Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review
This review aims to present different aspects related to cruzipain, one of the most important proteins of the etiological agent of Chagas disease that has been extensively studied in the last two decades, including all the particularities of the molecule as well as to highlight its participation in...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09298673_v16_n24_p3174_Duschak http://hdl.handle.net/20.500.12110/paper_09298673_v16_n24_p3174_Duschak |
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paper:paper_09298673_v16_n24_p3174_Duschak2023-06-08T15:52:26Z Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review 2,3,5,6 tetrafluorophenoxymethyl ketone alpha 2 macroglobulin antitrypanosomal agent aryl 4 oxothiazolylhydrazone derivative bisarylacylhydrazide derivative chagasin CpG oligodeoxynucleotide cruzipain cystatin cysteine proteinase cysteine proteinase inhibitor dipeptidyl allyl sulphone derivative dipeptidyl vinyl sulphone derivative hydrolase inhibitor k 11777 k 777 kininogen n methyl pip phenylalanine homo phenylalanine vinyl sulfonylurea phenyl peptide hydrolase inhibitor proteinase inhibitor recombinant cruzipain recombinant enzyme sialic acid unclassified drug virulence factor cruzipain cysteine proteinase cysteine proteinase inhibitor parasite antigen protozoal vaccine catalysis cell invasion Chagas disease chemical composition dog disease drug structure drug targeting enzyme analysis enzyme binding enzyme degradation enzyme mechanism enzyme purification enzyme structure enzyme substrate gene sequence glycosylation heart protection host parasite interaction immune response mammal nonhuman phagocyte protein analysis protein domain protein expression protein polymorphism protozoal infection review structure activity relation structure analysis target cell tissue injury Trypanosoma cruzi vaccine production animal Chagas disease chemistry enzymology human immunology metabolism mouse Trypanosoma cruzi Animals Antigens, Protozoan Chagas Disease Cysteine Endopeptidases Cysteine Proteinase Inhibitors Glycosylation Humans Mice Protozoan Vaccines Trypanosoma cruzi This review aims to present different aspects related to cruzipain, one of the most important proteins of the etiological agent of Chagas disease that has been extensively studied in the last two decades, including all the particularities of the molecule as well as to highlight its participation in multiple relevant functions of the parasite to favour the cell invasion phenomena, to facilitate host tissues proteolytic degradation and to trigger the evasion mechanism from host immune response. Cruzipain has been related with parasite metabolism and identified as both an important candidate for vaccine development and for trypanocidal drug design. We have reported for the first time that this enzyme is a sulfated glycoprotein. Indeed, the sulfated oligosaccharides are main targets for immune responses and are involved in tissue damage in mice immunized in absence of infection contributing to get deeper into the knowledge of the molecule composition and helping to elucidate its role in the infection and/or pathogenesis of the disease. A whole view including all the aspects related to the major cysteine proteinase of Trypanosoma cruzi studied so far including recent advances as proteinase, antigen and glycoprotein will be discussed. © 2009 Bentham Science Publishers Ltd. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09298673_v16_n24_p3174_Duschak http://hdl.handle.net/20.500.12110/paper_09298673_v16_n24_p3174_Duschak |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2,3,5,6 tetrafluorophenoxymethyl ketone alpha 2 macroglobulin antitrypanosomal agent aryl 4 oxothiazolylhydrazone derivative bisarylacylhydrazide derivative chagasin CpG oligodeoxynucleotide cruzipain cystatin cysteine proteinase cysteine proteinase inhibitor dipeptidyl allyl sulphone derivative dipeptidyl vinyl sulphone derivative hydrolase inhibitor k 11777 k 777 kininogen n methyl pip phenylalanine homo phenylalanine vinyl sulfonylurea phenyl peptide hydrolase inhibitor proteinase inhibitor recombinant cruzipain recombinant enzyme sialic acid unclassified drug virulence factor cruzipain cysteine proteinase cysteine proteinase inhibitor parasite antigen protozoal vaccine catalysis cell invasion Chagas disease chemical composition dog disease drug structure drug targeting enzyme analysis enzyme binding enzyme degradation enzyme mechanism enzyme purification enzyme structure enzyme substrate gene sequence glycosylation heart protection host parasite interaction immune response mammal nonhuman phagocyte protein analysis protein domain protein expression protein polymorphism protozoal infection review structure activity relation structure analysis target cell tissue injury Trypanosoma cruzi vaccine production animal Chagas disease chemistry enzymology human immunology metabolism mouse Trypanosoma cruzi Animals Antigens, Protozoan Chagas Disease Cysteine Endopeptidases Cysteine Proteinase Inhibitors Glycosylation Humans Mice Protozoan Vaccines Trypanosoma cruzi |
| spellingShingle |
2,3,5,6 tetrafluorophenoxymethyl ketone alpha 2 macroglobulin antitrypanosomal agent aryl 4 oxothiazolylhydrazone derivative bisarylacylhydrazide derivative chagasin CpG oligodeoxynucleotide cruzipain cystatin cysteine proteinase cysteine proteinase inhibitor dipeptidyl allyl sulphone derivative dipeptidyl vinyl sulphone derivative hydrolase inhibitor k 11777 k 777 kininogen n methyl pip phenylalanine homo phenylalanine vinyl sulfonylurea phenyl peptide hydrolase inhibitor proteinase inhibitor recombinant cruzipain recombinant enzyme sialic acid unclassified drug virulence factor cruzipain cysteine proteinase cysteine proteinase inhibitor parasite antigen protozoal vaccine catalysis cell invasion Chagas disease chemical composition dog disease drug structure drug targeting enzyme analysis enzyme binding enzyme degradation enzyme mechanism enzyme purification enzyme structure enzyme substrate gene sequence glycosylation heart protection host parasite interaction immune response mammal nonhuman phagocyte protein analysis protein domain protein expression protein polymorphism protozoal infection review structure activity relation structure analysis target cell tissue injury Trypanosoma cruzi vaccine production animal Chagas disease chemistry enzymology human immunology metabolism mouse Trypanosoma cruzi Animals Antigens, Protozoan Chagas Disease Cysteine Endopeptidases Cysteine Proteinase Inhibitors Glycosylation Humans Mice Protozoan Vaccines Trypanosoma cruzi Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review |
| topic_facet |
2,3,5,6 tetrafluorophenoxymethyl ketone alpha 2 macroglobulin antitrypanosomal agent aryl 4 oxothiazolylhydrazone derivative bisarylacylhydrazide derivative chagasin CpG oligodeoxynucleotide cruzipain cystatin cysteine proteinase cysteine proteinase inhibitor dipeptidyl allyl sulphone derivative dipeptidyl vinyl sulphone derivative hydrolase inhibitor k 11777 k 777 kininogen n methyl pip phenylalanine homo phenylalanine vinyl sulfonylurea phenyl peptide hydrolase inhibitor proteinase inhibitor recombinant cruzipain recombinant enzyme sialic acid unclassified drug virulence factor cruzipain cysteine proteinase cysteine proteinase inhibitor parasite antigen protozoal vaccine catalysis cell invasion Chagas disease chemical composition dog disease drug structure drug targeting enzyme analysis enzyme binding enzyme degradation enzyme mechanism enzyme purification enzyme structure enzyme substrate gene sequence glycosylation heart protection host parasite interaction immune response mammal nonhuman phagocyte protein analysis protein domain protein expression protein polymorphism protozoal infection review structure activity relation structure analysis target cell tissue injury Trypanosoma cruzi vaccine production animal Chagas disease chemistry enzymology human immunology metabolism mouse Trypanosoma cruzi Animals Antigens, Protozoan Chagas Disease Cysteine Endopeptidases Cysteine Proteinase Inhibitors Glycosylation Humans Mice Protozoan Vaccines Trypanosoma cruzi |
| description |
This review aims to present different aspects related to cruzipain, one of the most important proteins of the etiological agent of Chagas disease that has been extensively studied in the last two decades, including all the particularities of the molecule as well as to highlight its participation in multiple relevant functions of the parasite to favour the cell invasion phenomena, to facilitate host tissues proteolytic degradation and to trigger the evasion mechanism from host immune response. Cruzipain has been related with parasite metabolism and identified as both an important candidate for vaccine development and for trypanocidal drug design. We have reported for the first time that this enzyme is a sulfated glycoprotein. Indeed, the sulfated oligosaccharides are main targets for immune responses and are involved in tissue damage in mice immunized in absence of infection contributing to get deeper into the knowledge of the molecule composition and helping to elucidate its role in the infection and/or pathogenesis of the disease. A whole view including all the aspects related to the major cysteine proteinase of Trypanosoma cruzi studied so far including recent advances as proteinase, antigen and glycoprotein will be discussed. © 2009 Bentham Science Publishers Ltd. |
| title |
Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review |
| title_short |
Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review |
| title_full |
Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review |
| title_fullStr |
Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review |
| title_full_unstemmed |
Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review |
| title_sort |
cruzipain, the major cysteine protease of trypanosoma cruzi: a sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. a review |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09298673_v16_n24_p3174_Duschak http://hdl.handle.net/20.500.12110/paper_09298673_v16_n24_p3174_Duschak |
| _version_ |
1768543523000287232 |