A novel enhancing effect of platelet activating factor (PAF) on glucose oxidation in uteri from pregnant rats. Participation of prostaglandins and leukotrienes
The effects of platelet activating factor (PAF) on glucose oxidation in uterine strips isolated from rats in the 4 th and 5 th day of pregnancy, were explored. PAF, at a concentration of 10-10 and 10-8 M, augmented significantly the generation of 14CO2 from labelled glucose in uteri from pregnant ra...
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1990
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v40_n1_p79_Gonzalez http://hdl.handle.net/20.500.12110/paper_09523278_v40_n1_p79_Gonzalez |
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paper:paper_09523278_v40_n1_p79_Gonzalez2023-06-08T15:55:01Z A novel enhancing effect of platelet activating factor (PAF) on glucose oxidation in uteri from pregnant rats. Participation of prostaglandins and leukotrienes 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid ginkgolide b indometacin leukotriene c4 nordihydroguaiaretic acid prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha radioisotope thrombocyte activating factor animal cell article female glucose metabolism nonhuman pregnancy priority journal rat thrombocyte tissue culture uterus Animal Carbon Monoxide Chromones Female Glucose Indomethacin Leukotrienes Nordihydroguaiaretic Acid Platelet Activating Factor Pregnancy Pregnancy, Animal Prostaglandins Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus Animalia The effects of platelet activating factor (PAF) on glucose oxidation in uterine strips isolated from rats in the 4 th and 5 th day of pregnancy, were explored. PAF, at a concentration of 10-10 and 10-8 M, augmented significantly the generation of 14CO2 from labelled glucose in uteri from pregnant rats in the 4 th day of pregnancy. When the tissue was obtained from 5 days pregnant rats, the addition of PAF at 10-8 increased significantly more than PAF at 10-10 M the metabolism of glucose. On the other hand, PAF at 10-8 M failed to alter the uterine basal production of 14CO2 from labelled glucose in animals at estrus. BN52021, a specific PAF antagonist employed at 10-5 M, blocked completely the action of PAF in the pregnant rat uterus. PGE1, PGE2 and PGF2α enhanced significantly the formation of 14CO2 from labelled glucose in uteri from 5 days pregnant rats. Indomethacin, a well known inhibitor of prostaglandin synthesis, did not alter the basal glucose metabolism in uteri from 5 days pregnant rats, but antagonized completely the stimulating action of PAF on 14CO2 production from labelled glucose an effect that was partially reverted by the addition of PGE1, PGE2 or PGF2α (10-7 M). Furthermore, nordihydroguaiaretic acid (NDHGA), a specific inhibitor of 5-lipoxygenase at 10-5 M, as well as FPL-55712, an antagonist of leukotrienes (LTs), at the same concentration, blocked the action of PAF on the metabolism of glucose. The action of NDHGA was partially counteracted by the addition of LTC4 at 10-7 M. Considering all these results, we suggest that PAF stimulates indirectly glucose metabolism via the "de novo" synthesis of PGs anb LTs, probably by the activation of phospholipase A2 (PLA2). As far as we know the present report represent the first description of a stimulating action of PAF on glucose metabolism in pregnant rat uteri. © 1990. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v40_n1_p79_Gonzalez http://hdl.handle.net/20.500.12110/paper_09523278_v40_n1_p79_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid ginkgolide b indometacin leukotriene c4 nordihydroguaiaretic acid prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha radioisotope thrombocyte activating factor animal cell article female glucose metabolism nonhuman pregnancy priority journal rat thrombocyte tissue culture uterus Animal Carbon Monoxide Chromones Female Glucose Indomethacin Leukotrienes Nordihydroguaiaretic Acid Platelet Activating Factor Pregnancy Pregnancy, Animal Prostaglandins Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus Animalia |
| spellingShingle |
7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid ginkgolide b indometacin leukotriene c4 nordihydroguaiaretic acid prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha radioisotope thrombocyte activating factor animal cell article female glucose metabolism nonhuman pregnancy priority journal rat thrombocyte tissue culture uterus Animal Carbon Monoxide Chromones Female Glucose Indomethacin Leukotrienes Nordihydroguaiaretic Acid Platelet Activating Factor Pregnancy Pregnancy, Animal Prostaglandins Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus Animalia A novel enhancing effect of platelet activating factor (PAF) on glucose oxidation in uteri from pregnant rats. Participation of prostaglandins and leukotrienes |
| topic_facet |
7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid ginkgolide b indometacin leukotriene c4 nordihydroguaiaretic acid prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha radioisotope thrombocyte activating factor animal cell article female glucose metabolism nonhuman pregnancy priority journal rat thrombocyte tissue culture uterus Animal Carbon Monoxide Chromones Female Glucose Indomethacin Leukotrienes Nordihydroguaiaretic Acid Platelet Activating Factor Pregnancy Pregnancy, Animal Prostaglandins Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus Animalia |
| description |
The effects of platelet activating factor (PAF) on glucose oxidation in uterine strips isolated from rats in the 4 th and 5 th day of pregnancy, were explored. PAF, at a concentration of 10-10 and 10-8 M, augmented significantly the generation of 14CO2 from labelled glucose in uteri from pregnant rats in the 4 th day of pregnancy. When the tissue was obtained from 5 days pregnant rats, the addition of PAF at 10-8 increased significantly more than PAF at 10-10 M the metabolism of glucose. On the other hand, PAF at 10-8 M failed to alter the uterine basal production of 14CO2 from labelled glucose in animals at estrus. BN52021, a specific PAF antagonist employed at 10-5 M, blocked completely the action of PAF in the pregnant rat uterus. PGE1, PGE2 and PGF2α enhanced significantly the formation of 14CO2 from labelled glucose in uteri from 5 days pregnant rats. Indomethacin, a well known inhibitor of prostaglandin synthesis, did not alter the basal glucose metabolism in uteri from 5 days pregnant rats, but antagonized completely the stimulating action of PAF on 14CO2 production from labelled glucose an effect that was partially reverted by the addition of PGE1, PGE2 or PGF2α (10-7 M). Furthermore, nordihydroguaiaretic acid (NDHGA), a specific inhibitor of 5-lipoxygenase at 10-5 M, as well as FPL-55712, an antagonist of leukotrienes (LTs), at the same concentration, blocked the action of PAF on the metabolism of glucose. The action of NDHGA was partially counteracted by the addition of LTC4 at 10-7 M. Considering all these results, we suggest that PAF stimulates indirectly glucose metabolism via the "de novo" synthesis of PGs anb LTs, probably by the activation of phospholipase A2 (PLA2). As far as we know the present report represent the first description of a stimulating action of PAF on glucose metabolism in pregnant rat uteri. © 1990. |
| title |
A novel enhancing effect of platelet activating factor (PAF) on glucose oxidation in uteri from pregnant rats. Participation of prostaglandins and leukotrienes |
| title_short |
A novel enhancing effect of platelet activating factor (PAF) on glucose oxidation in uteri from pregnant rats. Participation of prostaglandins and leukotrienes |
| title_full |
A novel enhancing effect of platelet activating factor (PAF) on glucose oxidation in uteri from pregnant rats. Participation of prostaglandins and leukotrienes |
| title_fullStr |
A novel enhancing effect of platelet activating factor (PAF) on glucose oxidation in uteri from pregnant rats. Participation of prostaglandins and leukotrienes |
| title_full_unstemmed |
A novel enhancing effect of platelet activating factor (PAF) on glucose oxidation in uteri from pregnant rats. Participation of prostaglandins and leukotrienes |
| title_sort |
novel enhancing effect of platelet activating factor (paf) on glucose oxidation in uteri from pregnant rats. participation of prostaglandins and leukotrienes |
| publishDate |
1990 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v40_n1_p79_Gonzalez http://hdl.handle.net/20.500.12110/paper_09523278_v40_n1_p79_Gonzalez |
| _version_ |
1768543092658405376 |