Evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
In an effort to gain an understanding of the processes governing ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI), direct comparison was made of the mass spectra of proteins, carbohydrates and synthetic polymers (polyethylene glycol, polyester and polyamide) by using pyridylindoles...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v15_n23_p2354_Nonami http://hdl.handle.net/20.500.12110/paper_09514198_v15_n23_p2354_Nonami |
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paper:paper_09514198_v15_n23_p2354_Nonami2023-06-08T15:54:57Z Evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Erra Balsells, Rosa 2,5-dihydroxybenzoic acid bipyridine derivative carbohydrate carboline derivative cyclodextrin gentisic acid harman harmine hydrogen hydroxybenzoic acid derivative indole derivative maneb nitrogen polymer protein pyridine derivative pyrifenox mancozeb pyrifenox-mancozeb zineb article carbohydrate analysis chemistry crystallization desorption drug combination ionization mass spectrometry methodology protein analysis protein structure ultraviolet radiation ultraviolet spectrophotometry Carbohydrates Crystallization Drug Combinations Gentisates Harmine Hydroxybenzoic Acids Indoles Maneb Polymers Proteins Pyridines Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Zineb In an effort to gain an understanding of the processes governing ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI), direct comparison was made of the mass spectra of proteins, carbohydrates and synthetic polymers (polyethylene glycol, polyester and polyamide) by using pyridylindoles, pyridoindoles and pyridylpyridoindoles as UV (337 nm)-MALDI-TOFMS matrices in positive and negative ion mode. In order to study the combined effect of the indole N-H and the pyridine nitrogen of the MALDI matrix on the desorption/ionization process in MALDI, compounds were selected that include either or both of these functions in their structure. Within the compounds studied only those that possess simultaneously both functions in a 1,4-relation behave as very good matrices for proteins. These compounds also work as matrices for some carbohydrates and synthetic polymers used as analytes in the present study. Some of the compounds were also found to be useful for the post-source decay (PSD) analysis of cyclodextrins in positive and negative ion mode. In several cases we also examined the matrix behavior of the corresponding N-methylindole derivatives. Copyright © 2001 John Wiley & Sons, Ltd. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v15_n23_p2354_Nonami http://hdl.handle.net/20.500.12110/paper_09514198_v15_n23_p2354_Nonami |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
2,5-dihydroxybenzoic acid bipyridine derivative carbohydrate carboline derivative cyclodextrin gentisic acid harman harmine hydrogen hydroxybenzoic acid derivative indole derivative maneb nitrogen polymer protein pyridine derivative pyrifenox mancozeb pyrifenox-mancozeb zineb article carbohydrate analysis chemistry crystallization desorption drug combination ionization mass spectrometry methodology protein analysis protein structure ultraviolet radiation ultraviolet spectrophotometry Carbohydrates Crystallization Drug Combinations Gentisates Harmine Hydroxybenzoic Acids Indoles Maneb Polymers Proteins Pyridines Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Zineb |
spellingShingle |
2,5-dihydroxybenzoic acid bipyridine derivative carbohydrate carboline derivative cyclodextrin gentisic acid harman harmine hydrogen hydroxybenzoic acid derivative indole derivative maneb nitrogen polymer protein pyridine derivative pyrifenox mancozeb pyrifenox-mancozeb zineb article carbohydrate analysis chemistry crystallization desorption drug combination ionization mass spectrometry methodology protein analysis protein structure ultraviolet radiation ultraviolet spectrophotometry Carbohydrates Crystallization Drug Combinations Gentisates Harmine Hydroxybenzoic Acids Indoles Maneb Polymers Proteins Pyridines Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Zineb Erra Balsells, Rosa Evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
topic_facet |
2,5-dihydroxybenzoic acid bipyridine derivative carbohydrate carboline derivative cyclodextrin gentisic acid harman harmine hydrogen hydroxybenzoic acid derivative indole derivative maneb nitrogen polymer protein pyridine derivative pyrifenox mancozeb pyrifenox-mancozeb zineb article carbohydrate analysis chemistry crystallization desorption drug combination ionization mass spectrometry methodology protein analysis protein structure ultraviolet radiation ultraviolet spectrophotometry Carbohydrates Crystallization Drug Combinations Gentisates Harmine Hydroxybenzoic Acids Indoles Maneb Polymers Proteins Pyridines Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Ultraviolet Zineb |
description |
In an effort to gain an understanding of the processes governing ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI), direct comparison was made of the mass spectra of proteins, carbohydrates and synthetic polymers (polyethylene glycol, polyester and polyamide) by using pyridylindoles, pyridoindoles and pyridylpyridoindoles as UV (337 nm)-MALDI-TOFMS matrices in positive and negative ion mode. In order to study the combined effect of the indole N-H and the pyridine nitrogen of the MALDI matrix on the desorption/ionization process in MALDI, compounds were selected that include either or both of these functions in their structure. Within the compounds studied only those that possess simultaneously both functions in a 1,4-relation behave as very good matrices for proteins. These compounds also work as matrices for some carbohydrates and synthetic polymers used as analytes in the present study. Some of the compounds were also found to be useful for the post-source decay (PSD) analysis of cyclodextrins in positive and negative ion mode. In several cases we also examined the matrix behavior of the corresponding N-methylindole derivatives. Copyright © 2001 John Wiley & Sons, Ltd. |
author |
Erra Balsells, Rosa |
author_facet |
Erra Balsells, Rosa |
author_sort |
Erra Balsells, Rosa |
title |
Evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_short |
Evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_full |
Evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_fullStr |
Evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_full_unstemmed |
Evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
title_sort |
evaluation of pyridoindoles, pyridylindoles and pyridylpyridoindoles as matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
publishDate |
2001 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v15_n23_p2354_Nonami http://hdl.handle.net/20.500.12110/paper_09514198_v15_n23_p2354_Nonami |
work_keys_str_mv |
AT errabalsellsrosa evaluationofpyridoindolespyridylindolesandpyridylpyridoindolesasmatricesforultravioletmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry |
_version_ |
1768546500453859328 |