Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii
Molecular genetic analysis was applied to 162 individuals of 37 half-sib selected families belonging to six provenances of Eucalyptus dunnii Maiden in a provenance/family trial. The individuals were selected by a trait selection index and genetic diversity criteria were later applied for designing s...
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paper:paper_0829318X_v25_n11_p1457_Zelener2023-06-08T15:46:06Z Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii Marcucci Poltri, Susana N. Hopp, Horacio Esteban AFLP AMOVA DNA fingerprinting Forest tree improvement Genetic diversity Genetic relationship Microsatellites Population genetic structure Simple sequence repeats (SSRs) DNA fingerprinting genetic variation plant breeding provenance trial silviculture Eucalyptus Eucalyptus dunnii Molecular genetic analysis was applied to 162 individuals of 37 half-sib selected families belonging to six provenances of Eucalyptus dunnii Maiden in a provenance/family trial. The individuals were selected by a trait selection index and genetic diversity criteria were later applied for designing seedling seed orchards. Genetic diversity and its distribution, as well as relationships among individuals, were assessed on the basis of nine microsatellite loci and 243 amplified fragment length polymorphism markers. High diversity was found with both kinds of markers. Clear-cut genomic patterns of identification (fingerprinting) were obtained for each individual. Genetic differentiation estimates consistently showed low differentiation among provenances (R ST1 = 0.069, θP = 0.026 and FCT = 0.035) and great differentiation among families (RST2 = 0.223, θS = 0.174 and FSC = 0.164). A high proportion of the total variation was observed within families (around 80% by both marker analyses), suggesting that orchard design should be based on individual or family selection rather than on provenance selection, and that individual ranking by both trait selection index and molecular genetic diversity criteria should be considered. A selection procedure for a seedling seed orchard design is proposed. © 2005 Heron Publishing. Fil:Marcucci Poltri, S.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0829318X_v25_n11_p1457_Zelener http://hdl.handle.net/20.500.12110/paper_0829318X_v25_n11_p1457_Zelener |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
AFLP AMOVA DNA fingerprinting Forest tree improvement Genetic diversity Genetic relationship Microsatellites Population genetic structure Simple sequence repeats (SSRs) DNA fingerprinting genetic variation plant breeding provenance trial silviculture Eucalyptus Eucalyptus dunnii |
spellingShingle |
AFLP AMOVA DNA fingerprinting Forest tree improvement Genetic diversity Genetic relationship Microsatellites Population genetic structure Simple sequence repeats (SSRs) DNA fingerprinting genetic variation plant breeding provenance trial silviculture Eucalyptus Eucalyptus dunnii Marcucci Poltri, Susana N. Hopp, Horacio Esteban Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii |
topic_facet |
AFLP AMOVA DNA fingerprinting Forest tree improvement Genetic diversity Genetic relationship Microsatellites Population genetic structure Simple sequence repeats (SSRs) DNA fingerprinting genetic variation plant breeding provenance trial silviculture Eucalyptus Eucalyptus dunnii |
description |
Molecular genetic analysis was applied to 162 individuals of 37 half-sib selected families belonging to six provenances of Eucalyptus dunnii Maiden in a provenance/family trial. The individuals were selected by a trait selection index and genetic diversity criteria were later applied for designing seedling seed orchards. Genetic diversity and its distribution, as well as relationships among individuals, were assessed on the basis of nine microsatellite loci and 243 amplified fragment length polymorphism markers. High diversity was found with both kinds of markers. Clear-cut genomic patterns of identification (fingerprinting) were obtained for each individual. Genetic differentiation estimates consistently showed low differentiation among provenances (R ST1 = 0.069, θP = 0.026 and FCT = 0.035) and great differentiation among families (RST2 = 0.223, θS = 0.174 and FSC = 0.164). A high proportion of the total variation was observed within families (around 80% by both marker analyses), suggesting that orchard design should be based on individual or family selection rather than on provenance selection, and that individual ranking by both trait selection index and molecular genetic diversity criteria should be considered. A selection procedure for a seedling seed orchard design is proposed. © 2005 Heron Publishing. |
author |
Marcucci Poltri, Susana N. Hopp, Horacio Esteban |
author_facet |
Marcucci Poltri, Susana N. Hopp, Horacio Esteban |
author_sort |
Marcucci Poltri, Susana N. |
title |
Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii |
title_short |
Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii |
title_full |
Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii |
title_fullStr |
Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii |
title_full_unstemmed |
Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: A case study for Eucalyptus dunnii |
title_sort |
selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: a case study for eucalyptus dunnii |
publishDate |
2005 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0829318X_v25_n11_p1457_Zelener http://hdl.handle.net/20.500.12110/paper_0829318X_v25_n11_p1457_Zelener |
work_keys_str_mv |
AT marcuccipoltrisusanan selectionstrategyforaseedlingseedorcharddesignbasedontraitselectionindexandgenomicanalysisbymolecularmarkersacasestudyforeucalyptusdunnii AT hopphoracioesteban selectionstrategyforaseedlingseedorcharddesignbasedontraitselectionindexandgenomicanalysisbymolecularmarkersacasestudyforeucalyptusdunnii |
_version_ |
1768541942957735936 |