Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir
The anti-HSV-1 activity of meliacine (MA), a peptide isolated from leaves of Melia azedarach L., alone and in combination with acyclovir (ACV), was assayed against thymidine kinase-deficient (TK-) virus yields in vitro. MA alone proved to inhibit significantly TK- viral replication, whereas ACV was...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09248579_v9_n1_p49_Barquero http://hdl.handle.net/20.500.12110/paper_09248579_v9_n1_p49_Barquero |
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paper:paper_09248579_v9_n1_p49_Barquero2025-07-30T18:27:24Z Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir Acyclovir Drug combination Herpes simplex virus type 12 Meliacine aciclovir antivirus agent melia azedarach extract meliacine peptide plant extract thymidine kinase unclassified drug animal cell antigen expression antiviral activity article cell viability concentration response controlled study cytotoxicity drug isolation drug potentiation enzyme deficiency herpes simplex virus 1 immunofluorescence test nonhuman plant leaf priority journal vero cell virus mutant virus replication The anti-HSV-1 activity of meliacine (MA), a peptide isolated from leaves of Melia azedarach L., alone and in combination with acyclovir (ACV), was assayed against thymidine kinase-deficient (TK-) virus yields in vitro. MA alone proved to inhibit significantly TK- viral replication, whereas ACV was more potent than MA as an inhibitor of TK+ replication. TK- and TK+ synergistic inhibition by the combination of both agents was observed at concentrations that did not alter cell viability. The interaction between MA and ACV was quantitatively determined by calculating the combination index and plotting the data by the isobologram method. Besides, MA and ACV were able to suppress synergistically the antigen expression on HSV-1 infected cells processed by an immunofluorescence assay. These in vitro findings suggest that combinations of MA and ACV at appropriate doses may provide an increased efficacy in inhibiting both TK- and TK+ HSV-1 multiplication. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09248579_v9_n1_p49_Barquero http://hdl.handle.net/20.500.12110/paper_09248579_v9_n1_p49_Barquero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acyclovir Drug combination Herpes simplex virus type 12 Meliacine aciclovir antivirus agent melia azedarach extract meliacine peptide plant extract thymidine kinase unclassified drug animal cell antigen expression antiviral activity article cell viability concentration response controlled study cytotoxicity drug isolation drug potentiation enzyme deficiency herpes simplex virus 1 immunofluorescence test nonhuman plant leaf priority journal vero cell virus mutant virus replication |
| spellingShingle |
Acyclovir Drug combination Herpes simplex virus type 12 Meliacine aciclovir antivirus agent melia azedarach extract meliacine peptide plant extract thymidine kinase unclassified drug animal cell antigen expression antiviral activity article cell viability concentration response controlled study cytotoxicity drug isolation drug potentiation enzyme deficiency herpes simplex virus 1 immunofluorescence test nonhuman plant leaf priority journal vero cell virus mutant virus replication Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir |
| topic_facet |
Acyclovir Drug combination Herpes simplex virus type 12 Meliacine aciclovir antivirus agent melia azedarach extract meliacine peptide plant extract thymidine kinase unclassified drug animal cell antigen expression antiviral activity article cell viability concentration response controlled study cytotoxicity drug isolation drug potentiation enzyme deficiency herpes simplex virus 1 immunofluorescence test nonhuman plant leaf priority journal vero cell virus mutant virus replication |
| description |
The anti-HSV-1 activity of meliacine (MA), a peptide isolated from leaves of Melia azedarach L., alone and in combination with acyclovir (ACV), was assayed against thymidine kinase-deficient (TK-) virus yields in vitro. MA alone proved to inhibit significantly TK- viral replication, whereas ACV was more potent than MA as an inhibitor of TK+ replication. TK- and TK+ synergistic inhibition by the combination of both agents was observed at concentrations that did not alter cell viability. The interaction between MA and ACV was quantitatively determined by calculating the combination index and plotting the data by the isobologram method. Besides, MA and ACV were able to suppress synergistically the antigen expression on HSV-1 infected cells processed by an immunofluorescence assay. These in vitro findings suggest that combinations of MA and ACV at appropriate doses may provide an increased efficacy in inhibiting both TK- and TK+ HSV-1 multiplication. |
| title |
Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir |
| title_short |
Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir |
| title_full |
Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir |
| title_fullStr |
Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir |
| title_full_unstemmed |
Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir |
| title_sort |
antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of herpes simplex virus type 1 alone and in combination with acyclovir |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09248579_v9_n1_p49_Barquero http://hdl.handle.net/20.500.12110/paper_09248579_v9_n1_p49_Barquero |
| _version_ |
1840321569513013248 |