Wnt-regulated dynamics of positional information in zebrafish somitogenesis
How signaling gradients supply positional information in a field of moving cells is an unsolved question in patterning and morphogenesis. Here, we ask how a Wnt signaling gradient regulates the dynamics of a wavefront of cellular change in a flow of cells during somitogenesis. Using time-controlled...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09501991_v141_n6_p1381_Bajard http://hdl.handle.net/20.500.12110/paper_09501991_v141_n6_p1381_Bajard |
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paper:paper_09501991_v141_n6_p1381_Bajard |
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paper:paper_09501991_v141_n6_p1381_Bajard2023-06-08T15:54:24Z Wnt-regulated dynamics of positional information in zebrafish somitogenesis Embryonic elongation Fgf signaling Segmentation clock Signal gradient Time-lapse microscopy Wnt signaling Wnt protein animal experiment article controlled study embryo flow rate fluorescence gene expression heat shock immunohistochemistry in situ hybridization morphogenesis nonhuman notochord oscillation priority journal regulatory mechanism somite somitogenesis Wnt signaling pathway zebra fish Embryonic elongation Fgf signaling Segmentation clock Signal gradient Time-lapse microscopy Wnt signaling Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors Body Patterning Fibroblast Growth Factors Gene Expression Regulation, Developmental Heat-Shock Response Intercellular Signaling Peptides and Proteins Models, Biological Somites T-Box Domain Proteins Wnt Proteins Wnt Signaling Pathway Zebrafish Zebrafish Proteins How signaling gradients supply positional information in a field of moving cells is an unsolved question in patterning and morphogenesis. Here, we ask how a Wnt signaling gradient regulates the dynamics of a wavefront of cellular change in a flow of cells during somitogenesis. Using time-controlled perturbations of Wnt signaling in the zebrafish embryo, we changed segment length without altering the rate of somite formation or embryonic elongation. This result implies specific Wnt regulation of the wavefront velocity. The observed Wnt signaling gradient dynamics and timing of downstream events support a model for wavefront regulation in which cell flow plays a dominant role in transporting positional information. © 2014. Published by The Company of Biologists Ltd. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09501991_v141_n6_p1381_Bajard http://hdl.handle.net/20.500.12110/paper_09501991_v141_n6_p1381_Bajard |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Embryonic elongation Fgf signaling Segmentation clock Signal gradient Time-lapse microscopy Wnt signaling Wnt protein animal experiment article controlled study embryo flow rate fluorescence gene expression heat shock immunohistochemistry in situ hybridization morphogenesis nonhuman notochord oscillation priority journal regulatory mechanism somite somitogenesis Wnt signaling pathway zebra fish Embryonic elongation Fgf signaling Segmentation clock Signal gradient Time-lapse microscopy Wnt signaling Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors Body Patterning Fibroblast Growth Factors Gene Expression Regulation, Developmental Heat-Shock Response Intercellular Signaling Peptides and Proteins Models, Biological Somites T-Box Domain Proteins Wnt Proteins Wnt Signaling Pathway Zebrafish Zebrafish Proteins |
| spellingShingle |
Embryonic elongation Fgf signaling Segmentation clock Signal gradient Time-lapse microscopy Wnt signaling Wnt protein animal experiment article controlled study embryo flow rate fluorescence gene expression heat shock immunohistochemistry in situ hybridization morphogenesis nonhuman notochord oscillation priority journal regulatory mechanism somite somitogenesis Wnt signaling pathway zebra fish Embryonic elongation Fgf signaling Segmentation clock Signal gradient Time-lapse microscopy Wnt signaling Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors Body Patterning Fibroblast Growth Factors Gene Expression Regulation, Developmental Heat-Shock Response Intercellular Signaling Peptides and Proteins Models, Biological Somites T-Box Domain Proteins Wnt Proteins Wnt Signaling Pathway Zebrafish Zebrafish Proteins Wnt-regulated dynamics of positional information in zebrafish somitogenesis |
| topic_facet |
Embryonic elongation Fgf signaling Segmentation clock Signal gradient Time-lapse microscopy Wnt signaling Wnt protein animal experiment article controlled study embryo flow rate fluorescence gene expression heat shock immunohistochemistry in situ hybridization morphogenesis nonhuman notochord oscillation priority journal regulatory mechanism somite somitogenesis Wnt signaling pathway zebra fish Embryonic elongation Fgf signaling Segmentation clock Signal gradient Time-lapse microscopy Wnt signaling Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors Body Patterning Fibroblast Growth Factors Gene Expression Regulation, Developmental Heat-Shock Response Intercellular Signaling Peptides and Proteins Models, Biological Somites T-Box Domain Proteins Wnt Proteins Wnt Signaling Pathway Zebrafish Zebrafish Proteins |
| description |
How signaling gradients supply positional information in a field of moving cells is an unsolved question in patterning and morphogenesis. Here, we ask how a Wnt signaling gradient regulates the dynamics of a wavefront of cellular change in a flow of cells during somitogenesis. Using time-controlled perturbations of Wnt signaling in the zebrafish embryo, we changed segment length without altering the rate of somite formation or embryonic elongation. This result implies specific Wnt regulation of the wavefront velocity. The observed Wnt signaling gradient dynamics and timing of downstream events support a model for wavefront regulation in which cell flow plays a dominant role in transporting positional information. © 2014. Published by The Company of Biologists Ltd. |
| title |
Wnt-regulated dynamics of positional information in zebrafish somitogenesis |
| title_short |
Wnt-regulated dynamics of positional information in zebrafish somitogenesis |
| title_full |
Wnt-regulated dynamics of positional information in zebrafish somitogenesis |
| title_fullStr |
Wnt-regulated dynamics of positional information in zebrafish somitogenesis |
| title_full_unstemmed |
Wnt-regulated dynamics of positional information in zebrafish somitogenesis |
| title_sort |
wnt-regulated dynamics of positional information in zebrafish somitogenesis |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09501991_v141_n6_p1381_Bajard http://hdl.handle.net/20.500.12110/paper_09501991_v141_n6_p1381_Bajard |
| _version_ |
1768546030465318912 |