Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina

Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north-western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isol...

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Detalles Bibliográficos
Autor principal: Rodriguero, Marcela Silvina
Publicado: 2016
Materias:
anastomosis group
genetic diversity
ITS sequencing
morphology
Phaseolus vulgaris
virulence
cultivar
disease resistance
fungal disease
fungus
genetic analysis
genetic variation
herb
pathogenicity
Argentina
Fungi
Phaseolus (angiosperm)
Thanatephorus cucumeris
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v164_n11-12_p1054_Spedaletti
http://hdl.handle.net/20.500.12110/paper_09311785_v164_n11-12_p1054_Spedaletti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09311785_v164_n11-12_p1054_Spedaletti
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spelling paper:paper_09311785_v164_n11-12_p1054_Spedaletti2023-06-08T15:52:54Z Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina Rodriguero, Marcela Silvina anastomosis group genetic diversity ITS sequencing morphology Phaseolus vulgaris virulence cultivar disease resistance fungal disease fungus genetic analysis genetic variation herb morphology pathogenicity virulence Argentina Fungi Phaseolus (angiosperm) Phaseolus vulgaris Thanatephorus cucumeris Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north-western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA-ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2-2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north-western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance. © 2016 Blackwell Verlag GmbH Fil:Rodriguero, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v164_n11-12_p1054_Spedaletti http://hdl.handle.net/20.500.12110/paper_09311785_v164_n11-12_p1054_Spedaletti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic anastomosis group
genetic diversity
ITS sequencing
morphology
Phaseolus vulgaris
virulence
cultivar
disease resistance
fungal disease
fungus
genetic analysis
genetic variation
herb
morphology
pathogenicity
virulence
Argentina
Fungi
Phaseolus (angiosperm)
Phaseolus vulgaris
Thanatephorus cucumeris
spellingShingle anastomosis group
genetic diversity
ITS sequencing
morphology
Phaseolus vulgaris
virulence
cultivar
disease resistance
fungal disease
fungus
genetic analysis
genetic variation
herb
morphology
pathogenicity
virulence
Argentina
Fungi
Phaseolus (angiosperm)
Phaseolus vulgaris
Thanatephorus cucumeris
Rodriguero, Marcela Silvina
Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina
topic_facet anastomosis group
genetic diversity
ITS sequencing
morphology
Phaseolus vulgaris
virulence
cultivar
disease resistance
fungal disease
fungus
genetic analysis
genetic variation
herb
morphology
pathogenicity
virulence
Argentina
Fungi
Phaseolus (angiosperm)
Phaseolus vulgaris
Thanatephorus cucumeris
description Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north-western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA-ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2-2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north-western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance. © 2016 Blackwell Verlag GmbH
author Rodriguero, Marcela Silvina
author_facet Rodriguero, Marcela Silvina
author_sort Rodriguero, Marcela Silvina
title Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina
title_short Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina
title_full Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina
title_fullStr Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina
title_full_unstemmed Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina
title_sort genetic characterization and pathogenicity of rhizoctonia solani associated with common bean web blight in the main bean growing area of argentina
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v164_n11-12_p1054_Spedaletti
http://hdl.handle.net/20.500.12110/paper_09311785_v164_n11-12_p1054_Spedaletti
work_keys_str_mv AT rodrigueromarcelasilvina geneticcharacterizationandpathogenicityofrhizoctoniasolaniassociatedwithcommonbeanwebblightinthemainbeangrowingareaofargentina
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