Characterization of two casein kinase activities in the fungus Mucor rouxii
Two cyclic-nucleotide independent soluble casein kinase activities (CK I and CK II) from the fungus Mucor rouxii have been isolated, characterized and found to fit in the general classification of type 1 (CK I) and 2 (CK II) casein kinases, according to their enzymatic and structural properties. Bot...
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1988
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08957479_v12_n4_p183_Pardo http://hdl.handle.net/20.500.12110/paper_08957479_v12_n4_p183_Pardo |
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paper:paper_08957479_v12_n4_p183_Pardo2023-06-08T15:48:05Z Characterization of two casein kinase activities in the fungus Mucor rouxii Pardo, Patricia Susana Moreno, Silvia casein kinase radioisotope serine threonine fungus mucor rouxii nonhuman priority journal Amino Acids Centrifugation, Density Gradient Chromatography, Affinity Chromatography, Gel Comparative Study Heparin Kinetics Mucor Phosphorylation Polyamines Protein Kinases Solubility Substrate Specificity Support, Non-U.S. Gov't Two cyclic-nucleotide independent soluble casein kinase activities (CK I and CK II) from the fungus Mucor rouxii have been isolated, characterized and found to fit in the general classification of type 1 (CK I) and 2 (CK II) casein kinases, according to their enzymatic and structural properties. Both enzymes phosphorylate acidic substrates, require Mg2+ and have a chromatographic behaviour on DEAE-Sepharose and phosphocellulose similar to their mammalian counterparts. CK I has a sedimentation coefficient of 3.5 S, uses ATP as a phosphate donor (K(m) = 40 μM), phosphorylates casein mainly on serine residues, its activity is strongly inhibited by KCl and polyamines. CK II has a sedimentation coefficient of 7.4 S, uses ATP and GTP as phosphate donors (K(m) ATP = 10 μM; K(m) GTP = 40 μM), phosphorylates casein in serine and threonine, its activity is stimulated by KCl and by polyamines and is inhibited by heparin (I50 = 0.5 μg/ml). Casein kinase activity associated to particulate fraction (40% of total) has been partially characterized and shown to be similar to the soluble CK I activity. Fil:Pardo, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08957479_v12_n4_p183_Pardo http://hdl.handle.net/20.500.12110/paper_08957479_v12_n4_p183_Pardo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
casein kinase radioisotope serine threonine fungus mucor rouxii nonhuman priority journal Amino Acids Centrifugation, Density Gradient Chromatography, Affinity Chromatography, Gel Comparative Study Heparin Kinetics Mucor Phosphorylation Polyamines Protein Kinases Solubility Substrate Specificity Support, Non-U.S. Gov't |
| spellingShingle |
casein kinase radioisotope serine threonine fungus mucor rouxii nonhuman priority journal Amino Acids Centrifugation, Density Gradient Chromatography, Affinity Chromatography, Gel Comparative Study Heparin Kinetics Mucor Phosphorylation Polyamines Protein Kinases Solubility Substrate Specificity Support, Non-U.S. Gov't Pardo, Patricia Susana Moreno, Silvia Characterization of two casein kinase activities in the fungus Mucor rouxii |
| topic_facet |
casein kinase radioisotope serine threonine fungus mucor rouxii nonhuman priority journal Amino Acids Centrifugation, Density Gradient Chromatography, Affinity Chromatography, Gel Comparative Study Heparin Kinetics Mucor Phosphorylation Polyamines Protein Kinases Solubility Substrate Specificity Support, Non-U.S. Gov't |
| description |
Two cyclic-nucleotide independent soluble casein kinase activities (CK I and CK II) from the fungus Mucor rouxii have been isolated, characterized and found to fit in the general classification of type 1 (CK I) and 2 (CK II) casein kinases, according to their enzymatic and structural properties. Both enzymes phosphorylate acidic substrates, require Mg2+ and have a chromatographic behaviour on DEAE-Sepharose and phosphocellulose similar to their mammalian counterparts. CK I has a sedimentation coefficient of 3.5 S, uses ATP as a phosphate donor (K(m) = 40 μM), phosphorylates casein mainly on serine residues, its activity is strongly inhibited by KCl and polyamines. CK II has a sedimentation coefficient of 7.4 S, uses ATP and GTP as phosphate donors (K(m) ATP = 10 μM; K(m) GTP = 40 μM), phosphorylates casein in serine and threonine, its activity is stimulated by KCl and by polyamines and is inhibited by heparin (I50 = 0.5 μg/ml). Casein kinase activity associated to particulate fraction (40% of total) has been partially characterized and shown to be similar to the soluble CK I activity. |
| author |
Pardo, Patricia Susana Moreno, Silvia |
| author_facet |
Pardo, Patricia Susana Moreno, Silvia |
| author_sort |
Pardo, Patricia Susana |
| title |
Characterization of two casein kinase activities in the fungus Mucor rouxii |
| title_short |
Characterization of two casein kinase activities in the fungus Mucor rouxii |
| title_full |
Characterization of two casein kinase activities in the fungus Mucor rouxii |
| title_fullStr |
Characterization of two casein kinase activities in the fungus Mucor rouxii |
| title_full_unstemmed |
Characterization of two casein kinase activities in the fungus Mucor rouxii |
| title_sort |
characterization of two casein kinase activities in the fungus mucor rouxii |
| publishDate |
1988 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08957479_v12_n4_p183_Pardo http://hdl.handle.net/20.500.12110/paper_08957479_v12_n4_p183_Pardo |
| work_keys_str_mv |
AT pardopatriciasusana characterizationoftwocaseinkinaseactivitiesinthefungusmucorrouxii AT morenosilvia characterizationoftwocaseinkinaseactivitiesinthefungusmucorrouxii |
| _version_ |
1768546403499376640 |