Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications,...
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paper:paper_09208569_v38_n3_p455_Distefano2023-06-08T15:50:15Z Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Escherichia coli Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Escherichia coli Fijivirus Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Reoviridae Zea mays Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein. © 2009 Springer Science+Business Media, LLC. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09208569_v38_n3_p455_Distefano http://hdl.handle.net/20.500.12110/paper_09208569_v38_n3_p455_Distefano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Escherichia coli Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Escherichia coli Fijivirus Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Reoviridae Zea mays |
| spellingShingle |
Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Escherichia coli Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Escherichia coli Fijivirus Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Reoviridae Zea mays Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| topic_facet |
Core protein Fijivirus Maize MRCV Structural virus proteins capsid protein polyclonal antiserum structural protein animal experiment article controlled study Escherichia coli female immunization immunoelectron microscopy immunogold labeling mal de rio cuarto virus nonhuman priority journal protein expression protein structure rabbit Reovirus virus genome virus particle virus purification Antibodies, Viral Capsid Proteins Escherichia coli Microscopy, Immunoelectron Recombinant Fusion Proteins Reoviridae Virion Escherichia coli Fijivirus Mal de Rio Cuarto virus Miridae Oryctolagus cuniculus Reoviridae Zea mays |
| description |
Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein. © 2009 Springer Science+Business Media, LLC. |
| author |
Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana |
| author_facet |
Distéfano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana |
| author_sort |
Distéfano, Ana Julia |
| title |
Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_short |
Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_full |
Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_fullStr |
Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_full_unstemmed |
Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein |
| title_sort |
mal de río cuarto virus (mrcv) genomic segment s3 codes for the major core capsid protein |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09208569_v38_n3_p455_Distefano http://hdl.handle.net/20.500.12110/paper_09208569_v38_n3_p455_Distefano |
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AT distefanoanajulia malderiocuartovirusmrcvgenomicsegments3codesforthemajorcorecapsidprotein AT hopphoracioesteban malderiocuartovirusmrcvgenomicsegments3codesforthemajorcorecapsidprotein AT delvasmariana malderiocuartovirusmrcvgenomicsegments3codesforthemajorcorecapsidprotein |
| _version_ |
1768543428749033472 |