The genomic composition of Tricepiro, a synthetic forage crop

Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid tri...

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Autores principales:	Ferrari, María Rosa, Greizerstein, Eduardo José, Naranjo, Carlos Alberto, Poggio, Lidia
Publicado:	2005
Materias:	FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale cereale Triticosecale Triticum aestivum
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v48_n1_p154_Ferrari http://hdl.handle.net/20.500.12110/paper_08312796_v48_n1_p154_Ferrari
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_08312796_v48_n1_p154_Ferrari
record_format	dspace
spelling	paper:paper_08312796_v48_n1_p154_Ferrari2023-06-08T15:46:16Z The genomic composition of Tricepiro, a synthetic forage crop Ferrari, María Rosa Greizerstein, Eduardo José Naranjo, Carlos Alberto Poggio, Lidia FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale Secale cereale Thinopyrum Triticosecale Triticum Triticum aestivum Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid triticale (2n = 6x = 42) and octoploid Trigopiro (2n = 8x = 56). Several years of breeding produced a forage crop with valuable characteristics from Secale, Triticum, and Thinopyrum. The aim of this work is to analyse the real genomic constitution of this important synthetic crop. In situ hybridization using total DNA of Secale, Triticum, and Thinopyrum as a probe (GISH) labelled with biotin and (or) digoxigenin showed that tricepiro is composed of 14 rye chromosomes and 28 wheat chromosomes. Small zones of introgression of Thinopyrum on wheat chromosomes were detected. The FISH using the rye repetitive DNA probe pSc 119.2 labelled with biotin let us characterize the seven pairs of rye chromosomes. Moreover, several wheat chromosomes belonging to A and B genomes were distinguished. Therefore, tricepiro is a synthetic hexaploid (2n = 6x = 42) being AABBRR in its genomic composition, with zones of introgression of Thinopyrum in the A genome of wheat. © 2005 NRC Canada. Fil:Ferrari, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Greizerstein, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Naranjo, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v48_n1_p154_Ferrari http://hdl.handle.net/20.500.12110/paper_08312796_v48_n1_p154_Ferrari
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale Secale cereale Thinopyrum Triticosecale Triticum Triticum aestivum
spellingShingle	FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale Secale cereale Thinopyrum Triticosecale Triticum Triticum aestivum Ferrari, María Rosa Greizerstein, Eduardo José Naranjo, Carlos Alberto Poggio, Lidia The genomic composition of Tricepiro, a synthetic forage crop
topic_facet	FISH Genomic composition GISH In situ hybridization Secale Synthetic forage crop Thinopyrum Tricepiro Trihybrid Triticum article cereal fluorescence in situ hybridization genetics genome mitosis plant chromosome Cereals Chromosomes, Plant Genome, Plant In Situ Hybridization, Fluorescence Mitosis Secale Secale cereale Thinopyrum Triticosecale Triticum Triticum aestivum
description	Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid triticale (2n = 6x = 42) and octoploid Trigopiro (2n = 8x = 56). Several years of breeding produced a forage crop with valuable characteristics from Secale, Triticum, and Thinopyrum. The aim of this work is to analyse the real genomic constitution of this important synthetic crop. In situ hybridization using total DNA of Secale, Triticum, and Thinopyrum as a probe (GISH) labelled with biotin and (or) digoxigenin showed that tricepiro is composed of 14 rye chromosomes and 28 wheat chromosomes. Small zones of introgression of Thinopyrum on wheat chromosomes were detected. The FISH using the rye repetitive DNA probe pSc 119.2 labelled with biotin let us characterize the seven pairs of rye chromosomes. Moreover, several wheat chromosomes belonging to A and B genomes were distinguished. Therefore, tricepiro is a synthetic hexaploid (2n = 6x = 42) being AABBRR in its genomic composition, with zones of introgression of Thinopyrum in the A genome of wheat. © 2005 NRC Canada.
author	Ferrari, María Rosa Greizerstein, Eduardo José Naranjo, Carlos Alberto Poggio, Lidia
author_facet	Ferrari, María Rosa Greizerstein, Eduardo José Naranjo, Carlos Alberto Poggio, Lidia
author_sort	Ferrari, María Rosa
title	The genomic composition of Tricepiro, a synthetic forage crop
title_short	The genomic composition of Tricepiro, a synthetic forage crop
title_full	The genomic composition of Tricepiro, a synthetic forage crop
title_fullStr	The genomic composition of Tricepiro, a synthetic forage crop
title_full_unstemmed	The genomic composition of Tricepiro, a synthetic forage crop
title_sort	genomic composition of tricepiro, a synthetic forage crop
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v48_n1_p154_Ferrari http://hdl.handle.net/20.500.12110/paper_08312796_v48_n1_p154_Ferrari
work_keys_str_mv	AT ferrarimariarosa thegenomiccompositionoftricepiroasyntheticforagecrop AT greizersteineduardojose thegenomiccompositionoftricepiroasyntheticforagecrop AT naranjocarlosalberto thegenomiccompositionoftricepiroasyntheticforagecrop AT poggiolidia thegenomiccompositionoftricepiroasyntheticforagecrop AT ferrarimariarosa genomiccompositionoftricepiroasyntheticforagecrop AT greizersteineduardojose genomiccompositionoftricepiroasyntheticforagecrop AT naranjocarlosalberto genomiccompositionoftricepiroasyntheticforagecrop AT poggiolidia genomiccompositionoftricepiroasyntheticforagecrop
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The genomic composition of Tricepiro, a synthetic forage crop

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