The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate
The involvement of phospholipids in the activity of 5αreductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5αreductase in both subcellular fractions; this effect was not attributable to the hydrolysis produc...
Guardado en:
| Publicado: |
1977
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v4_n2_p85_Charreau http://hdl.handle.net/20.500.12110/paper_07435800_v4_n2_p85_Charreau |
| Aporte de: |
| id |
paper:paper_07435800_v4_n2_p85_Charreau |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_07435800_v4_n2_p85_Charreau2023-06-08T15:44:46Z The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate phospholipase phospholipase a phospholipase c phospholipid radioisotope steroid reductase 5alpha androstane 3alpha,17beta diol c 14 androstanolone c 14 membrane structure microsome prostate rat testosterone h 3 theoretical study Animal Cell Nucleus Male Microsomes Oxidoreductases Phospholipases Phospholipids Prostate Rats Serum Albumin, Bovine Testosterone 5-alpha-Reductase The involvement of phospholipids in the activity of 5αreductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5αreductase in both subcellular fractions; this effect was not attributable to the hydrolysis products. Phospholipase C stopped only the activity of the nuclear fraction. There was no quantitative correlation between hydrolysis of phospholipids and loss of enzyme activity. The inactivation observed seems to result from phospholipase-induced instability of the enzyme. The results suggest that the altered structure of phospholipids and/or the membranes where 5αreductase is located is responsible for the decrease in activity. © 1977 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v4_n2_p85_Charreau http://hdl.handle.net/20.500.12110/paper_07435800_v4_n2_p85_Charreau |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
phospholipase phospholipase a phospholipase c phospholipid radioisotope steroid reductase 5alpha androstane 3alpha,17beta diol c 14 androstanolone c 14 membrane structure microsome prostate rat testosterone h 3 theoretical study Animal Cell Nucleus Male Microsomes Oxidoreductases Phospholipases Phospholipids Prostate Rats Serum Albumin, Bovine Testosterone 5-alpha-Reductase |
| spellingShingle |
phospholipase phospholipase a phospholipase c phospholipid radioisotope steroid reductase 5alpha androstane 3alpha,17beta diol c 14 androstanolone c 14 membrane structure microsome prostate rat testosterone h 3 theoretical study Animal Cell Nucleus Male Microsomes Oxidoreductases Phospholipases Phospholipids Prostate Rats Serum Albumin, Bovine Testosterone 5-alpha-Reductase The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate |
| topic_facet |
phospholipase phospholipase a phospholipase c phospholipid radioisotope steroid reductase 5alpha androstane 3alpha,17beta diol c 14 androstanolone c 14 membrane structure microsome prostate rat testosterone h 3 theoretical study Animal Cell Nucleus Male Microsomes Oxidoreductases Phospholipases Phospholipids Prostate Rats Serum Albumin, Bovine Testosterone 5-alpha-Reductase |
| description |
The involvement of phospholipids in the activity of 5αreductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5αreductase in both subcellular fractions; this effect was not attributable to the hydrolysis products. Phospholipase C stopped only the activity of the nuclear fraction. There was no quantitative correlation between hydrolysis of phospholipids and loss of enzyme activity. The inactivation observed seems to result from phospholipase-induced instability of the enzyme. The results suggest that the altered structure of phospholipids and/or the membranes where 5αreductase is located is responsible for the decrease in activity. © 1977 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
| title |
The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate |
| title_short |
The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate |
| title_full |
The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate |
| title_fullStr |
The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate |
| title_full_unstemmed |
The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate |
| title_sort |
role of phospholipids in the activity of a4-3-ket0steroid 5αoxidoreductase of the rat ventral prostate |
| publishDate |
1977 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v4_n2_p85_Charreau http://hdl.handle.net/20.500.12110/paper_07435800_v4_n2_p85_Charreau |
| _version_ |
1768543951279620096 |