Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices
The successful analysis by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF MS) of native and hydrolyzed high-methoxylated pectin samples is described. In order to find the optimal conditions for UV-MALDI-TOF MS analysis several experimental vari...
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2007
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v21_n16_p2638_Monge http://hdl.handle.net/20.500.12110/paper_09514198_v21_n16_p2638_Monge |
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paper:paper_09514198_v21_n16_p2638_Monge2023-06-08T15:54:58Z Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices Monge, María Eugenia Negri, Ricardo Martin Kolender, Adriana Andrea Erra Balsells, Rosa 2,5-dihydroxybenzoic acid drug derivative gentisic acid harman harmine pectin unclassified drug article chemistry electrospray mass spectrometry hydroxylation mass spectrometry methodology nuclear magnetic resonance spectroscopy reproducibility sensitivity and specificity ultraviolet spectrophotometry Gentisates Harmine Hydroxylation Magnetic Resonance Spectroscopy Pectins Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet The successful analysis by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF MS) of native and hydrolyzed high-methoxylated pectin samples is described. In order to find the optimal conditions for UV-MALDI-TOF MS analysis several experimental variables were studied such as: different UV-MALDI matrices (nor-harmane, 2,5-dihydroxybenzoic acid), sample preparation methods (mixture, sandwich), inorganic salt addition (doping salts, NaCl, KCl, NH4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOF MS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. Copyright © 2007 John Wiley & Sons, Ltd. Fil:Monge, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Negri, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kolender, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v21_n16_p2638_Monge http://hdl.handle.net/20.500.12110/paper_09514198_v21_n16_p2638_Monge |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2,5-dihydroxybenzoic acid drug derivative gentisic acid harman harmine pectin unclassified drug article chemistry electrospray mass spectrometry hydroxylation mass spectrometry methodology nuclear magnetic resonance spectroscopy reproducibility sensitivity and specificity ultraviolet spectrophotometry Gentisates Harmine Hydroxylation Magnetic Resonance Spectroscopy Pectins Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet |
| spellingShingle |
2,5-dihydroxybenzoic acid drug derivative gentisic acid harman harmine pectin unclassified drug article chemistry electrospray mass spectrometry hydroxylation mass spectrometry methodology nuclear magnetic resonance spectroscopy reproducibility sensitivity and specificity ultraviolet spectrophotometry Gentisates Harmine Hydroxylation Magnetic Resonance Spectroscopy Pectins Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Monge, María Eugenia Negri, Ricardo Martin Kolender, Adriana Andrea Erra Balsells, Rosa Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices |
| topic_facet |
2,5-dihydroxybenzoic acid drug derivative gentisic acid harman harmine pectin unclassified drug article chemistry electrospray mass spectrometry hydroxylation mass spectrometry methodology nuclear magnetic resonance spectroscopy reproducibility sensitivity and specificity ultraviolet spectrophotometry Gentisates Harmine Hydroxylation Magnetic Resonance Spectroscopy Pectins Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet |
| description |
The successful analysis by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF MS) of native and hydrolyzed high-methoxylated pectin samples is described. In order to find the optimal conditions for UV-MALDI-TOF MS analysis several experimental variables were studied such as: different UV-MALDI matrices (nor-harmane, 2,5-dihydroxybenzoic acid), sample preparation methods (mixture, sandwich), inorganic salt addition (doping salts, NaCl, KCl, NH4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOF MS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. Copyright © 2007 John Wiley & Sons, Ltd. |
| author |
Monge, María Eugenia Negri, Ricardo Martin Kolender, Adriana Andrea Erra Balsells, Rosa |
| author_facet |
Monge, María Eugenia Negri, Ricardo Martin Kolender, Adriana Andrea Erra Balsells, Rosa |
| author_sort |
Monge, María Eugenia |
| title |
Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices |
| title_short |
Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices |
| title_full |
Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices |
| title_fullStr |
Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices |
| title_full_unstemmed |
Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices |
| title_sort |
structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as uv-maldi matrices |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v21_n16_p2638_Monge http://hdl.handle.net/20.500.12110/paper_09514198_v21_n16_p2638_Monge |
| work_keys_str_mv |
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