The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate
The involvement of phospholipids in the activity of 5αreductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5αreductase in both subcellular fractions; this effect was not attributable to the hydrolysis produc...
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1977
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v4_n2_p85_Charreau http://hdl.handle.net/20.500.12110/paper_07435800_v4_n2_p85_Charreau |
| Aporte de: |
| Sumario: | The involvement of phospholipids in the activity of 5αreductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5αreductase in both subcellular fractions; this effect was not attributable to the hydrolysis products. Phospholipase C stopped only the activity of the nuclear fraction. There was no quantitative correlation between hydrolysis of phospholipids and loss of enzyme activity. The inactivation observed seems to result from phospholipase-induced instability of the enzyme. The results suggest that the altered structure of phospholipids and/or the membranes where 5αreductase is located is responsible for the decrease in activity. © 1977 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
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