Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis

A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deaminase. The chromatographic behaviour of both proteins wa...

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Detalles Bibliográficos
Autores principales: Rossetti, María Victoria, Juknat, Adela Ana, Batlle, Alcira María del Carmen
Publicado: 1989
Materias:
Euglena gracilis
Particulate
Porphobilinogen-Deaminase
Porphyrins
Soluble
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v44_n7-8_p578_Rossetti
http://hdl.handle.net/20.500.12110/paper_09395075_v44_n7-8_p578_Rossetti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09395075_v44_n7-8_p578_Rossetti
record_format dspace
spelling paper:paper_09395075_v44_n7-8_p578_Rossetti2023-06-08T15:53:25Z Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis Rossetti, María Victoria Juknat, Adela Ana Batlle, Alcira María del Carmen Euglena gracilis Particulate Porphobilinogen-Deaminase Porphyrins Soluble A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deaminase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deaminase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000. © 1989, Verlag der Zeitschrift. All rights reserved. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Batlle, A.M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v44_n7-8_p578_Rossetti http://hdl.handle.net/20.500.12110/paper_09395075_v44_n7-8_p578_Rossetti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Euglena gracilis
Particulate
Porphobilinogen-Deaminase
Porphyrins
Soluble
spellingShingle Euglena gracilis
Particulate
Porphobilinogen-Deaminase
Porphyrins
Soluble
Rossetti, María Victoria
Juknat, Adela Ana
Batlle, Alcira María del Carmen
Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
topic_facet Euglena gracilis
Particulate
Porphobilinogen-Deaminase
Porphyrins
Soluble
description A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deaminase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deaminase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000. © 1989, Verlag der Zeitschrift. All rights reserved.
author Rossetti, María Victoria
Juknat, Adela Ana
Batlle, Alcira María del Carmen
author_facet Rossetti, María Victoria
Juknat, Adela Ana
Batlle, Alcira María del Carmen
author_sort Rossetti, María Victoria
title Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_short Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_full Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_fullStr Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_full_unstemmed Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis
title_sort soluble and particulate porphobilinogen-deaminase from dark-grown euglena gracilis
publishDate 1989
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v44_n7-8_p578_Rossetti
http://hdl.handle.net/20.500.12110/paper_09395075_v44_n7-8_p578_Rossetti
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AT juknatadelaana solubleandparticulateporphobilinogendeaminasefromdarkgrowneuglenagracilis
AT batllealciramariadelcarmen solubleandparticulateporphobilinogendeaminasefromdarkgrowneuglenagracilis
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