The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis
We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin+ primary neurons and modulated Gli3 and Zic2 according to their roles i...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09254773_v107_n1-2_p119_Paganelli http://hdl.handle.net/20.500.12110/paper_09254773_v107_n1-2_p119_Paganelli |
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paper:paper_09254773_v107_n1-2_p119_Paganelli2023-06-08T15:51:21Z The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis Apoptosis Morpholino oligonucleotide Presenilin Primary neurogenesis Proliferation Sonic hedgehog Xenopus laevis antisense oligonucleotide messenger RNA presenilin 1 sonic hedgehog protein Alzheimer disease apoptosis article controlled study embryo gene expression regulation immunohistochemistry nervous system development nonhuman priority journal reverse transcription polymerase chain reaction Xenopus Xenopus We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin+ primary neurons and modulated Gli3 and Zic2 according to their roles in activating and repressing primary neurogenesis, respectively. Presenilin increased shh expression within its normal domain, mainly in the floor plate, whereas an antisense X-presenilin-α morpholino oligonucleotide reduced shh expression. Both shh and presenilin promoted cell proliferation and apoptosis, but the effects of shh were widely distributed, while those resulting from presenilin injection coincided with the range of shh signaling. We suggest that presenilin may modulate primary neurogenesis, proliferation, and apoptosis in the neural plate, through the enhancement of shh signaling. Copyright © 2001 Elsevier Science Ireland Ltd. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09254773_v107_n1-2_p119_Paganelli http://hdl.handle.net/20.500.12110/paper_09254773_v107_n1-2_p119_Paganelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Morpholino oligonucleotide Presenilin Primary neurogenesis Proliferation Sonic hedgehog Xenopus laevis antisense oligonucleotide messenger RNA presenilin 1 sonic hedgehog protein Alzheimer disease apoptosis article controlled study embryo gene expression regulation immunohistochemistry nervous system development nonhuman priority journal reverse transcription polymerase chain reaction Xenopus Xenopus |
| spellingShingle |
Apoptosis Morpholino oligonucleotide Presenilin Primary neurogenesis Proliferation Sonic hedgehog Xenopus laevis antisense oligonucleotide messenger RNA presenilin 1 sonic hedgehog protein Alzheimer disease apoptosis article controlled study embryo gene expression regulation immunohistochemistry nervous system development nonhuman priority journal reverse transcription polymerase chain reaction Xenopus Xenopus The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| topic_facet |
Apoptosis Morpholino oligonucleotide Presenilin Primary neurogenesis Proliferation Sonic hedgehog Xenopus laevis antisense oligonucleotide messenger RNA presenilin 1 sonic hedgehog protein Alzheimer disease apoptosis article controlled study embryo gene expression regulation immunohistochemistry nervous system development nonhuman priority journal reverse transcription polymerase chain reaction Xenopus Xenopus |
| description |
We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin+ primary neurons and modulated Gli3 and Zic2 according to their roles in activating and repressing primary neurogenesis, respectively. Presenilin increased shh expression within its normal domain, mainly in the floor plate, whereas an antisense X-presenilin-α morpholino oligonucleotide reduced shh expression. Both shh and presenilin promoted cell proliferation and apoptosis, but the effects of shh were widely distributed, while those resulting from presenilin injection coincided with the range of shh signaling. We suggest that presenilin may modulate primary neurogenesis, proliferation, and apoptosis in the neural plate, through the enhancement of shh signaling. Copyright © 2001 Elsevier Science Ireland Ltd. |
| title |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_short |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_full |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_fullStr |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_full_unstemmed |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_sort |
alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in xenopus primary neurogenesis |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09254773_v107_n1-2_p119_Paganelli http://hdl.handle.net/20.500.12110/paper_09254773_v107_n1-2_p119_Paganelli |
| _version_ |
1768545380759240704 |