Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)

This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain of the protogynous swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM), on final matu...

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Detalles Bibliográficos
Publicado: 1997
Materias:
Diandric
Domperidone
Fish
GnRH
Hermaphroditism
Protogynous
Sex reversal
Spermiation
Synbranchidae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v16_n5_p425_Ravaglia
http://hdl.handle.net/20.500.12110/paper_09201742_v16_n5_p425_Ravaglia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09201742_v16_n5_p425_Ravaglia
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spelling paper:paper_09201742_v16_n5_p425_Ravaglia2023-06-08T15:50:03Z Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae) Diandric Domperidone Fish GnRH Hermaphroditism Protogynous Sex reversal Spermiation Synbranchidae This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain of the protogynous swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM), on final maturation and gamete release in this species. Evidence for the presence of two GnRH variants, sGnRH and cIIGnRH were obtained by reverse phase high-pressure liquid chromatography (RP-HPLC) and radioimmunoassay with different antisera. The effects of treatment with sGnRH-A+DOM were checked by three ways: oocyte and milt release by stripping, histological analysis of the gonadal tissue, and androgen serum levels at different times throughout the experiment. In males, spermiation was induced after three weeks of treatment. In the female group, sGnRH-A+DOM did not induce ovulation at the end of the experiment. Histological analysis of the gonads from the female group showed evidence of sex reversal. All the treated fish had elevated androgen serum levels from the third week, with respect to control fish. In all cases, serum estradiol levels were undetectable. These results suggest that treatment with sGnRH analog and DOM induce sex reversal in female and spermiation in males. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v16_n5_p425_Ravaglia http://hdl.handle.net/20.500.12110/paper_09201742_v16_n5_p425_Ravaglia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Diandric
Domperidone
Fish
GnRH
Hermaphroditism
Protogynous
Sex reversal
Spermiation
Synbranchidae
spellingShingle Diandric
Domperidone
Fish
GnRH
Hermaphroditism
Protogynous
Sex reversal
Spermiation
Synbranchidae
Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
topic_facet Diandric
Domperidone
Fish
GnRH
Hermaphroditism
Protogynous
Sex reversal
Spermiation
Synbranchidae
description This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain of the protogynous swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM), on final maturation and gamete release in this species. Evidence for the presence of two GnRH variants, sGnRH and cIIGnRH were obtained by reverse phase high-pressure liquid chromatography (RP-HPLC) and radioimmunoassay with different antisera. The effects of treatment with sGnRH-A+DOM were checked by three ways: oocyte and milt release by stripping, histological analysis of the gonadal tissue, and androgen serum levels at different times throughout the experiment. In males, spermiation was induced after three weeks of treatment. In the female group, sGnRH-A+DOM did not induce ovulation at the end of the experiment. Histological analysis of the gonads from the female group showed evidence of sex reversal. All the treated fish had elevated androgen serum levels from the third week, with respect to control fish. In all cases, serum estradiol levels were undetectable. These results suggest that treatment with sGnRH analog and DOM induce sex reversal in female and spermiation in males.
title Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_short Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_full Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_fullStr Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_full_unstemmed Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)
title_sort characterization of molecular variants of gnrh, induction of spermiation and sex reversal using salmon gnrh-a and domperidone in the protogynous diandric fish, synbranchus marmoratus bloch, (teleostei, synbranchidae)
publishDate 1997
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v16_n5_p425_Ravaglia
http://hdl.handle.net/20.500.12110/paper_09201742_v16_n5_p425_Ravaglia
_version_ 1768541803545362432

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