Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina

Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum...

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Detalles Bibliográficos
Publicado: 2018
Materias:
EF1-α
Fusarium root rot
Fusarium wilt
Nicotiana tabacum
Pathogenicity
disease incidence
fungal disease
fungus
genetic analysis
molecular analysis
morphology
pathogenicity
phylogenetics
root rot
symptom
tobacco
virulence
Argentina
Fusarium
Fusarium oxysporum
Fusarium solani
Nicotiana obtusifolia
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09291873_v151_n4_p1065_Berruezo
http://hdl.handle.net/20.500.12110/paper_09291873_v151_n4_p1065_Berruezo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09291873_v151_n4_p1065_Berruezo
record_format dspace
spelling paper:paper_09291873_v151_n4_p1065_Berruezo2023-06-08T15:52:23Z Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina EF1-α Fusarium root rot Fusarium wilt Nicotiana tabacum Pathogenicity disease incidence fungal disease fungus genetic analysis molecular analysis morphology pathogenicity phylogenetics root rot symptom tobacco virulence Argentina Fusarium Fusarium oxysporum Fusarium solani Nicotiana obtusifolia Nicotiana tabacum Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production. © 2018, Koninklijke Nederlandse Planteziektenkundige Vereniging. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09291873_v151_n4_p1065_Berruezo http://hdl.handle.net/20.500.12110/paper_09291873_v151_n4_p1065_Berruezo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic EF1-α
Fusarium root rot
Fusarium wilt
Nicotiana tabacum
Pathogenicity
disease incidence
fungal disease
fungus
genetic analysis
molecular analysis
morphology
pathogenicity
phylogenetics
root rot
symptom
tobacco
virulence
Argentina
Fusarium
Fusarium oxysporum
Fusarium solani
Nicotiana obtusifolia
Nicotiana tabacum
spellingShingle EF1-α
Fusarium root rot
Fusarium wilt
Nicotiana tabacum
Pathogenicity
disease incidence
fungal disease
fungus
genetic analysis
molecular analysis
morphology
pathogenicity
phylogenetics
root rot
symptom
tobacco
virulence
Argentina
Fusarium
Fusarium oxysporum
Fusarium solani
Nicotiana obtusifolia
Nicotiana tabacum
Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
topic_facet EF1-α
Fusarium root rot
Fusarium wilt
Nicotiana tabacum
Pathogenicity
disease incidence
fungal disease
fungus
genetic analysis
molecular analysis
morphology
pathogenicity
phylogenetics
root rot
symptom
tobacco
virulence
Argentina
Fusarium
Fusarium oxysporum
Fusarium solani
Nicotiana obtusifolia
Nicotiana tabacum
description Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production. © 2018, Koninklijke Nederlandse Planteziektenkundige Vereniging.
title Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_short Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_full Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_fullStr Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_full_unstemmed Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
title_sort characterization of fusarium species associated with tobacco diseases in northwestern argentina
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09291873_v151_n4_p1065_Berruezo
http://hdl.handle.net/20.500.12110/paper_09291873_v151_n4_p1065_Berruezo
_version_ 1768545935574433792

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