Antibacterial activity of extracts and compounds isolated from the Andean medicinal plant Azorella cryptantha (Clos) Reiche, Apiaceae
Azorella cryptantha (Clos) Reiche, Apiaceae, vernacular name "yerba del soldado or cuerno de cabra" is a medicinal herb that grows in the Andean mountains (Argentina). An infusion or decoction of the leaves is employed as cholagogue and digestive, usually to treat food-borne illnesses asso...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09266690_v64_n_p152_Lima http://hdl.handle.net/20.500.12110/paper_09266690_v64_n_p152_Lima |
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paper:paper_09266690_v64_n_p152_Lima2023-06-08T15:51:39Z Antibacterial activity of extracts and compounds isolated from the Andean medicinal plant Azorella cryptantha (Clos) Reiche, Apiaceae Palermo, Jorge Alejandro Antibacterial activity Azorella cryptantha Chrysothol Madreporanone Stachytriol Bioassay Escherichia coli Ethers Microorganisms Nuclear magnetic resonance spectroscopy Plant extracts Plants (botany) Salmonella Anti-bacterial activity Azorella cryptantha Chrysothol Madreporanone Stachytriol Bacteria alcohol antimicrobial activity bacterium bioassay herb medicinal plant plant extract population structure Andenes Andoya Norway Vesteralen Apiaceae Azorella cryptantha Bacteria (microorganisms) Croton ovalifolius Enterobacteriaceae Escherichia coli Negibacteria Pseudomonas aeruginosa Salmonella enteritidis Salmonella sp. Staphylococcus aureus Yersinia Yersinia enterocolitica Azorella cryptantha (Clos) Reiche, Apiaceae, vernacular name "yerba del soldado or cuerno de cabra" is a medicinal herb that grows in the Andean mountains (Argentina). An infusion or decoction of the leaves is employed as cholagogue and digestive, usually to treat food-borne illnesses associated with enterobacteria. Extracts and compounds from two Argentinean populations were subjected to antibacterial assays against pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis and Yersinia enterocolitica) and Gram (+) Staphylococcus aureus methicillin-sensitive and methicillin-resistant microorganisms. The antibacterial activity-guided fractionation against a panel of ATCC and clinically isolated bacteria was done according to CLSI protocols. The petroleum ether extracts from both populations showed strong antibacterial activity against S. enteritidis with MIC values from 125 to 250μg/ml, and also towards methicillin-sensitive Staphylococcus aureus, and Gram negative strains E. coli, P. aeruginosa, Salmonella sp. and Yersinia enterocolítica-PI, (MICs between 400 and 1000μg/ml). Fractions from the petroleum extracts showed strong antimicrobial activity, against Escherichia coli ATCC 25922, E. coli-LM1, E. coli-LM2, and Salmonella enteritidis-MI, with MICs values between 31.2 and 125μg/ml. The bioassay-guided fractionation of the petroleum ether extracts led to the isolation of nine terpenes: azorellolide (1), mulinol (2), stachytriol (3),1α,10β,4β,5α-diepoxy-7β-germacran-6β-ol (4), 1β,10α,4β,5α-diepoxy-7β-germacran-6β-ol (5), 1,2,3,3α,4,5,6,7,8,8α-decahydro-7-(1-hydroxy-1-methylethyl)-1,4-dimethylazulene-3α,8α-diol (6), madreporanone (7), yaretol (8) and chrysotol or 6β,10β-epoxy-4α-hydroxyguaiane (9). Compounds 3, 5, 6 and 9 are reported here for the first time from A. cryptantha. Their structures and relative configurations have been determinated by means 1D and 2D NMR techniques. Chrysothol (9), madreporanone (7), and stachytriol (3) showed strong antimicrobial activities (MICs=50-100μg/ml) against enterobacteria E. coli and S. enteritidis. The antibacterial activity found for some of the isolated compounds supports least in part, the commercial exploited of this species to treat food-borne illnesses associated with Gram negative pathogenic bacteria. © 2014 Elsevier B.V. Fil:Palermo, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09266690_v64_n_p152_Lima http://hdl.handle.net/20.500.12110/paper_09266690_v64_n_p152_Lima |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antibacterial activity Azorella cryptantha Chrysothol Madreporanone Stachytriol Bioassay Escherichia coli Ethers Microorganisms Nuclear magnetic resonance spectroscopy Plant extracts Plants (botany) Salmonella Anti-bacterial activity Azorella cryptantha Chrysothol Madreporanone Stachytriol Bacteria alcohol antimicrobial activity bacterium bioassay herb medicinal plant plant extract population structure Andenes Andoya Norway Vesteralen Apiaceae Azorella cryptantha Bacteria (microorganisms) Croton ovalifolius Enterobacteriaceae Escherichia coli Negibacteria Pseudomonas aeruginosa Salmonella enteritidis Salmonella sp. Staphylococcus aureus Yersinia Yersinia enterocolitica |
| spellingShingle |
Antibacterial activity Azorella cryptantha Chrysothol Madreporanone Stachytriol Bioassay Escherichia coli Ethers Microorganisms Nuclear magnetic resonance spectroscopy Plant extracts Plants (botany) Salmonella Anti-bacterial activity Azorella cryptantha Chrysothol Madreporanone Stachytriol Bacteria alcohol antimicrobial activity bacterium bioassay herb medicinal plant plant extract population structure Andenes Andoya Norway Vesteralen Apiaceae Azorella cryptantha Bacteria (microorganisms) Croton ovalifolius Enterobacteriaceae Escherichia coli Negibacteria Pseudomonas aeruginosa Salmonella enteritidis Salmonella sp. Staphylococcus aureus Yersinia Yersinia enterocolitica Palermo, Jorge Alejandro Antibacterial activity of extracts and compounds isolated from the Andean medicinal plant Azorella cryptantha (Clos) Reiche, Apiaceae |
| topic_facet |
Antibacterial activity Azorella cryptantha Chrysothol Madreporanone Stachytriol Bioassay Escherichia coli Ethers Microorganisms Nuclear magnetic resonance spectroscopy Plant extracts Plants (botany) Salmonella Anti-bacterial activity Azorella cryptantha Chrysothol Madreporanone Stachytriol Bacteria alcohol antimicrobial activity bacterium bioassay herb medicinal plant plant extract population structure Andenes Andoya Norway Vesteralen Apiaceae Azorella cryptantha Bacteria (microorganisms) Croton ovalifolius Enterobacteriaceae Escherichia coli Negibacteria Pseudomonas aeruginosa Salmonella enteritidis Salmonella sp. Staphylococcus aureus Yersinia Yersinia enterocolitica |
| description |
Azorella cryptantha (Clos) Reiche, Apiaceae, vernacular name "yerba del soldado or cuerno de cabra" is a medicinal herb that grows in the Andean mountains (Argentina). An infusion or decoction of the leaves is employed as cholagogue and digestive, usually to treat food-borne illnesses associated with enterobacteria. Extracts and compounds from two Argentinean populations were subjected to antibacterial assays against pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis and Yersinia enterocolitica) and Gram (+) Staphylococcus aureus methicillin-sensitive and methicillin-resistant microorganisms. The antibacterial activity-guided fractionation against a panel of ATCC and clinically isolated bacteria was done according to CLSI protocols. The petroleum ether extracts from both populations showed strong antibacterial activity against S. enteritidis with MIC values from 125 to 250μg/ml, and also towards methicillin-sensitive Staphylococcus aureus, and Gram negative strains E. coli, P. aeruginosa, Salmonella sp. and Yersinia enterocolítica-PI, (MICs between 400 and 1000μg/ml). Fractions from the petroleum extracts showed strong antimicrobial activity, against Escherichia coli ATCC 25922, E. coli-LM1, E. coli-LM2, and Salmonella enteritidis-MI, with MICs values between 31.2 and 125μg/ml. The bioassay-guided fractionation of the petroleum ether extracts led to the isolation of nine terpenes: azorellolide (1), mulinol (2), stachytriol (3),1α,10β,4β,5α-diepoxy-7β-germacran-6β-ol (4), 1β,10α,4β,5α-diepoxy-7β-germacran-6β-ol (5), 1,2,3,3α,4,5,6,7,8,8α-decahydro-7-(1-hydroxy-1-methylethyl)-1,4-dimethylazulene-3α,8α-diol (6), madreporanone (7), yaretol (8) and chrysotol or 6β,10β-epoxy-4α-hydroxyguaiane (9). Compounds 3, 5, 6 and 9 are reported here for the first time from A. cryptantha. Their structures and relative configurations have been determinated by means 1D and 2D NMR techniques. Chrysothol (9), madreporanone (7), and stachytriol (3) showed strong antimicrobial activities (MICs=50-100μg/ml) against enterobacteria E. coli and S. enteritidis. The antibacterial activity found for some of the isolated compounds supports least in part, the commercial exploited of this species to treat food-borne illnesses associated with Gram negative pathogenic bacteria. © 2014 Elsevier B.V. |
| author |
Palermo, Jorge Alejandro |
| author_facet |
Palermo, Jorge Alejandro |
| author_sort |
Palermo, Jorge Alejandro |
| title |
Antibacterial activity of extracts and compounds isolated from the Andean medicinal plant Azorella cryptantha (Clos) Reiche, Apiaceae |
| title_short |
Antibacterial activity of extracts and compounds isolated from the Andean medicinal plant Azorella cryptantha (Clos) Reiche, Apiaceae |
| title_full |
Antibacterial activity of extracts and compounds isolated from the Andean medicinal plant Azorella cryptantha (Clos) Reiche, Apiaceae |
| title_fullStr |
Antibacterial activity of extracts and compounds isolated from the Andean medicinal plant Azorella cryptantha (Clos) Reiche, Apiaceae |
| title_full_unstemmed |
Antibacterial activity of extracts and compounds isolated from the Andean medicinal plant Azorella cryptantha (Clos) Reiche, Apiaceae |
| title_sort |
antibacterial activity of extracts and compounds isolated from the andean medicinal plant azorella cryptantha (clos) reiche, apiaceae |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09266690_v64_n_p152_Lima http://hdl.handle.net/20.500.12110/paper_09266690_v64_n_p152_Lima |
| work_keys_str_mv |
AT palermojorgealejandro antibacterialactivityofextractsandcompoundsisolatedfromtheandeanmedicinalplantazorellacryptanthaclosreicheapiaceae |
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1768541995618271232 |