Genome and rye chromosome distribution in tricepiro "Don René INTA", a synthetic forage

The tricepiro "Don René INTA" is an artificial hybrid with 2n = 42 chromosomes, including 14 rye (RR) and 28 wheat (AABB) chromosomes, with introgression of Thinopyrum ponticum (Podp.) Barkw. et Dewey on chromosome 6A. The aim of this work was to study the spatial distribution of the genom...

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Detalles Bibliográficos
Autores principales: Fradkin, Maia, Ferrari, María Rosa, Remis, Maria Isabel, Greizerstein, Eduardo José, Poggio, Lidia
Publicado: 2012
Materias:
Fluorescence in situ hybridization
Genome in situ hybridization
Genomic mean distance
pSc119.2
Thinopyrum ponticum
Tricepiro
Triticosecale
chromosome
cytogenetics
cytology
fluorescence
forage
genetic analysis
genetic engineering
genome
grass
hybridization
introgression
spatial distribution
wheat
Secale cereale
Triticum aestivum
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09259864_v59_n7_p1281_Fradkin
http://hdl.handle.net/20.500.12110/paper_09259864_v59_n7_p1281_Fradkin
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09259864_v59_n7_p1281_Fradkin
record_format dspace
spelling paper:paper_09259864_v59_n7_p1281_Fradkin2023-06-08T15:51:32Z Genome and rye chromosome distribution in tricepiro "Don René INTA", a synthetic forage Fradkin, Maia Ferrari, María Rosa Remis, Maria Isabel Greizerstein, Eduardo José Poggio, Lidia Fluorescence in situ hybridization Genome in situ hybridization Genomic mean distance pSc119.2 Thinopyrum ponticum Tricepiro Triticosecale chromosome cytogenetics cytology fluorescence forage genetic analysis genetic engineering genome grass hybridization introgression spatial distribution wheat Secale cereale Thinopyrum ponticum Triticosecale Triticum aestivum The tricepiro "Don René INTA" is an artificial hybrid with 2n = 42 chromosomes, including 14 rye (RR) and 28 wheat (AABB) chromosomes, with introgression of Thinopyrum ponticum (Podp.) Barkw. et Dewey on chromosome 6A. The aim of this work was to study the spatial distribution of the genomes and chromosomes of rye and wheat in metaphase cells from the root tip of this hybrid. The rye chromosomes were recognized by genome in situ hybridization using total genomic DNA as a probe of rye and wheat DNA as the blocking agent. Two points were determined in each cell: one representing the genomic mean distance of rye chromosomes (G RMD) and the other the genomic mean distance of wheat chromosomes (G WMD). The distance between the rye chromosomes and G RMD and G WMD showed no statistically significant difference, thus indicating that there would be no differential spatial domains for both genomes. The fluorescence in situ hybridization analysis of rye chromosomes using the pSc119. 2 probe suggested that all chromosome pairs present somatic association in mitotic metaphase. © 2012 Springer Science+Business Media Dordrecht. Fil:Fradkin, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferrari, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Remis, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Greizerstein, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09259864_v59_n7_p1281_Fradkin http://hdl.handle.net/20.500.12110/paper_09259864_v59_n7_p1281_Fradkin
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Fluorescence in situ hybridization
Genome in situ hybridization
Genomic mean distance
pSc119.2
Thinopyrum ponticum
Tricepiro
Triticosecale
chromosome
cytogenetics
cytology
fluorescence
forage
genetic analysis
genetic engineering
genome
grass
hybridization
introgression
spatial distribution
wheat
Secale cereale
Thinopyrum ponticum
Triticosecale
Triticum aestivum
spellingShingle Fluorescence in situ hybridization
Genome in situ hybridization
Genomic mean distance
pSc119.2
Thinopyrum ponticum
Tricepiro
Triticosecale
chromosome
cytogenetics
cytology
fluorescence
forage
genetic analysis
genetic engineering
genome
grass
hybridization
introgression
spatial distribution
wheat
Secale cereale
Thinopyrum ponticum
Triticosecale
Triticum aestivum
Fradkin, Maia
Ferrari, María Rosa
Remis, Maria Isabel
Greizerstein, Eduardo José
Poggio, Lidia
Genome and rye chromosome distribution in tricepiro "Don René INTA", a synthetic forage
topic_facet Fluorescence in situ hybridization
Genome in situ hybridization
Genomic mean distance
pSc119.2
Thinopyrum ponticum
Tricepiro
Triticosecale
chromosome
cytogenetics
cytology
fluorescence
forage
genetic analysis
genetic engineering
genome
grass
hybridization
introgression
spatial distribution
wheat
Secale cereale
Thinopyrum ponticum
Triticosecale
Triticum aestivum
description The tricepiro "Don René INTA" is an artificial hybrid with 2n = 42 chromosomes, including 14 rye (RR) and 28 wheat (AABB) chromosomes, with introgression of Thinopyrum ponticum (Podp.) Barkw. et Dewey on chromosome 6A. The aim of this work was to study the spatial distribution of the genomes and chromosomes of rye and wheat in metaphase cells from the root tip of this hybrid. The rye chromosomes were recognized by genome in situ hybridization using total genomic DNA as a probe of rye and wheat DNA as the blocking agent. Two points were determined in each cell: one representing the genomic mean distance of rye chromosomes (G RMD) and the other the genomic mean distance of wheat chromosomes (G WMD). The distance between the rye chromosomes and G RMD and G WMD showed no statistically significant difference, thus indicating that there would be no differential spatial domains for both genomes. The fluorescence in situ hybridization analysis of rye chromosomes using the pSc119. 2 probe suggested that all chromosome pairs present somatic association in mitotic metaphase. © 2012 Springer Science+Business Media Dordrecht.
author Fradkin, Maia
Ferrari, María Rosa
Remis, Maria Isabel
Greizerstein, Eduardo José
Poggio, Lidia
author_facet Fradkin, Maia
Ferrari, María Rosa
Remis, Maria Isabel
Greizerstein, Eduardo José
Poggio, Lidia
author_sort Fradkin, Maia
title Genome and rye chromosome distribution in tricepiro "Don René INTA", a synthetic forage
title_short Genome and rye chromosome distribution in tricepiro "Don René INTA", a synthetic forage
title_full Genome and rye chromosome distribution in tricepiro "Don René INTA", a synthetic forage
title_fullStr Genome and rye chromosome distribution in tricepiro "Don René INTA", a synthetic forage
title_full_unstemmed Genome and rye chromosome distribution in tricepiro "Don René INTA", a synthetic forage
title_sort genome and rye chromosome distribution in tricepiro "don rené inta", a synthetic forage
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09259864_v59_n7_p1281_Fradkin
http://hdl.handle.net/20.500.12110/paper_09259864_v59_n7_p1281_Fradkin
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