Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide
The mechanisms of the teratogenic effects of maternal alcohol consumption remain unclear. The aim of the present work was to study the organogenic PGE2 levels and the modulation of PGE2 levels by NO after periconceptional alcohol ingestion. Female mice were intoxicated with a 10% ethanol in drinking...
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2007
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v76_n3_p141_Cebral http://hdl.handle.net/20.500.12110/paper_09523278_v76_n3_p141_Cebral |
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paper:paper_09523278_v76_n3_p141_Cebral2023-06-08T15:55:02Z Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide alcohol drinking water n(g) methylarginine nitric oxide nitric oxide donor nitric oxide synthase inhibitor prostaglandin E alcohol consumption alcohol intoxication animal experiment animal tissue article controlled study embryo embryo development embryo resorption embryopathy female gestation period mouse neural tube neural tube defect nonhuman organogenesis pregnancy priority journal prostaglandin release radioimmunoassay Alcohol Drinking Animals Body Weight Central Nervous System Depressants Embryo Embryo Implantation Embryonic Development Enzyme Inhibitors Ethanol Female Mice Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organogenesis Pregnancy Prostaglandins E Radioimmunoassay Mus The mechanisms of the teratogenic effects of maternal alcohol consumption remain unclear. The aim of the present work was to study the organogenic PGE2 levels and the modulation of PGE2 levels by NO after periconceptional alcohol ingestion. Female mice were intoxicated with a 10% ethanol in drinking water before pregnancy and up to day 10 of gestation. The PGE2 released from organogenic embryos was measured by radio immunoassay following incubation with or without the addition of either a NO donor or a NO synthase (NOS) inhibitor. In the ethanol-treated females, we found increased percentages of retarded embryos, associated with a significantly elevated resorption rate (p < 0.05), very high quantities of morphologically abnormal E.10 embryos (p < 0.001) and significantly increased PGE2 release, as compared to the embryo parameters of control females. While in the control-derived E.10 embryos the NO donor produced significantly increased PGE2 release, in the ethanol-derived embryos decreased quantities of PGE2 were observed. L-NMMA inhibited PGE2 release in both control and ethanol-derived embryos at different concentrations, whereas it decreased PGE2 content in controls but not in ethanol-derived embryos. The periconceptional alcohol ingestion produced excessive PGE2 release, decreased PGE2 content and disruption of the regulatory NO-PGE2 pathways. These PGs alterations may be related to delayed organogenesis and abnormal neural tube development after chronic periconceptional consumption of alcohol. © 2007. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v76_n3_p141_Cebral http://hdl.handle.net/20.500.12110/paper_09523278_v76_n3_p141_Cebral |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alcohol drinking water n(g) methylarginine nitric oxide nitric oxide donor nitric oxide synthase inhibitor prostaglandin E alcohol consumption alcohol intoxication animal experiment animal tissue article controlled study embryo embryo development embryo resorption embryopathy female gestation period mouse neural tube neural tube defect nonhuman organogenesis pregnancy priority journal prostaglandin release radioimmunoassay Alcohol Drinking Animals Body Weight Central Nervous System Depressants Embryo Embryo Implantation Embryonic Development Enzyme Inhibitors Ethanol Female Mice Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organogenesis Pregnancy Prostaglandins E Radioimmunoassay Mus |
| spellingShingle |
alcohol drinking water n(g) methylarginine nitric oxide nitric oxide donor nitric oxide synthase inhibitor prostaglandin E alcohol consumption alcohol intoxication animal experiment animal tissue article controlled study embryo embryo development embryo resorption embryopathy female gestation period mouse neural tube neural tube defect nonhuman organogenesis pregnancy priority journal prostaglandin release radioimmunoassay Alcohol Drinking Animals Body Weight Central Nervous System Depressants Embryo Embryo Implantation Embryonic Development Enzyme Inhibitors Ethanol Female Mice Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organogenesis Pregnancy Prostaglandins E Radioimmunoassay Mus Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide |
| topic_facet |
alcohol drinking water n(g) methylarginine nitric oxide nitric oxide donor nitric oxide synthase inhibitor prostaglandin E alcohol consumption alcohol intoxication animal experiment animal tissue article controlled study embryo embryo development embryo resorption embryopathy female gestation period mouse neural tube neural tube defect nonhuman organogenesis pregnancy priority journal prostaglandin release radioimmunoassay Alcohol Drinking Animals Body Weight Central Nervous System Depressants Embryo Embryo Implantation Embryonic Development Enzyme Inhibitors Ethanol Female Mice Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organogenesis Pregnancy Prostaglandins E Radioimmunoassay Mus |
| description |
The mechanisms of the teratogenic effects of maternal alcohol consumption remain unclear. The aim of the present work was to study the organogenic PGE2 levels and the modulation of PGE2 levels by NO after periconceptional alcohol ingestion. Female mice were intoxicated with a 10% ethanol in drinking water before pregnancy and up to day 10 of gestation. The PGE2 released from organogenic embryos was measured by radio immunoassay following incubation with or without the addition of either a NO donor or a NO synthase (NOS) inhibitor. In the ethanol-treated females, we found increased percentages of retarded embryos, associated with a significantly elevated resorption rate (p < 0.05), very high quantities of morphologically abnormal E.10 embryos (p < 0.001) and significantly increased PGE2 release, as compared to the embryo parameters of control females. While in the control-derived E.10 embryos the NO donor produced significantly increased PGE2 release, in the ethanol-derived embryos decreased quantities of PGE2 were observed. L-NMMA inhibited PGE2 release in both control and ethanol-derived embryos at different concentrations, whereas it decreased PGE2 content in controls but not in ethanol-derived embryos. The periconceptional alcohol ingestion produced excessive PGE2 release, decreased PGE2 content and disruption of the regulatory NO-PGE2 pathways. These PGs alterations may be related to delayed organogenesis and abnormal neural tube development after chronic periconceptional consumption of alcohol. © 2007. |
| title |
Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide |
| title_short |
Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide |
| title_full |
Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide |
| title_fullStr |
Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide |
| title_full_unstemmed |
Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide |
| title_sort |
periconceptional alcohol consumption-induced changes in embryonic prostaglandin e levels in mouse organogenesis: modulation by nitric oxide |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v76_n3_p141_Cebral http://hdl.handle.net/20.500.12110/paper_09523278_v76_n3_p141_Cebral |
| _version_ |
1768545198760001536 |