Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster
Alcohol, a drug widely abused, impacts the central nervous system functioning of diverse organisms. The behavioral responses to acute alcohol exposure are remarkably similar among humans and fruit flies. In its natural environment, rich in fermentation products, the fruit fly Drosophila melanogaster...
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paper:paper_07418329_v46_n8_p737_Defays2023-06-08T15:44:40Z Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster Defays, Raquel Bertoli, Carlos Ignacio Alcohol Chemical stress Ethanol resistance Ethanol-coma recovery QTL RIL alcohol adaptive behavior animal experiment article chromosome analysis controlled study Drosophila melanogaster female gene interaction gene locus genetic identification genetic trait genetic variability insect genome male nonhuman phenotype quantitative trait locus mapping recombinant inbred strain survival rate Animals Animals, Inbred Strains Drosophila melanogaster Drosophila Proteins Ethanol Female Gene Knockdown Techniques Male Quantitative Trait Loci Random Allocation Recombinant Proteins Drosophila melanogaster Alcohol, a drug widely abused, impacts the central nervous system functioning of diverse organisms. The behavioral responses to acute alcohol exposure are remarkably similar among humans and fruit flies. In its natural environment, rich in fermentation products, the fruit fly Drosophila melanogaster encounters relatively high levels of ethanol. The effects of ethanol and its metabolites on Drosophila have been studied for decades, as a model for adaptive evolution. Although extensive work has been done for elucidating patterns of genetic variation, substantially less is known about the genomic regions or genes that underlie the genetic variation of this important trait. To identify regions containing genes involved in the responses to ethanol, we used a mapping population of recombinant inbred (RIL) lines to map quantitative trait loci (QTL) that affect variation in resistance and recovery from ethanol sedation in adults and ethanol resistance in larvae. We mapped fourteen QTL affecting the response to ethanol on the three chromosomes. Seven of the QTL influence the resistance to ethanol in adults, two QTL are related to ethanol-coma recovery in adults and five affect the survival to ethanol in larvae. Most of the QTL were trait specific, suggesting that overlapping but generally unique genetic architectures underlie each trait. Each QTL explained up to 16.8% of the genetic variance among lines. Potential candidate loci contained within our QTL regions were identified and analyzed. © 2012 Elsevier Inc. Fil:Defays, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bertoli, C.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07418329_v46_n8_p737_Defays http://hdl.handle.net/20.500.12110/paper_07418329_v46_n8_p737_Defays |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alcohol Chemical stress Ethanol resistance Ethanol-coma recovery QTL RIL alcohol adaptive behavior animal experiment article chromosome analysis controlled study Drosophila melanogaster female gene interaction gene locus genetic identification genetic trait genetic variability insect genome male nonhuman phenotype quantitative trait locus mapping recombinant inbred strain survival rate Animals Animals, Inbred Strains Drosophila melanogaster Drosophila Proteins Ethanol Female Gene Knockdown Techniques Male Quantitative Trait Loci Random Allocation Recombinant Proteins Drosophila melanogaster |
| spellingShingle |
Alcohol Chemical stress Ethanol resistance Ethanol-coma recovery QTL RIL alcohol adaptive behavior animal experiment article chromosome analysis controlled study Drosophila melanogaster female gene interaction gene locus genetic identification genetic trait genetic variability insect genome male nonhuman phenotype quantitative trait locus mapping recombinant inbred strain survival rate Animals Animals, Inbred Strains Drosophila melanogaster Drosophila Proteins Ethanol Female Gene Knockdown Techniques Male Quantitative Trait Loci Random Allocation Recombinant Proteins Drosophila melanogaster Defays, Raquel Bertoli, Carlos Ignacio Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| topic_facet |
Alcohol Chemical stress Ethanol resistance Ethanol-coma recovery QTL RIL alcohol adaptive behavior animal experiment article chromosome analysis controlled study Drosophila melanogaster female gene interaction gene locus genetic identification genetic trait genetic variability insect genome male nonhuman phenotype quantitative trait locus mapping recombinant inbred strain survival rate Animals Animals, Inbred Strains Drosophila melanogaster Drosophila Proteins Ethanol Female Gene Knockdown Techniques Male Quantitative Trait Loci Random Allocation Recombinant Proteins Drosophila melanogaster |
| description |
Alcohol, a drug widely abused, impacts the central nervous system functioning of diverse organisms. The behavioral responses to acute alcohol exposure are remarkably similar among humans and fruit flies. In its natural environment, rich in fermentation products, the fruit fly Drosophila melanogaster encounters relatively high levels of ethanol. The effects of ethanol and its metabolites on Drosophila have been studied for decades, as a model for adaptive evolution. Although extensive work has been done for elucidating patterns of genetic variation, substantially less is known about the genomic regions or genes that underlie the genetic variation of this important trait. To identify regions containing genes involved in the responses to ethanol, we used a mapping population of recombinant inbred (RIL) lines to map quantitative trait loci (QTL) that affect variation in resistance and recovery from ethanol sedation in adults and ethanol resistance in larvae. We mapped fourteen QTL affecting the response to ethanol on the three chromosomes. Seven of the QTL influence the resistance to ethanol in adults, two QTL are related to ethanol-coma recovery in adults and five affect the survival to ethanol in larvae. Most of the QTL were trait specific, suggesting that overlapping but generally unique genetic architectures underlie each trait. Each QTL explained up to 16.8% of the genetic variance among lines. Potential candidate loci contained within our QTL regions were identified and analyzed. © 2012 Elsevier Inc. |
| author |
Defays, Raquel Bertoli, Carlos Ignacio |
| author_facet |
Defays, Raquel Bertoli, Carlos Ignacio |
| author_sort |
Defays, Raquel |
| title |
Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_short |
Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_full |
Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_fullStr |
Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_full_unstemmed |
Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_sort |
quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of drosophila melanogaster |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07418329_v46_n8_p737_Defays http://hdl.handle.net/20.500.12110/paper_07418329_v46_n8_p737_Defays |
| work_keys_str_mv |
AT defaysraquel quantitativetraitlociforresponsetoethanolinanintercontinentalsetofrecombinantinbredlinesofdrosophilamelanogaster AT bertolicarlosignacio quantitativetraitlociforresponsetoethanolinanintercontinentalsetofrecombinantinbredlinesofdrosophilamelanogaster |
| _version_ |
1768546305848639488 |