Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli
UV treatment increases the frequency of Tn10 precise excision from different sites of the Escherichia coli chromosome. UV induction of Tn10 excision is not evidenced in a lexA3 (ind-) mutant carrying either a recA+ or a recA730 allele. High levels of RecA synthesized by a recA+ gene not repressible...
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1993
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09218777_v293_n3_p241_Levy http://hdl.handle.net/20.500.12110/paper_09218777_v293_n3_p241_Levy |
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paper:paper_09218777_v293_n3_p241_Levy2023-06-08T15:50:51Z Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli Levy, Myriam Susana Nagel de Zwaig, Rosa Deletions lexA, umuC, recA mutations pKM101 Tn10 precise excision UV induction article dna repair escherichia coli excision repair mutagenesis mutant mutation nonhuman priority journal ultraviolet radiation Bacterial Proteins DNA Transposable Elements Escherichia coli Genes, Bacterial Mutation Plasmids Rec A Protein SOS Response (Genetics) Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Ultraviolet Rays Escherichia coli UV treatment increases the frequency of Tn10 precise excision from different sites of the Escherichia coli chromosome. UV induction of Tn10 excision is not evidenced in a lexA3 (ind-) mutant carrying either a recA+ or a recA730 allele. High levels of RecA synthesized by a recA+ gene not repressible by LexA do not relieve the non-inducibility of Tn10 excision in a lexA3 (ind-) background. This indicates that the expression of an SOS gene different from recA is necessary for the induction of Tn10 excision. In contrast to UV induction of point mutations, this induction does not depend on a functional umuC gene since umuC::Tn5 mutants show increased levels of Tn10 excision from leu, thr or gal after irradiation. MucAB+ plasmid pKM101 which renders cells more UV-mutable for point mutations decreases UV-induced Tn10 excision. These results show that UV-induced Tn10 precise excision requires SOS induction and that it involves a pathway different from point mutagenesis. © 1993. Fil:Levy, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nagel, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09218777_v293_n3_p241_Levy http://hdl.handle.net/20.500.12110/paper_09218777_v293_n3_p241_Levy |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Deletions lexA, umuC, recA mutations pKM101 Tn10 precise excision UV induction article dna repair escherichia coli excision repair mutagenesis mutant mutation nonhuman priority journal ultraviolet radiation Bacterial Proteins DNA Transposable Elements Escherichia coli Genes, Bacterial Mutation Plasmids Rec A Protein SOS Response (Genetics) Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Ultraviolet Rays Escherichia coli |
| spellingShingle |
Deletions lexA, umuC, recA mutations pKM101 Tn10 precise excision UV induction article dna repair escherichia coli excision repair mutagenesis mutant mutation nonhuman priority journal ultraviolet radiation Bacterial Proteins DNA Transposable Elements Escherichia coli Genes, Bacterial Mutation Plasmids Rec A Protein SOS Response (Genetics) Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Ultraviolet Rays Escherichia coli Levy, Myriam Susana Nagel de Zwaig, Rosa Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli |
| topic_facet |
Deletions lexA, umuC, recA mutations pKM101 Tn10 precise excision UV induction article dna repair escherichia coli excision repair mutagenesis mutant mutation nonhuman priority journal ultraviolet radiation Bacterial Proteins DNA Transposable Elements Escherichia coli Genes, Bacterial Mutation Plasmids Rec A Protein SOS Response (Genetics) Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Ultraviolet Rays Escherichia coli |
| description |
UV treatment increases the frequency of Tn10 precise excision from different sites of the Escherichia coli chromosome. UV induction of Tn10 excision is not evidenced in a lexA3 (ind-) mutant carrying either a recA+ or a recA730 allele. High levels of RecA synthesized by a recA+ gene not repressible by LexA do not relieve the non-inducibility of Tn10 excision in a lexA3 (ind-) background. This indicates that the expression of an SOS gene different from recA is necessary for the induction of Tn10 excision. In contrast to UV induction of point mutations, this induction does not depend on a functional umuC gene since umuC::Tn5 mutants show increased levels of Tn10 excision from leu, thr or gal after irradiation. MucAB+ plasmid pKM101 which renders cells more UV-mutable for point mutations decreases UV-induced Tn10 excision. These results show that UV-induced Tn10 precise excision requires SOS induction and that it involves a pathway different from point mutagenesis. © 1993. |
| author |
Levy, Myriam Susana Nagel de Zwaig, Rosa |
| author_facet |
Levy, Myriam Susana Nagel de Zwaig, Rosa |
| author_sort |
Levy, Myriam Susana |
| title |
Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli |
| title_short |
Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli |
| title_full |
Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli |
| title_fullStr |
Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli |
| title_full_unstemmed |
Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli |
| title_sort |
effect of mutations in sos genes on uv-induced precise excision of tn10 in escherichia coli |
| publishDate |
1993 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09218777_v293_n3_p241_Levy http://hdl.handle.net/20.500.12110/paper_09218777_v293_n3_p241_Levy |
| work_keys_str_mv |
AT levymyriamsusana effectofmutationsinsosgenesonuvinducedpreciseexcisionoftn10inescherichiacoli AT nageldezwaigrosa effectofmutationsinsosgenesonuvinducedpreciseexcisionoftn10inescherichiacoli |
| _version_ |
1768542558037737472 |