Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers

A Eucalyptus dunnii Maiden breeding population of 46 accessions originated in Australia and selected for fitness to subtropical and cold environments was screened by Amplified Fragment Length Polymorphism (AFLP) and microsatellite markers to obtain quantitative estimates of genetic diversity. A rand...

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Autores principales:	Marcucci Poltri, Susana N., Hopp, Horacio Esteban
Publicado:	2003
Materias:	AFLP Forest tree breeding Germplasm Identification matrix Microsatellite Simple sequence repeats SSR genetic variation seed Alleles Eucalyptus Forestry Genetic Markers Genotype Microsatellite Repeats Random Amplified Polymorphic DNA Technique Seeds Trees Variation (Genetics) Eucalyptus dunnii
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0829318X_v23_n9_p625_MarcucciPoltri http://hdl.handle.net/20.500.12110/paper_0829318X_v23_n9_p625_MarcucciPoltri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0829318X_v23_n9_p625_MarcucciPoltri
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spelling	paper:paper_0829318X_v23_n9_p625_MarcucciPoltri2023-06-08T15:46:05Z Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers Marcucci Poltri, Susana N. Hopp, Horacio Esteban AFLP Forest tree breeding Germplasm Identification matrix Microsatellite Simple sequence repeats SSR genetic variation seed Alleles Eucalyptus Forestry Genetic Markers Genotype Microsatellite Repeats Random Amplified Polymorphic DNA Technique Seeds Trees Variation (Genetics) Eucalyptus Eucalyptus dunnii A Eucalyptus dunnii Maiden breeding population of 46 accessions originated in Australia and selected for fitness to subtropical and cold environments was screened by Amplified Fragment Length Polymorphism (AFLP) and microsatellite markers to obtain quantitative estimates of genetic diversity. A randomly chosen group of AFLP primers generated 205 AFLP bands that were used to fingerprint the genotypes and to evaluate genetic relationships among accessions. Sixty-eight percent (140) of the bands were polymorphic markers. The mean diversity index (DI) was 0.33 and about 52% of the loci had values greater than 0.4. Cluster analysis derived from similarity indices (SI) revealed no particular grouping among accessions suggesting the absence of closely related genotypes, except for five pairs of genotypes. Bootstrap analysis results confirmed the suitability of AFLP to describe genetic relationships in this breeding population. In addition, four highly informative microsatellites were used to construct an identification matrix that discriminated nearly all of the genotypes. Mean values for the number of alleles per locus, DI and SI among accessions were 13, 0.78 and 0.19, respectively, indicating that the breeding population has high genetic diversity. However, several genotypes showed the presence of single microsatellite bands suggesting a putatively important degree of homozygosity. Molecular data were used to design a clonal seed orchard. To achieve this aim, the nine most divergent pairs of genotypes were chosen, thereby retaining 95.2% of the total number of alleles from the 140 polymorphic AFLP loci and the four microsatellite loci analyzed. Mean DI and SI for AFLP and microsatellites showed no significant differences between the original breeding population and the selected seed orchard, confirming that a seed orchard can be designed with a limited number of individuals, which allows similar accessions to be discarded and avoids inbreeding. Fil:Marcucci Poltri, S.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0829318X_v23_n9_p625_MarcucciPoltri http://hdl.handle.net/20.500.12110/paper_0829318X_v23_n9_p625_MarcucciPoltri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	AFLP Forest tree breeding Germplasm Identification matrix Microsatellite Simple sequence repeats SSR genetic variation seed Alleles Eucalyptus Forestry Genetic Markers Genotype Microsatellite Repeats Random Amplified Polymorphic DNA Technique Seeds Trees Variation (Genetics) Eucalyptus Eucalyptus dunnii
spellingShingle	AFLP Forest tree breeding Germplasm Identification matrix Microsatellite Simple sequence repeats SSR genetic variation seed Alleles Eucalyptus Forestry Genetic Markers Genotype Microsatellite Repeats Random Amplified Polymorphic DNA Technique Seeds Trees Variation (Genetics) Eucalyptus Eucalyptus dunnii Marcucci Poltri, Susana N. Hopp, Horacio Esteban Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers
topic_facet	AFLP Forest tree breeding Germplasm Identification matrix Microsatellite Simple sequence repeats SSR genetic variation seed Alleles Eucalyptus Forestry Genetic Markers Genotype Microsatellite Repeats Random Amplified Polymorphic DNA Technique Seeds Trees Variation (Genetics) Eucalyptus Eucalyptus dunnii
description	A Eucalyptus dunnii Maiden breeding population of 46 accessions originated in Australia and selected for fitness to subtropical and cold environments was screened by Amplified Fragment Length Polymorphism (AFLP) and microsatellite markers to obtain quantitative estimates of genetic diversity. A randomly chosen group of AFLP primers generated 205 AFLP bands that were used to fingerprint the genotypes and to evaluate genetic relationships among accessions. Sixty-eight percent (140) of the bands were polymorphic markers. The mean diversity index (DI) was 0.33 and about 52% of the loci had values greater than 0.4. Cluster analysis derived from similarity indices (SI) revealed no particular grouping among accessions suggesting the absence of closely related genotypes, except for five pairs of genotypes. Bootstrap analysis results confirmed the suitability of AFLP to describe genetic relationships in this breeding population. In addition, four highly informative microsatellites were used to construct an identification matrix that discriminated nearly all of the genotypes. Mean values for the number of alleles per locus, DI and SI among accessions were 13, 0.78 and 0.19, respectively, indicating that the breeding population has high genetic diversity. However, several genotypes showed the presence of single microsatellite bands suggesting a putatively important degree of homozygosity. Molecular data were used to design a clonal seed orchard. To achieve this aim, the nine most divergent pairs of genotypes were chosen, thereby retaining 95.2% of the total number of alleles from the 140 polymorphic AFLP loci and the four microsatellite loci analyzed. Mean DI and SI for AFLP and microsatellites showed no significant differences between the original breeding population and the selected seed orchard, confirming that a seed orchard can be designed with a limited number of individuals, which allows similar accessions to be discarded and avoids inbreeding.
author	Marcucci Poltri, Susana N. Hopp, Horacio Esteban
author_facet	Marcucci Poltri, Susana N. Hopp, Horacio Esteban
author_sort	Marcucci Poltri, Susana N.
title	Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers
title_short	Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers
title_full	Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers
title_fullStr	Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers
title_full_unstemmed	Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers
title_sort	selection of a seed orchard of eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0829318X_v23_n9_p625_MarcucciPoltri http://hdl.handle.net/20.500.12110/paper_0829318X_v23_n9_p625_MarcucciPoltri
work_keys_str_mv	AT marcuccipoltrisusanan selectionofaseedorchardofeucalyptusdunniibasedongeneticdiversitycriteriacalculatedusingmolecularmarkers AT hopphoracioesteban selectionofaseedorchardofeucalyptusdunniibasedongeneticdiversitycriteriacalculatedusingmolecularmarkers
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Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers

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