cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.

We examined the mechanism underlying the effect of cAMP on delta-aminolevulinate synthase mRNA biosynthesis in isolated hepatocytes from normal and experimental diabetic rats. We have demonstrated that the potentiation by dibutyryl cAMP of the phenobarbital-mediated induction of delta-aminolevulinat...

Descripción completa

Detalles Bibliográficos
Autores principales: Varone, Cecilia Laura, Cánepa, Eduardo Tomás, Llambías, Elena Blanca Cecilia, Grinstein, Moisés
Publicado: 1994
Materias:
5 aminolevulinate synthase
bucladesine
cyclic AMP
messenger RNA
phenobarbital
animal
article
biosynthesis
dose response
experimental diabetes mellitus
gene expression regulation
genetics
half life time
in vitro study
liver
male
metabolism
molecular genetics
Northern blotting
nucleotide sequence
rat
rat strain
time
5-Aminolevulinate Synthetase
Animals
Base Sequence
Blotting, Northern
Bucladesine
Cyclic AMP
Diabetes Mellitus, Experimental
Dose-Response Relationship, Drug
Gene Expression Regulation, Enzymologic
Half-Life
Liver
Male
Molecular Sequence Data
Phenobarbital
Rats
Rats, Inbred Strains
RNA, Messenger
Time Factors
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v72_n9-10_p381_Varone
http://hdl.handle.net/20.500.12110/paper_08298211_v72_n9-10_p381_Varone
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_08298211_v72_n9-10_p381_Varone
record_format dspace
spelling paper:paper_08298211_v72_n9-10_p381_Varone2023-06-08T15:46:13Z cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats. Varone, Cecilia Laura Cánepa, Eduardo Tomás Llambías, Elena Blanca Cecilia Grinstein, Moisés 5 aminolevulinate synthase bucladesine cyclic AMP messenger RNA phenobarbital animal article biosynthesis dose response experimental diabetes mellitus gene expression regulation genetics half life time in vitro study liver male metabolism molecular genetics Northern blotting nucleotide sequence rat rat strain time 5-Aminolevulinate Synthetase Animals Base Sequence Blotting, Northern Bucladesine Cyclic AMP Diabetes Mellitus, Experimental Dose-Response Relationship, Drug Gene Expression Regulation, Enzymologic Half-Life Liver Male Molecular Sequence Data Phenobarbital Rats Rats, Inbred Strains RNA, Messenger Time Factors We examined the mechanism underlying the effect of cAMP on delta-aminolevulinate synthase mRNA biosynthesis in isolated hepatocytes from normal and experimental diabetic rats. We have demonstrated that the potentiation by dibutyryl cAMP of the phenobarbital-mediated induction of delta-aminolevulinate synthase enzyme activity, observed in our previously reported studies, reflects an increased amount of its mRNA. The inducing effect exerted by phenobarbital on the biosynthesis of delta-aminolevulinate synthase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response to the increased level of endogenous cAMP in diabetic hepatocytes is apparently sufficient for a maximum activation of the cAMP-dependent protein kinase. The present results suggest that in rat liver dibutyryl cAMP modulates delta-aminolevulinate synthase mRNA biosynthesis by acting predominantly, if not exclusively, at the level of gene transcription. Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Canépa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambías, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v72_n9-10_p381_Varone http://hdl.handle.net/20.500.12110/paper_08298211_v72_n9-10_p381_Varone
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 5 aminolevulinate synthase
bucladesine
cyclic AMP
messenger RNA
phenobarbital
animal
article
biosynthesis
dose response
experimental diabetes mellitus
gene expression regulation
genetics
half life time
in vitro study
liver
male
metabolism
molecular genetics
Northern blotting
nucleotide sequence
rat
rat strain
time
5-Aminolevulinate Synthetase
Animals
Base Sequence
Blotting, Northern
Bucladesine
Cyclic AMP
Diabetes Mellitus, Experimental
Dose-Response Relationship, Drug
Gene Expression Regulation, Enzymologic
Half-Life
Liver
Male
Molecular Sequence Data
Phenobarbital
Rats
Rats, Inbred Strains
RNA, Messenger
Time Factors
spellingShingle 5 aminolevulinate synthase
bucladesine
cyclic AMP
messenger RNA
phenobarbital
animal
article
biosynthesis
dose response
experimental diabetes mellitus
gene expression regulation
genetics
half life time
in vitro study
liver
male
metabolism
molecular genetics
Northern blotting
nucleotide sequence
rat
rat strain
time
5-Aminolevulinate Synthetase
Animals
Base Sequence
Blotting, Northern
Bucladesine
Cyclic AMP
Diabetes Mellitus, Experimental
Dose-Response Relationship, Drug
Gene Expression Regulation, Enzymologic
Half-Life
Liver
Male
Molecular Sequence Data
Phenobarbital
Rats
Rats, Inbred Strains
RNA, Messenger
Time Factors
Varone, Cecilia Laura
Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.
topic_facet 5 aminolevulinate synthase
bucladesine
cyclic AMP
messenger RNA
phenobarbital
animal
article
biosynthesis
dose response
experimental diabetes mellitus
gene expression regulation
genetics
half life time
in vitro study
liver
male
metabolism
molecular genetics
Northern blotting
nucleotide sequence
rat
rat strain
time
5-Aminolevulinate Synthetase
Animals
Base Sequence
Blotting, Northern
Bucladesine
Cyclic AMP
Diabetes Mellitus, Experimental
Dose-Response Relationship, Drug
Gene Expression Regulation, Enzymologic
Half-Life
Liver
Male
Molecular Sequence Data
Phenobarbital
Rats
Rats, Inbred Strains
RNA, Messenger
Time Factors
description We examined the mechanism underlying the effect of cAMP on delta-aminolevulinate synthase mRNA biosynthesis in isolated hepatocytes from normal and experimental diabetic rats. We have demonstrated that the potentiation by dibutyryl cAMP of the phenobarbital-mediated induction of delta-aminolevulinate synthase enzyme activity, observed in our previously reported studies, reflects an increased amount of its mRNA. The inducing effect exerted by phenobarbital on the biosynthesis of delta-aminolevulinate synthase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response to the increased level of endogenous cAMP in diabetic hepatocytes is apparently sufficient for a maximum activation of the cAMP-dependent protein kinase. The present results suggest that in rat liver dibutyryl cAMP modulates delta-aminolevulinate synthase mRNA biosynthesis by acting predominantly, if not exclusively, at the level of gene transcription.
author Varone, Cecilia Laura
Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
author_facet Varone, Cecilia Laura
Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
author_sort Varone, Cecilia Laura
title cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.
title_short cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.
title_full cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.
title_fullStr cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.
title_full_unstemmed cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.
title_sort camp regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mrna in hepatocytes from normal and experimental diabetic rats.
publishDate 1994
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v72_n9-10_p381_Varone
http://hdl.handle.net/20.500.12110/paper_08298211_v72_n9-10_p381_Varone
work_keys_str_mv AT varonececilialaura campregulationofphenobarbitalmediatedinductionofdeltaaminolevulinatesynthasemrnainhepatocytesfromnormalandexperimentaldiabeticrats
AT canepaeduardotomas campregulationofphenobarbitalmediatedinductionofdeltaaminolevulinatesynthasemrnainhepatocytesfromnormalandexperimentaldiabeticrats
AT llambiaselenablancacecilia campregulationofphenobarbitalmediatedinductionofdeltaaminolevulinatesynthasemrnainhepatocytesfromnormalandexperimentaldiabeticrats
AT grinsteinmoises campregulationofphenobarbitalmediatedinductionofdeltaaminolevulinatesynthasemrnainhepatocytesfromnormalandexperimentaldiabeticrats
_version_ 1768543041136623616

Ejemplares similares