Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth
Yeast cells can adapt their growth in response to the nutritional environment. Glucose is the favourite carbon source of Saccharomyces cerevisiae, which prefers a fermentative metabolism despite the presence of oxygen. When glucose is consumed, the cell switches to the aerobic metabolism of ethanol,...
Guardado en:
| Publicado: |
2017
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0749503X_v34_n12_p495_Galello http://hdl.handle.net/20.500.12110/paper_0749503X_v34_n12_p495_Galello |
| Aporte de: |
| id |
paper:paper_0749503X_v34_n12_p495_Galello |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_0749503X_v34_n12_p495_Galello2023-06-08T15:45:41Z Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth Bcy1 carbon source PKA Saccharomyces cerevisiae Tpks transcription regulation cyclic AMP dependent protein kinase glucose glycerol beta galactosidase cyclic AMP dependent protein kinase fungal RNA hexokinase HXK2 protein, S cerevisiae messenger RNA MIG1 protein, S cerevisiae protein serine threonine kinase repressor protein Saccharomyces cerevisiae protein SNF1-related protein kinases Article BCY1 gene carbon source Cat8 gene controlled study fungus growth Hxk2 gene Mig1 gene nonhuman priority journal promoter region regulatory mechanism Saccharomyces cerevisiae signal transduction Snf1 gene TPK1 gene TPK2 gene TPK3 gene transcription regulation upregulation chemistry chromatin immunoprecipitation down regulation enzymology fermentation genetic transcription genetics growth, development and aging metabolism phosphorylation physiology plasmid real time polymerase chain reaction Saccharomyces cerevisiae beta-Galactosidase Chromatin Immunoprecipitation Cyclic AMP-Dependent Protein Kinases Down-Regulation Fermentation Glucose Glycerol Hexokinase Phosphorylation Plasmids Promoter Regions, Genetic Protein-Serine-Threonine Kinases Real-Time Polymerase Chain Reaction Repressor Proteins RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Transcription, Genetic Up-Regulation Yeast cells can adapt their growth in response to the nutritional environment. Glucose is the favourite carbon source of Saccharomyces cerevisiae, which prefers a fermentative metabolism despite the presence of oxygen. When glucose is consumed, the cell switches to the aerobic metabolism of ethanol, during the so-called diauxic shift. The difference between fermentative and aerobic growth is in part mediated by a regulatory mechanism called glucose repression. During glucose derepression a profound gene transcriptional reprogramming occurs and genes involved in the utilization of alternative carbon sources are expressed. Protein kinase A (PKA) controls different physiological responses following the increment of cAMP as a consequence of a particular stimulus. cAMP–PKA is one of the major pathways involved in the transduction of glucose signalling. In this work the regulation of the promoters of the PKA subunits during respiratory and fermentative metabolism are studied. It is demonstrated that all these promoters are upregulated in the presence of glycerol as carbon source through the Snf1/Cat8 pathway. However, in the presence of glucose as carbon source, the regulation of each PKA promoter subunits is different and only TPK1 is repressed by the complex Hxk2/Mig1 in the presence of active Snf1. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0749503X_v34_n12_p495_Galello http://hdl.handle.net/20.500.12110/paper_0749503X_v34_n12_p495_Galello |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bcy1 carbon source PKA Saccharomyces cerevisiae Tpks transcription regulation cyclic AMP dependent protein kinase glucose glycerol beta galactosidase cyclic AMP dependent protein kinase fungal RNA hexokinase HXK2 protein, S cerevisiae messenger RNA MIG1 protein, S cerevisiae protein serine threonine kinase repressor protein Saccharomyces cerevisiae protein SNF1-related protein kinases Article BCY1 gene carbon source Cat8 gene controlled study fungus growth Hxk2 gene Mig1 gene nonhuman priority journal promoter region regulatory mechanism Saccharomyces cerevisiae signal transduction Snf1 gene TPK1 gene TPK2 gene TPK3 gene transcription regulation upregulation chemistry chromatin immunoprecipitation down regulation enzymology fermentation genetic transcription genetics growth, development and aging metabolism phosphorylation physiology plasmid real time polymerase chain reaction Saccharomyces cerevisiae beta-Galactosidase Chromatin Immunoprecipitation Cyclic AMP-Dependent Protein Kinases Down-Regulation Fermentation Glucose Glycerol Hexokinase Phosphorylation Plasmids Promoter Regions, Genetic Protein-Serine-Threonine Kinases Real-Time Polymerase Chain Reaction Repressor Proteins RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Transcription, Genetic Up-Regulation |
| spellingShingle |
Bcy1 carbon source PKA Saccharomyces cerevisiae Tpks transcription regulation cyclic AMP dependent protein kinase glucose glycerol beta galactosidase cyclic AMP dependent protein kinase fungal RNA hexokinase HXK2 protein, S cerevisiae messenger RNA MIG1 protein, S cerevisiae protein serine threonine kinase repressor protein Saccharomyces cerevisiae protein SNF1-related protein kinases Article BCY1 gene carbon source Cat8 gene controlled study fungus growth Hxk2 gene Mig1 gene nonhuman priority journal promoter region regulatory mechanism Saccharomyces cerevisiae signal transduction Snf1 gene TPK1 gene TPK2 gene TPK3 gene transcription regulation upregulation chemistry chromatin immunoprecipitation down regulation enzymology fermentation genetic transcription genetics growth, development and aging metabolism phosphorylation physiology plasmid real time polymerase chain reaction Saccharomyces cerevisiae beta-Galactosidase Chromatin Immunoprecipitation Cyclic AMP-Dependent Protein Kinases Down-Regulation Fermentation Glucose Glycerol Hexokinase Phosphorylation Plasmids Promoter Regions, Genetic Protein-Serine-Threonine Kinases Real-Time Polymerase Chain Reaction Repressor Proteins RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Transcription, Genetic Up-Regulation Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth |
| topic_facet |
Bcy1 carbon source PKA Saccharomyces cerevisiae Tpks transcription regulation cyclic AMP dependent protein kinase glucose glycerol beta galactosidase cyclic AMP dependent protein kinase fungal RNA hexokinase HXK2 protein, S cerevisiae messenger RNA MIG1 protein, S cerevisiae protein serine threonine kinase repressor protein Saccharomyces cerevisiae protein SNF1-related protein kinases Article BCY1 gene carbon source Cat8 gene controlled study fungus growth Hxk2 gene Mig1 gene nonhuman priority journal promoter region regulatory mechanism Saccharomyces cerevisiae signal transduction Snf1 gene TPK1 gene TPK2 gene TPK3 gene transcription regulation upregulation chemistry chromatin immunoprecipitation down regulation enzymology fermentation genetic transcription genetics growth, development and aging metabolism phosphorylation physiology plasmid real time polymerase chain reaction Saccharomyces cerevisiae beta-Galactosidase Chromatin Immunoprecipitation Cyclic AMP-Dependent Protein Kinases Down-Regulation Fermentation Glucose Glycerol Hexokinase Phosphorylation Plasmids Promoter Regions, Genetic Protein-Serine-Threonine Kinases Real-Time Polymerase Chain Reaction Repressor Proteins RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Transcription, Genetic Up-Regulation |
| description |
Yeast cells can adapt their growth in response to the nutritional environment. Glucose is the favourite carbon source of Saccharomyces cerevisiae, which prefers a fermentative metabolism despite the presence of oxygen. When glucose is consumed, the cell switches to the aerobic metabolism of ethanol, during the so-called diauxic shift. The difference between fermentative and aerobic growth is in part mediated by a regulatory mechanism called glucose repression. During glucose derepression a profound gene transcriptional reprogramming occurs and genes involved in the utilization of alternative carbon sources are expressed. Protein kinase A (PKA) controls different physiological responses following the increment of cAMP as a consequence of a particular stimulus. cAMP–PKA is one of the major pathways involved in the transduction of glucose signalling. In this work the regulation of the promoters of the PKA subunits during respiratory and fermentative metabolism are studied. It is demonstrated that all these promoters are upregulated in the presence of glycerol as carbon source through the Snf1/Cat8 pathway. However, in the presence of glucose as carbon source, the regulation of each PKA promoter subunits is different and only TPK1 is repressed by the complex Hxk2/Mig1 in the presence of active Snf1. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd. |
| title |
Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth |
| title_short |
Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth |
| title_full |
Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth |
| title_fullStr |
Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth |
| title_full_unstemmed |
Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth |
| title_sort |
transcriptional regulation of the protein kinase a subunits in saccharomyces cerevisiae during fermentative growth |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0749503X_v34_n12_p495_Galello http://hdl.handle.net/20.500.12110/paper_0749503X_v34_n12_p495_Galello |
| _version_ |
1768542649182060544 |