Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens
Antibodies of chronic chagasic patients have been shown to interfere with electric and mechanical activities of cardiac embryonic myocytes in culture and with whole mammalian hearts. A mechanism proposed for this effect involves interaction of the antibodies with G-protein-linked membrane receptors,...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v12_n14_p1551_Masuda http://hdl.handle.net/20.500.12110/paper_08926638_v12_n14_p1551_Masuda |
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paper:paper_08926638_v12_n14_p1551_Masuda2023-06-08T15:47:20Z Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens Heart block Immunoglobulin Muscarinic receptor Ribosomal P proteins guanine nucleotide binding protein ribosome protein animal tissue antigen specificity article atrioventricular conduction cell interaction chagas disease chronic disease gene targeting heart block nonhuman pathogenesis priority journal protein isolation rabbit trypanosoma cruzi Amino Acid Sequence Animals Antigens, Protozoan Autoantibodies Chagas Disease Cross Reactions Electrocardiography Heart Molecular Sequence Data Myocardium Rabbits Receptors, Muscarinic Ribosomal Proteins Trypanosoma cruzi Animalia Mammalia Oryctolagus cuniculus Trypanosoma Trypanosoma cruzi Antibodies of chronic chagasic patients have been shown to interfere with electric and mechanical activities of cardiac embryonic myocytes in culture and with whole mammalian hearts. A mechanism proposed for this effect involves interaction of the antibodies with G-protein-linked membrane receptors, thus leading to activation of beta adrenergic and muscarinic receptors; more specifically, IgG of chagasic patients would interact with the negatively charged regions of the second extracellular loop of these receptors. We performed competition experiments to test this hypothesis. We evaluated the effect of sera/IgG from patients previously known to depress electrogenesis and/or atrioventricular conduction in isolated rabbit hearts after incubation with live and lysed parasites, the peptide corresponding to the second extracellular loop (O2) of the M2 receptor, and different peptides derived from two ribosomal proteins of T. cruzi: P0 and P2β. Our results indicate that 1) the antigenic factor inducing the functionally active IgGs in the chagasic patients is probably an intracellular T. cruzi antigen; 2) IgG/serum is interacting with the O2 region of the M2 receptor in the rabbit heart; and 3) the negative charges present in the ribosomal proteins of T. cruzi are important in mediating the interaction between the patients' serum/IgG and the receptor. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v12_n14_p1551_Masuda http://hdl.handle.net/20.500.12110/paper_08926638_v12_n14_p1551_Masuda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Heart block Immunoglobulin Muscarinic receptor Ribosomal P proteins guanine nucleotide binding protein ribosome protein animal tissue antigen specificity article atrioventricular conduction cell interaction chagas disease chronic disease gene targeting heart block nonhuman pathogenesis priority journal protein isolation rabbit trypanosoma cruzi Amino Acid Sequence Animals Antigens, Protozoan Autoantibodies Chagas Disease Cross Reactions Electrocardiography Heart Molecular Sequence Data Myocardium Rabbits Receptors, Muscarinic Ribosomal Proteins Trypanosoma cruzi Animalia Mammalia Oryctolagus cuniculus Trypanosoma Trypanosoma cruzi |
| spellingShingle |
Heart block Immunoglobulin Muscarinic receptor Ribosomal P proteins guanine nucleotide binding protein ribosome protein animal tissue antigen specificity article atrioventricular conduction cell interaction chagas disease chronic disease gene targeting heart block nonhuman pathogenesis priority journal protein isolation rabbit trypanosoma cruzi Amino Acid Sequence Animals Antigens, Protozoan Autoantibodies Chagas Disease Cross Reactions Electrocardiography Heart Molecular Sequence Data Myocardium Rabbits Receptors, Muscarinic Ribosomal Proteins Trypanosoma cruzi Animalia Mammalia Oryctolagus cuniculus Trypanosoma Trypanosoma cruzi Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens |
| topic_facet |
Heart block Immunoglobulin Muscarinic receptor Ribosomal P proteins guanine nucleotide binding protein ribosome protein animal tissue antigen specificity article atrioventricular conduction cell interaction chagas disease chronic disease gene targeting heart block nonhuman pathogenesis priority journal protein isolation rabbit trypanosoma cruzi Amino Acid Sequence Animals Antigens, Protozoan Autoantibodies Chagas Disease Cross Reactions Electrocardiography Heart Molecular Sequence Data Myocardium Rabbits Receptors, Muscarinic Ribosomal Proteins Trypanosoma cruzi Animalia Mammalia Oryctolagus cuniculus Trypanosoma Trypanosoma cruzi |
| description |
Antibodies of chronic chagasic patients have been shown to interfere with electric and mechanical activities of cardiac embryonic myocytes in culture and with whole mammalian hearts. A mechanism proposed for this effect involves interaction of the antibodies with G-protein-linked membrane receptors, thus leading to activation of beta adrenergic and muscarinic receptors; more specifically, IgG of chagasic patients would interact with the negatively charged regions of the second extracellular loop of these receptors. We performed competition experiments to test this hypothesis. We evaluated the effect of sera/IgG from patients previously known to depress electrogenesis and/or atrioventricular conduction in isolated rabbit hearts after incubation with live and lysed parasites, the peptide corresponding to the second extracellular loop (O2) of the M2 receptor, and different peptides derived from two ribosomal proteins of T. cruzi: P0 and P2β. Our results indicate that 1) the antigenic factor inducing the functionally active IgGs in the chagasic patients is probably an intracellular T. cruzi antigen; 2) IgG/serum is interacting with the O2 region of the M2 receptor in the rabbit heart; and 3) the negative charges present in the ribosomal proteins of T. cruzi are important in mediating the interaction between the patients' serum/IgG and the receptor. |
| title |
Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens |
| title_short |
Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens |
| title_full |
Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens |
| title_fullStr |
Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens |
| title_full_unstemmed |
Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens |
| title_sort |
functionally active cardiac antibodies in chronic chagas' disease are specifically blocked by trypanosoma cruzi antigens |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v12_n14_p1551_Masuda http://hdl.handle.net/20.500.12110/paper_08926638_v12_n14_p1551_Masuda |
| _version_ |
1768542794270375936 |