Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress

The encapsulation of living cells in materials with good optical and mechanical properties often produces death or stress due to the release of toxic byproducts originated during the synthesis. We present here a method to assess the cellular stress that silica entrapment exerts over living cells tak...

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Detalles Bibliográficos
Publicado:	2008
Materias:	Cell growth Functional groups Mechanical properties Optimization Toxic materials Cellular stress Living cells Optimizing silica encapsulation Toxic byproducts Silica
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08974756_v20_n9_p3015_Perullini http://hdl.handle.net/20.500.12110/paper_08974756_v20_n9_p3015_Perullini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_08974756_v20_n9_p3015_Perullini
record_format	dspace
spelling	paper:paper_08974756_v20_n9_p3015_Perullini2025-07-30T18:25:36Z Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress Cell growth Functional groups Mechanical properties Optimization Toxic materials Cellular stress Living cells Optimizing silica encapsulation Toxic byproducts Silica The encapsulation of living cells in materials with good optical and mechanical properties often produces death or stress due to the release of toxic byproducts originated during the synthesis. We present here a method to assess the cellular stress that silica entrapment exerts over living cells taking into account the main preparation variables such as the nature of the silica source, protecting functional groups, total solid concentration, or indirect procedures. Measurement of the cellular stress status of genetically modified Sacharomyces cerevisiae, a true biological probe, allowed us to perform a quantitative analysis of cellular stress in a short time basis (compared to conventional long-term viability tests), opening the gate for a more sophisticated approach to optimize the synthesis conditions. In addition, the aforementioned findings allowed the preparation of novel materials with enhanced optical and mechanical properties. The relation of cellular stress with initial viability is also discussed. © 2008 American Chemical Society. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08974756_v20_n9_p3015_Perullini http://hdl.handle.net/20.500.12110/paper_08974756_v20_n9_p3015_Perullini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell growth Functional groups Mechanical properties Optimization Toxic materials Cellular stress Living cells Optimizing silica encapsulation Toxic byproducts Silica
spellingShingle	Cell growth Functional groups Mechanical properties Optimization Toxic materials Cellular stress Living cells Optimizing silica encapsulation Toxic byproducts Silica Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress
topic_facet	Cell growth Functional groups Mechanical properties Optimization Toxic materials Cellular stress Living cells Optimizing silica encapsulation Toxic byproducts Silica
description	The encapsulation of living cells in materials with good optical and mechanical properties often produces death or stress due to the release of toxic byproducts originated during the synthesis. We present here a method to assess the cellular stress that silica entrapment exerts over living cells taking into account the main preparation variables such as the nature of the silica source, protecting functional groups, total solid concentration, or indirect procedures. Measurement of the cellular stress status of genetically modified Sacharomyces cerevisiae, a true biological probe, allowed us to perform a quantitative analysis of cellular stress in a short time basis (compared to conventional long-term viability tests), opening the gate for a more sophisticated approach to optimize the synthesis conditions. In addition, the aforementioned findings allowed the preparation of novel materials with enhanced optical and mechanical properties. The relation of cellular stress with initial viability is also discussed. © 2008 American Chemical Society.
title	Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress
title_short	Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress
title_full	Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress
title_fullStr	Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress
title_full_unstemmed	Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress
title_sort	optimizing silica encapsulation of living cells: in situ evaluation of cellular stress
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08974756_v20_n9_p3015_Perullini http://hdl.handle.net/20.500.12110/paper_08974756_v20_n9_p3015_Perullini
_version_	1840324739341484032

Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress

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