Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes
Genomic affinities between Tripsacum dactyloides (2n = 72) and Zea mays ssp. mays (2n = 20 + 5 B) were analyzed through GISH (genomic in situ hybridization) to ascertain the degree of chromosome homology between the two genera. Mitotic cells of T. dactyloides were simultaneously probed with total ge...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n4_p687_Poggio http://hdl.handle.net/20.500.12110/paper_08312796_v42_n4_p687_Poggio |
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paper:paper_08312796_v42_n4_p687_Poggio2023-06-08T15:46:14Z Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes Genomic in situ hybridization GISH Maize B chromosomes Tripsacum dactyloides Zea mays ssp. mays chromosomal localization DNA probe fluorescence genome analysis genomic in situ hybridization in situ hybridization mitosis ribosomal DNA Tripsacum dactyloides Zea mays Genomic affinities between Tripsacum dactyloides (2n = 72) and Zea mays ssp. mays (2n = 20 + 5 B) were analyzed through GISH (genomic in situ hybridization) to ascertain the degree of chromosome homology between the two genera. Mitotic cells of T. dactyloides were simultaneously probed with total genomic DNA from Z. mays ssp. mays (2n = 20) and with rDNA (pTA71). A disperse pattern of hybridization signal among all 72 chromosomes, corresponding to maize total DNA, and six strong fluorescent signals due to the rDNA probe hybridizing on 3 chromosome pairs of T. dactyloides were observed. Mitotic chromosomes from Z. mays ssp. mays (2n = 20 + 5 B) were hybridized with a maize line that lacked B chromosomes and knobs and with total DNA from T. dactyloides. The knobless line of maize hybridized intensely on all chromosomes except for some regions where the probe bound less. Tripsacum dactyloides bound intensely on one terminal region of each B chromosome and to some regions of chromosome pairs 2, 6, and 8. These regions are DAPI positive and coincide with regions that displayed lower affinity with the probe from the knobless maize line. The possible significance of these results is discussed briefly. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n4_p687_Poggio http://hdl.handle.net/20.500.12110/paper_08312796_v42_n4_p687_Poggio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Genomic in situ hybridization GISH Maize B chromosomes Tripsacum dactyloides Zea mays ssp. mays chromosomal localization DNA probe fluorescence genome analysis genomic in situ hybridization in situ hybridization mitosis ribosomal DNA Tripsacum dactyloides Zea mays |
| spellingShingle |
Genomic in situ hybridization GISH Maize B chromosomes Tripsacum dactyloides Zea mays ssp. mays chromosomal localization DNA probe fluorescence genome analysis genomic in situ hybridization in situ hybridization mitosis ribosomal DNA Tripsacum dactyloides Zea mays Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes |
| topic_facet |
Genomic in situ hybridization GISH Maize B chromosomes Tripsacum dactyloides Zea mays ssp. mays chromosomal localization DNA probe fluorescence genome analysis genomic in situ hybridization in situ hybridization mitosis ribosomal DNA Tripsacum dactyloides Zea mays |
| description |
Genomic affinities between Tripsacum dactyloides (2n = 72) and Zea mays ssp. mays (2n = 20 + 5 B) were analyzed through GISH (genomic in situ hybridization) to ascertain the degree of chromosome homology between the two genera. Mitotic cells of T. dactyloides were simultaneously probed with total genomic DNA from Z. mays ssp. mays (2n = 20) and with rDNA (pTA71). A disperse pattern of hybridization signal among all 72 chromosomes, corresponding to maize total DNA, and six strong fluorescent signals due to the rDNA probe hybridizing on 3 chromosome pairs of T. dactyloides were observed. Mitotic chromosomes from Z. mays ssp. mays (2n = 20 + 5 B) were hybridized with a maize line that lacked B chromosomes and knobs and with total DNA from T. dactyloides. The knobless line of maize hybridized intensely on all chromosomes except for some regions where the probe bound less. Tripsacum dactyloides bound intensely on one terminal region of each B chromosome and to some regions of chromosome pairs 2, 6, and 8. These regions are DAPI positive and coincide with regions that displayed lower affinity with the probe from the knobless maize line. The possible significance of these results is discussed briefly. |
| title |
Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes |
| title_short |
Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes |
| title_full |
Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes |
| title_fullStr |
Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes |
| title_full_unstemmed |
Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes |
| title_sort |
genomic in situ hybridization (gish) of tripsacum dactyloides and zea mays ssp. mays with b chromosomes |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v42_n4_p687_Poggio http://hdl.handle.net/20.500.12110/paper_08312796_v42_n4_p687_Poggio |
| _version_ |
1768543377031168000 |