 6PGD system is polymorphic in P. tucumanensis and monomorphic in the other species. Predominance of monomorphic enzymes and a clear distribution of the electromorphs among the species, indicates that isoenzymatic analysis is a good taxonomic tool within Polyporus. The low intraspecific variability allowed the use of interspecific differences to separate species. Numerical analysis showed a good correlation between morphological and molecular characters. In the isoenzymatic phenogram the similarity index is high only among very close species, showing a stressed separation of species.
author Borges da Silveira, Rosa Mara
Saidman, Beatriz Ofelia
author_facet Borges da Silveira, Rosa Mara
Saidman, Beatriz Ofelia
author_sort Borges da Silveira, Rosa Mara
title Polyporus s. str. in southern South America: Isoenzyme analysis
title_short Polyporus s. str. in southern South America: Isoenzyme analysis
title_full Polyporus s. str. in southern South America: Isoenzyme analysis
title_fullStr Polyporus s. str. in southern South America: Isoenzyme analysis
title_full_unstemmed Polyporus s. str. in southern South America: Isoenzyme analysis
title_sort polyporus s. str. in southern south america: isoenzyme analysis
publishDate 2003
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09537562_v107_n5_p597_BorgesDaSilveira
http://hdl.handle.net/20.500.12110/paper_09537562_v107_n5_p597_BorgesDaSilveira
work_keys_str_mv AT borgesdasilveirarosamara polyporussstrinsouthernsouthamericaisoenzymeanalysis
AT saidmanbeatrizofelia polyporussstrinsouthernsouthamericaisoenzymeanalysis
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