Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157: H7 and non-O157 biofilms
Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens, with the main virulence factor of this bacterium being its capacity to secrete Shiga toxins (Stxs). Therefore, the use of certain antibiotics for the treatment of this infection, which induces the liberation of Stxs, i...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v29_n7_p1692_AngelVillegas http://hdl.handle.net/20.500.12110/paper_08872333_v29_n7_p1692_AngelVillegas |
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paper:paper_08872333_v29_n7_p1692_AngelVillegas2023-06-08T15:46:45Z Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157: H7 and non-O157 biofilms Antibiotic Biofilms Ciprofloxacin Escherichia coli O157:H7 Fosfomycin Hemolytic uremic syndrome (HUS) Rifaximin Shiga toxin (Stx) antibiotic agent antioxidant ascorbic acid catalase ciprofloxacin fosfomycin glutathione nitrite oxidizing agent reactive oxygen metabolite rifaximin Shiga toxin superoxide dismutase tiron antiinfective agent antioxidant ascorbic acid catalase ciprofloxacin fosfomycin glutathione nitric oxide reactive oxygen metabolite rifamycin rifaximin superoxide dismutase verotoxin 1 verotoxin 2 virulence factor antibiotic resistance antibiotic sensitivity antioxidant activity antioxidant responsive element Article bacterial strain bacterial virulence biofilm cell stress controlled study cytotoxicity drug effect enzyme activity Escherichia coli O157 metabolite minimum inhibitory concentration nonhuman oxidative stress Vero cell line animal biofilm cell survival Chlorocebus aethiops drug effects genetics metabolism microbial sensitivity test physiology Shiga toxin producing Escherichia coli Bacteria (microorganisms) Escherichia coli Animals Anti-Bacterial Agents Antioxidants Ascorbic Acid Biofilms Catalase Cell Survival Cercopithecus aethiops Ciprofloxacin Fosfomycin Glutathione Microbial Sensitivity Tests Nitric Oxide Oxidative Stress Reactive Oxygen Species Rifamycins Shiga Toxin 1 Shiga Toxin 2 Shiga-Toxigenic Escherichia coli Superoxide Dismutase Vero Cells Virulence Factors Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens, with the main virulence factor of this bacterium being its capacity to secrete Shiga toxins (Stxs). Therefore, the use of certain antibiotics for the treatment of this infection, which induces the liberation of Stxs, is controversial. Reactive oxygen and nitrogen species are also involved in the pathogenesis of different diseases. The purpose of this study was to analyze the effects of antibiotics on biofilms of STEC and the relationships between cellular stress and the release of Stx. To this end, biofilms of reference and clinical strains were treated with antibiotics (ciprofloxacin, fosfomycin and rifaximin) and the production of oxidants, the antioxidant defense system and toxin release were evaluated. Ciprofloxacin altered the prooxidant-antioxidant balance, with a decrease of oxidant metabolites and an increase of superoxide dismutase and catalase activity, being associated with high-levels of Stx production. Furthermore, inhibition of oxidative stress by exogenous antioxidants was correlated with a reduction in the liberation of Stx, indicating the participation of this phenomenon in the release of this toxin. In contrast, fosfomycin and rifaximin produced less alteration with a minimal production of Stx. Our data show that treatment of biofilm-STEC with these antibiotics induces oxidative stress-mediated release of Stx. © 2015 Elsevier Ltd. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v29_n7_p1692_AngelVillegas http://hdl.handle.net/20.500.12110/paper_08872333_v29_n7_p1692_AngelVillegas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antibiotic Biofilms Ciprofloxacin Escherichia coli O157:H7 Fosfomycin Hemolytic uremic syndrome (HUS) Rifaximin Shiga toxin (Stx) antibiotic agent antioxidant ascorbic acid catalase ciprofloxacin fosfomycin glutathione nitrite oxidizing agent reactive oxygen metabolite rifaximin Shiga toxin superoxide dismutase tiron antiinfective agent antioxidant ascorbic acid catalase ciprofloxacin fosfomycin glutathione nitric oxide reactive oxygen metabolite rifamycin rifaximin superoxide dismutase verotoxin 1 verotoxin 2 virulence factor antibiotic resistance antibiotic sensitivity antioxidant activity antioxidant responsive element Article bacterial strain bacterial virulence biofilm cell stress controlled study cytotoxicity drug effect enzyme activity Escherichia coli O157 metabolite minimum inhibitory concentration nonhuman oxidative stress Vero cell line animal biofilm cell survival Chlorocebus aethiops drug effects genetics metabolism microbial sensitivity test physiology Shiga toxin producing Escherichia coli Bacteria (microorganisms) Escherichia coli Animals Anti-Bacterial Agents Antioxidants Ascorbic Acid Biofilms Catalase Cell Survival Cercopithecus aethiops Ciprofloxacin Fosfomycin Glutathione Microbial Sensitivity Tests Nitric Oxide Oxidative Stress Reactive Oxygen Species Rifamycins Shiga Toxin 1 Shiga Toxin 2 Shiga-Toxigenic Escherichia coli Superoxide Dismutase Vero Cells Virulence Factors |
| spellingShingle |
Antibiotic Biofilms Ciprofloxacin Escherichia coli O157:H7 Fosfomycin Hemolytic uremic syndrome (HUS) Rifaximin Shiga toxin (Stx) antibiotic agent antioxidant ascorbic acid catalase ciprofloxacin fosfomycin glutathione nitrite oxidizing agent reactive oxygen metabolite rifaximin Shiga toxin superoxide dismutase tiron antiinfective agent antioxidant ascorbic acid catalase ciprofloxacin fosfomycin glutathione nitric oxide reactive oxygen metabolite rifamycin rifaximin superoxide dismutase verotoxin 1 verotoxin 2 virulence factor antibiotic resistance antibiotic sensitivity antioxidant activity antioxidant responsive element Article bacterial strain bacterial virulence biofilm cell stress controlled study cytotoxicity drug effect enzyme activity Escherichia coli O157 metabolite minimum inhibitory concentration nonhuman oxidative stress Vero cell line animal biofilm cell survival Chlorocebus aethiops drug effects genetics metabolism microbial sensitivity test physiology Shiga toxin producing Escherichia coli Bacteria (microorganisms) Escherichia coli Animals Anti-Bacterial Agents Antioxidants Ascorbic Acid Biofilms Catalase Cell Survival Cercopithecus aethiops Ciprofloxacin Fosfomycin Glutathione Microbial Sensitivity Tests Nitric Oxide Oxidative Stress Reactive Oxygen Species Rifamycins Shiga Toxin 1 Shiga Toxin 2 Shiga-Toxigenic Escherichia coli Superoxide Dismutase Vero Cells Virulence Factors Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157: H7 and non-O157 biofilms |
| topic_facet |
Antibiotic Biofilms Ciprofloxacin Escherichia coli O157:H7 Fosfomycin Hemolytic uremic syndrome (HUS) Rifaximin Shiga toxin (Stx) antibiotic agent antioxidant ascorbic acid catalase ciprofloxacin fosfomycin glutathione nitrite oxidizing agent reactive oxygen metabolite rifaximin Shiga toxin superoxide dismutase tiron antiinfective agent antioxidant ascorbic acid catalase ciprofloxacin fosfomycin glutathione nitric oxide reactive oxygen metabolite rifamycin rifaximin superoxide dismutase verotoxin 1 verotoxin 2 virulence factor antibiotic resistance antibiotic sensitivity antioxidant activity antioxidant responsive element Article bacterial strain bacterial virulence biofilm cell stress controlled study cytotoxicity drug effect enzyme activity Escherichia coli O157 metabolite minimum inhibitory concentration nonhuman oxidative stress Vero cell line animal biofilm cell survival Chlorocebus aethiops drug effects genetics metabolism microbial sensitivity test physiology Shiga toxin producing Escherichia coli Bacteria (microorganisms) Escherichia coli Animals Anti-Bacterial Agents Antioxidants Ascorbic Acid Biofilms Catalase Cell Survival Cercopithecus aethiops Ciprofloxacin Fosfomycin Glutathione Microbial Sensitivity Tests Nitric Oxide Oxidative Stress Reactive Oxygen Species Rifamycins Shiga Toxin 1 Shiga Toxin 2 Shiga-Toxigenic Escherichia coli Superoxide Dismutase Vero Cells Virulence Factors |
| description |
Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens, with the main virulence factor of this bacterium being its capacity to secrete Shiga toxins (Stxs). Therefore, the use of certain antibiotics for the treatment of this infection, which induces the liberation of Stxs, is controversial. Reactive oxygen and nitrogen species are also involved in the pathogenesis of different diseases. The purpose of this study was to analyze the effects of antibiotics on biofilms of STEC and the relationships between cellular stress and the release of Stx. To this end, biofilms of reference and clinical strains were treated with antibiotics (ciprofloxacin, fosfomycin and rifaximin) and the production of oxidants, the antioxidant defense system and toxin release were evaluated. Ciprofloxacin altered the prooxidant-antioxidant balance, with a decrease of oxidant metabolites and an increase of superoxide dismutase and catalase activity, being associated with high-levels of Stx production. Furthermore, inhibition of oxidative stress by exogenous antioxidants was correlated with a reduction in the liberation of Stx, indicating the participation of this phenomenon in the release of this toxin. In contrast, fosfomycin and rifaximin produced less alteration with a minimal production of Stx. Our data show that treatment of biofilm-STEC with these antibiotics induces oxidative stress-mediated release of Stx. © 2015 Elsevier Ltd. |
| title |
Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157: H7 and non-O157 biofilms |
| title_short |
Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157: H7 and non-O157 biofilms |
| title_full |
Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157: H7 and non-O157 biofilms |
| title_fullStr |
Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157: H7 and non-O157 biofilms |
| title_full_unstemmed |
Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157: H7 and non-O157 biofilms |
| title_sort |
effect of antibiotics on cellular stress generated in shiga toxin-producing escherichia coli o157: h7 and non-o157 biofilms |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08872333_v29_n7_p1692_AngelVillegas http://hdl.handle.net/20.500.12110/paper_08872333_v29_n7_p1692_AngelVillegas |
| _version_ |
1768543377222008832 |