Biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au
A new biomimetic nanostructured electrocatalyst comprised of a self-assembled monolayer (SAM) of flavin covalently attached to Au by reaction of methylformylisoalloxazine with chemisorbed cysteamine is introduced. Examinations by Fourier transform infrared spectroscopy and scanning tunneling microsc...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v21_n17_p7907_Calvo http://hdl.handle.net/20.500.12110/paper_07437463_v21_n17_p7907_Calvo |
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paper:paper_07437463_v21_n17_p7907_Calvo2023-06-08T15:44:53Z Biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au Calvo, Ernesto Julio Bonazzola, Cecilia Collisional reaction Cysteamine moieties Flavin electrocatalysts Flavin molecules Catalysis Enzymes Fourier transform infrared spectroscopy Gold Monolayers pH effects Reaction kinetics Scanning tunneling microscopy Self assembly Separation Biomimetic materials gold mercaptamine methylformylisoalloxazine nanomaterial nicotinamide adenine dinucleotide riboflavin derivative article artificial membrane biomimetics catalysis chemical structure chemistry electrochemistry infrared spectroscopy oxidation reduction reaction scanning tunneling microscopy sensitivity and specificity surface property Biomimetics Catalysis Cysteamine Electrochemistry Flavins Gold Membranes, Artificial Microscopy, Scanning Tunneling Molecular Structure NAD Nanostructures Oxidation-Reduction Sensitivity and Specificity Spectroscopy, Fourier Transform Infrared Surface Properties A new biomimetic nanostructured electrocatalyst comprised of a self-assembled monolayer (SAM) of flavin covalently attached to Au by reaction of methylformylisoalloxazine with chemisorbed cysteamine is introduced. Examinations by Fourier transform infrared spectroscopy and scanning tunneling microscopy (STM) show that the flavin molecules are oriented perpendicular to the surface with a 2 nm separation between flavin molecules. As a result of the contrast observed in the STM profiles between areas only covered by unreacted cysteamine and those covered by flavin-cysteamine moieties, it can be seen that the flavin molecules rise 0.7 nm above the chemisorbed cysteamines. The SAM flavin electrocatalyst undergoes fast electron transfer with the underlying Au and shows activity toward the oxidation of enzymatically active β-NADH at pH 7 and very low potential (-0.2 V vs Ag/AgCl), a requirement for use in an enzymatic biofuel cell, and a 100-fold increase in activity with respect to the collisional reaction in solution. © 2005 American Chemical Society. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bonazzola, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v21_n17_p7907_Calvo http://hdl.handle.net/20.500.12110/paper_07437463_v21_n17_p7907_Calvo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Collisional reaction Cysteamine moieties Flavin electrocatalysts Flavin molecules Catalysis Enzymes Fourier transform infrared spectroscopy Gold Monolayers pH effects Reaction kinetics Scanning tunneling microscopy Self assembly Separation Biomimetic materials gold mercaptamine methylformylisoalloxazine nanomaterial nicotinamide adenine dinucleotide riboflavin derivative article artificial membrane biomimetics catalysis chemical structure chemistry electrochemistry infrared spectroscopy oxidation reduction reaction scanning tunneling microscopy sensitivity and specificity surface property Biomimetics Catalysis Cysteamine Electrochemistry Flavins Gold Membranes, Artificial Microscopy, Scanning Tunneling Molecular Structure NAD Nanostructures Oxidation-Reduction Sensitivity and Specificity Spectroscopy, Fourier Transform Infrared Surface Properties |
| spellingShingle |
Collisional reaction Cysteamine moieties Flavin electrocatalysts Flavin molecules Catalysis Enzymes Fourier transform infrared spectroscopy Gold Monolayers pH effects Reaction kinetics Scanning tunneling microscopy Self assembly Separation Biomimetic materials gold mercaptamine methylformylisoalloxazine nanomaterial nicotinamide adenine dinucleotide riboflavin derivative article artificial membrane biomimetics catalysis chemical structure chemistry electrochemistry infrared spectroscopy oxidation reduction reaction scanning tunneling microscopy sensitivity and specificity surface property Biomimetics Catalysis Cysteamine Electrochemistry Flavins Gold Membranes, Artificial Microscopy, Scanning Tunneling Molecular Structure NAD Nanostructures Oxidation-Reduction Sensitivity and Specificity Spectroscopy, Fourier Transform Infrared Surface Properties Calvo, Ernesto Julio Bonazzola, Cecilia Biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au |
| topic_facet |
Collisional reaction Cysteamine moieties Flavin electrocatalysts Flavin molecules Catalysis Enzymes Fourier transform infrared spectroscopy Gold Monolayers pH effects Reaction kinetics Scanning tunneling microscopy Self assembly Separation Biomimetic materials gold mercaptamine methylformylisoalloxazine nanomaterial nicotinamide adenine dinucleotide riboflavin derivative article artificial membrane biomimetics catalysis chemical structure chemistry electrochemistry infrared spectroscopy oxidation reduction reaction scanning tunneling microscopy sensitivity and specificity surface property Biomimetics Catalysis Cysteamine Electrochemistry Flavins Gold Membranes, Artificial Microscopy, Scanning Tunneling Molecular Structure NAD Nanostructures Oxidation-Reduction Sensitivity and Specificity Spectroscopy, Fourier Transform Infrared Surface Properties |
| description |
A new biomimetic nanostructured electrocatalyst comprised of a self-assembled monolayer (SAM) of flavin covalently attached to Au by reaction of methylformylisoalloxazine with chemisorbed cysteamine is introduced. Examinations by Fourier transform infrared spectroscopy and scanning tunneling microscopy (STM) show that the flavin molecules are oriented perpendicular to the surface with a 2 nm separation between flavin molecules. As a result of the contrast observed in the STM profiles between areas only covered by unreacted cysteamine and those covered by flavin-cysteamine moieties, it can be seen that the flavin molecules rise 0.7 nm above the chemisorbed cysteamines. The SAM flavin electrocatalyst undergoes fast electron transfer with the underlying Au and shows activity toward the oxidation of enzymatically active β-NADH at pH 7 and very low potential (-0.2 V vs Ag/AgCl), a requirement for use in an enzymatic biofuel cell, and a 100-fold increase in activity with respect to the collisional reaction in solution. © 2005 American Chemical Society. |
| author |
Calvo, Ernesto Julio Bonazzola, Cecilia |
| author_facet |
Calvo, Ernesto Julio Bonazzola, Cecilia |
| author_sort |
Calvo, Ernesto Julio |
| title |
Biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au |
| title_short |
Biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au |
| title_full |
Biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au |
| title_fullStr |
Biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au |
| title_full_unstemmed |
Biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au |
| title_sort |
biomimetics with a self-assembled monolayer of catalytically active tethered isoalloxazine on au |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v21_n17_p7907_Calvo http://hdl.handle.net/20.500.12110/paper_07437463_v21_n17_p7907_Calvo |
| work_keys_str_mv |
AT calvoernestojulio biomimeticswithaselfassembledmonolayerofcatalyticallyactivetetheredisoalloxazineonau AT bonazzolacecilia biomimeticswithaselfassembledmonolayerofcatalyticallyactivetetheredisoalloxazineonau |
| _version_ |
1768546165462138880 |